[en] Our technological goal was to test the new technology of DNA microarrays in the field of biodiversity assessments. The efficiency of DNA microarrays; was compared against the "classical" methods for measuring and monitoring the biodiversity. We use cyanobacteria from freshwater lakes as model organisms belonging to a well defined phylum of much ecological relevance. The combination of the information about cyanobacterial biodiversity together with the data describing the water quality of the lakes may reveal useful information to design management actions to control the unwanted mass developments of cyanobacteria. Such blooms prevent the use of water for drinking and for recreation and can be toxic to animals and humans. The possibility to automate the microarray technology in water quality monitoring will be an interesting alternative to time-consuming microscopic enumerations.
Castenholz RW, Waterbury JB. Group I. Cyanobacteria. In: Boone DR, Castenholz RW, editors. Bergey's manual of systematic bacteriology. Vol. 3. New York: Springer-Verlag; 2001.
Rippka R. Isolation and purification of cyanobacteria. Methods Enzymol 1988;167:3-27.
Turner S. Molecular systematics of oxygenic photosynthetic bacteria. In: Bhattacharya D, editor. Origins of algae and their plastids. New York: Springer-Verlag; 1998.
Ferris MJ, Muyzer G, Ward DM. Denaturing gradient gel electrophoresis profiles of 16S rRNA-defined populations inhabiting a hot spring microbial mat community. Appl Environ Microbiol 1996;66:499-508.
Muyzer G. DGGE/TGGE a method for identifying genes from natural ecosystems. Curr Opin Microbiol 1999;2:317-22.
Nubel U, Garcia-Pichel F, Kuhl M, Muyzer G. Quantifying microbial diversities: morphotypes, 16S rRNA genes and carotenoids of oxygenic phototrophs in microbial mats. Appl Environ Microbiol 1999;65:422-30.
Gerry NP, Witowski NE, Day J, Hammer RP, Barany G, Barany F. Universal DNA microarray method for multiplex detection of low abundance point mutations. J Mol Biol 1999;292:251-62.
