evernic acid; ITS barcodes; lichen; Ramalina carminae; taxonomy
Abstract :
[en] Ramalina arsenii sp. nov. belongs to the R. pollinaria group and is easily recognized by its ITS barcode and several micro-morphological
characters that are diagnostic in a European context: small size, less than 3 cm long; soralia developing on the underside of lobe apices;
absence of excavate depressions on the lower side. Its ecological niche (i.e. rock outcrops and especially underhangs of slightly calcareous
rocks, at low and mid altitudes) is also unique. Ramalina arsenii is frequent and locally abundant in France (Alps, Cantal) and Switzerland
(western Alps), and is also known from Germany and the Spanish side of the Pyrenees.
Sérusiaux, Emmanuël ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Océanographie biologique
Van den Boom, Pieter
Magain, Nicolas ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Biologie de l'évolution et gestion de la biodiversité
Language :
English
Title :
Ramalina arsenii, an additional new species in the R. pollinaria group in Western Europe
Arroyo R, Seriñá E and Araujo E (2011) Ramalina carminae (Ascomycota: Ramalinaceae), a new species from Europe. Botanica Complutensis 35, 5-14. 10.5209/rev_BOCM.2011.v35.1
Boluda CG, Rico VJ, Divakar PK, Nadyeina O, Myllys L, McMullin RT, Zamora JC, Scheidegger C and Hawksworth DL (2019) Evaluating methodologies for species delimitation: the mismatch between phenotypes and genotypes in lichenized fungi (Bryoria sect. Implexae, Parmeliaceae). Persoonia 42, 75-100. 10.3767/persoonia.2019.42.04
Cannon P, Ekman S, Kistenich S, LaGreca S, Printzen C, Timdal E, Aptroot A, Coppins B, Fletcher A, Sanderson N, et al., (2021) Lecanorales: Ramalinaceae, including the genera Bacidia, Bacidina, Bellicidia, Biatora, Bibbya, Bilimbia, Cliostomum, Kiliasia, Lecania, Megalaria, Mycobilimbia, Phyllopsora, Ramalina, Scutula, Thalloidima, Toninia, Toniniopsis and Tylothallia. Revisions of British and Irish Lichens 11, 1-82.
Clement M, Posada D and Crandall KA (2000) TCS: a computer program to estimate gene genealogies. Molecular Ecology 9, 1657-1659. 10.1046/j.1365-294x.2000.01020.x
Cubero OF, Crespo A, Fatehi J and Bridge PD (1999) DNA extraction and PCR amplification method suitable for fresh, herbarium-stored, lichenized, and other fungi. Plant Systematics and Evolution 216, 243-249. 10.1007/BF01084401
Gardes M and Bruns TD (1993) ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts. Molecular Ecology 2, 113-118.
Gasparyan A and Sipman HJ (2016) The epiphytic lichenized fungi in Armenia: diversity and conservation. Phytotaxa 281, 1-68. 10.11646/phytotaxa.281.1.1
Gasparyan A, Sipman HJ and Lücking R (2017) Ramalina europaea and R. labiosorediata, two new species of the R. pollinaria group (Ascomycota: Ramalinaceae), and new typifications for Lichen pollinarius and L. squarrosus. Lichenologist 49, 301-319.
Katoh K, Asimenos G and Toh H (2009) Multiple alignment of DNA sequences with MAFFT. In Posada D (ed.), Bioinformatics for DNA Sequence Analysis. New York: Humana Press, pp. 39-64.
Krog H and Osthagen H (1980) The genus Ramalina in the Canary Islands. Norwegian Journal of Botany 27, 255-296.
LaGreca S, Lumbsch HT, Kukwa M, Wei X, Han JE, Moon KH, Kashiwadani H, Aptroot A and Leavitt SD (2020) A molecular phylogenetic evaluation of the Ramalina siliquosa complex, with notes on species circumscription and relationships within Ramalina. Lichenologist 52, 197-211.
Lücking R, Dal-Forno M, Sikaroodi M, Gillevet PM, Bungartz F, Moncada B, Yánez-Ayabaca A, Chaves JL, Coca LF and Lawrey JD (2014) A single macrolichen constitutes hundreds of unrecognized species. Proceedings of the National Academy of Sciences of the United States of America 111, 11091-11096.
Miadlikowska J, Magain N, Pardo de la Hoz C, Niu D, Goward T, Sérusiaux E and Lutzoni F (2018) Species in section Peltidea (aphthosa group) of the genus Peltigera remain cryptic after molecular phylogenetic revision. Plant and Fungal Systematics 63, 45-64.
Nimis PL (2016) The Lichens of Italy. A Second Annotated Catalogue. Trieste: EUT (Edizioni Università di Trieste).
Orange A, James PW and White FJ (2010) Microchemical Methods for the Identification of Lichens. London: British Lichen Society.
Peay KG (2016) The mutualistic niche: mycorrhizal symbiosis and community dynamics. Annual Review of Ecology, Evolution, and Systematics 47, 143-164.
Pérez-Ortega S, Pérez-Vargas I, Blázquez M, Garrido-Benavent I, Aptroot A, Bungartz F, Blanchon D, Cáceres MES, Divakar PK, Ertz D, et al., (2019) First genus-wide phylogeny of the genus Ramalina (lichenized Ascomycota) sheds light on the endemic diversity in Macaronesia, Abstracts of the Island Biology III International Conference, 8-13 July 2019, Saint Denis, La Réunion, France, pp. 314-315.
Saag L, Mark K, Saag A and Randlane T (2014) Species delimitation in the lichenized fungal genus Vulpicida (Parmeliaceae, Ascomycota) using gene concatenation and coalescent-based species tree approaches. American Journal of Botany 101, 2169-2182.
Sérusiaux E, van den Boom P and Ertz D (2010) A two-gene phylogeny shows the lichen genus Niebla (Lecanorales) is endemic to the New World and does not occur in Macaronesia nor in the Mediterranean basin. Fungal Biology 114, 528-537. 10.1016/j.funbio.2010.04.002
Sheard JW (1978) The taxonomy of the Ramalina siliquosa species aggregate (lichenized Ascomycetes). Canadian Journal of Botany 56, 916-938.
Simon A, Goffinet B, Wang LS, Spribille T, Goward T, Pystina T, Semenova N, Stepanov V, Moncada B, Lücking R, et al., (2021) Shedding light on false moon lichens: a global phylogeny and taxonomic reassessment of the genus Dendricosticta (Ascomycota: Peltigerales). Taxon [in press].
Spjut R, Simon A, Guissard M, Magain N and Sérusiaux E (2020) The fruticose genera in the Ramalinaceae (Ascomycota, Lecanoromycetes): their diversity and evolutionary history. MycoKeys 73, 1-68.
Stamatakis A (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22, 2688-2690.
Stenroos S, Velmala S, Pykälä J and Ahti T (2016) Lichens of Finland. Helsinki: Finnish Museum of Natural History.
Taylor DL, Hollingsworth TN, McFarland JW, Lennon NJ, Nusbaum C and Ruess RW (2014) A first comprehensive census of fungi in soil reveals both hyperdiversity and fine-scale niche partitioning. Ecological Monographs 84, 3-20. 10.1890/12-1693.1
Templeton A, Crandall K and Sing C (1992) A cladistic analysis of phenotypic associations with haplotypes inferred from restriction endonuclease mapping and DNA sequence data. III. Cladogram estimation. Genetics 132, 619-633. 10.1093/genetics/132.2.619
Truong C and Clerc P (2016) New species and new records in the genus Usnea (Parmeliaceae, lichenized Ascomycota) from tropical South America. Lichenologist 48, 71-93. 10.1017/S0024282915000419
Truong C, Mujic AB, Healy R, Kuhar F, Furci G, Torres D, Niskanen T, Sandoval-Leiva PA, Fernández N, Escobar JM, et al., (2017) How to know the fungi: combining field inventories and DNA-barcoding to document fungal diversity. New Phytologist 214, 913-919. 10.1111/nph.14509
Wheeler DL, Barrett T, Benson DA, Bryant SH, Canese K, Chetvernin V, Church DM, DiCuccio M, Edgar R, Federhen S, et al., (2007) Database resources of the National Center for Biotechnology Information. Nucleic Acids Research 35, D5-D12. 10.1093/nar/gkl1031
White TJ, Bruns S, Lee S and Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In Innis MA, Gelfand DH, Sninsky JJ and White TJ (eds), PCR Protocols: a Guide to Methods and Applications. New York: Academic Press, pp. 315-322.