[en] Bovine viral diarrhea virus (BVDV) genomic RNA was identified as a 12.5-kb single-stranded RNA molecule in both infected bovine embryonic kidney cells (BEK-1) and partially purified virions. BVD virion RNA was partially purified and used as a template for cDNA synthesis. BVDV-specific cDNA sequences were molecularly cloned and shown to hybridize to infected cell RNA but not to uninfected cell RNA or DNA. A single RNA species of 12.5 kb, representing the viral RNA genome, was detected in infected cells. A preliminary map of the BVDV specific cDNA clones was constructed and five major, nonoverlapping families were observed, accounting for approximately one-half of the viral genome.
Disciplines :
Biochemistry, biophysics & molecular biology Veterinary medicine & animal health
Author, co-author :
Renard, A.; Université de Liège - ULiège > Laboratoire central de Génie Génétique
Guiot, C.; Université de Liège - ULiège > Laboratoire central de Génie Génétique
Schmetz, D.; Université de Liège - ULiège > Laboratoire central de Génie Génétique
Dagenais, L.; Université de Liège - ULiège > Laboratoire central de Génie Génétique
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
Bibliography
CHIRGWIN, J.M., PZYBYLA, A.E., MacDONALD, RJ., and RUTTER, WJ. (1979). Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18, 5294–5299.
ENGLAND, T.E., BRUCE, A.G., and ULHENBECK, O.C. (1980). Specific labeling of 3′ termini of RNA with T4 RNA ligase. Methods Enzymol. 65, 65–74.
KESHET, E., ROSNER, A., BERNSTEIN, Y., GORECKI, M., and AVIV, H. (1981). Cloning of bovine growth hormone gene and its expression in bacteria. Nucleic Acids Res. 9, 19–30.
KRUG M., and UHLENBECK, O.C. (1982). Reversal of T4 RNA ligase. Biochemistry 21, 1858–1864.
LEHRACH, H., DIAMOND D., WOZNEY, J.M., and BOEDTKER, G. (1977). RNA molecular weight determinations by gel electrophoresis under denaturing conditions; acritical re-examination. Biochemistry 16, 4743–4751.
MANIATIS, T., FRITSCH, E.F., and SAMBROOK, J. (1982). Molecular Cloning: A laboratory manual (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York).
MILLER, W.L., MARTIAL, J.A., and BAXTER, J.D. (1980). Molecular cloning of DNA complementary to bovine growth hormone mRNA. J. Biol Chem 255, 7521–7524.
NUTTALL, P.A. (1978). “Cytopathic and non-cytopathic mucosal disease virus.” Ph.D. Thesis, University of Reading, England.
PURCHIO, A.F., LARSON, R., and COLLETT, M.S. (1983). Characterization of virus-specific RNA synthesized in bovine cells infected with bovine viral diarrhea virus. J. Virol 48, 320–324.
SCHLESINGER, R.W., Ed. (1980). The Togaviruses. Biology, Structure, Replication (Academic Press, Inc., New York).
SIPPEL, A.E. (1973). Purification and characterization of adenosine triphosphate: Ribonucleic acid adenyltransferase from Escherichia coli. Eur. J. Biochem. 37, 31–40.
SMILEY, G.S.T., BRINK, C.F., and PEARLMAN, R.E. (1983). Hybridization of nucleic acids directly in agarose gels. Anal. Biochem. 131, 365–372.
WENGLER, G. and WENGLER, G. (1981). Terminal sequences of the genome and replicative-form RNA of the flavivirus West Nile virus: Absence of poly(A) and possible role in RNA replication. Virology 113, 544–555.
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