[en] Bovine viral diarrhea virus (BVDV) genomic RNA was identified as a 12.5-kb single-stranded RNA molecule in both infected bovine embryonic kidney cells (BEK-1) and partially purified virions. BVD virion RNA was partially purified and used as a template for cDNA synthesis. BVDV-specific cDNA sequences were molecularly cloned and shown to hybridize to infected cell RNA but not to uninfected cell RNA or DNA. A single RNA species of 12.5 kb, representing the viral RNA genome, was detected in infected cells. A preliminary map of the BVDV specific cDNA clones was constructed and five major, nonoverlapping families were observed, accounting for approximately one-half of the viral genome.
Disciplines :
Biochemistry, biophysics & molecular biology Veterinary medicine & animal health
Author, co-author :
Renard, A.; Université de Liège - ULiège > Laboratoire central de Génie Génétique
Guiot, C.; Université de Liège - ULiège > Laboratoire central de Génie Génétique
Schmetz, D.; Université de Liège - ULiège > Laboratoire central de Génie Génétique
Dagenais, L.; Université de Liège - ULiège > Laboratoire central de Génie Génétique
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