Interleukin 8 and pentaxin (C-reactive protein) as potential new biomarkers of bovine tuberculosis

Gao, X.; Guo, X.; Li, M.; Jia, H.; Lin, Weidong; Fang, L.; Jiang, Y.; Zhu, H.; Zhang, Z.; Ding, J.; Xin, T.

doi:10.1128/JCM.00274-19

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Interleukin 8 and pentaxin (C-reactive protein) as potential new biomarkers of bovine tuberculosis

Gao, X.; Guo, X.; Li, M. et al.

2019 • In Journal of Clinical Microbiology, 57 (10), p. 00274-19

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https://hdl.handle.net/2268/266446

DOI
10.1128/JCM.00274-19

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31340991

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Keywords :

Biomarker; Bovine tuberculosis; CRP; IL-8; Proteomics; C reactive protein; Article; Mycobacterium avium subsp. paratuberculosis; Mycobacterium bovis

Abstract :

[en] Bovine tuberculosis (bTB) is caused by Mycobacterium bovis. During the early stage of infection, greater than 15% of M. bovis-infected cattle shed mycobacteria through nasal secretions, which can be detected by nested PCR. To compare the differences in the protein profiles of M. bovis-infected cattle that were nested PCR positive (bTBPCR-P) and M. bovis-infected cattle that were nested PCR negative (bTBPCR-N) and to screen for biomarkers that will facilitate the early and accurate detection of bTB, we investigated the protein expression profiles of serum and bovine purified protein derivative (PPD-B)-stimulated plasma among bTBPCR-P (n = 20), bTBPCR-N (n = 20), and uninfected cattle (NC; n = 20) by iTRAQ labeling coupled with two-dimensional liquid chromatography-tandem mass spectrometry (iTRAQ-2D LC-MS/ MS). After comprehensive analysis, we selected 15 putative differentially expressed serum proteins and 15 plasma proteins for validation by parallel reaction monitoring (PRM) with the same cohort used in the iTRAQ analysis. Four serum and five PPD-B-stimulated proteins were confirmed in follow-up enzyme-linked immunosorbent assays. PPD-B-stimulated interleukin 8 (IL-8) displayed the potential to differentiate M. bovisinfected cattle from NC, with an area under the curve (AUC) value of 0.9662, while PPD-B-stimulated C-reactive protein (CRP) displayed the potential to differentiate bTBPCR-P from bTBPCR-N, with an AUC value of 1.00. Finally, double-blind testing with 244 cattle indicated that the PPD-B-stimulated IL-8 test exhibited good agreement with traditional tests (κ > 0.877) with a >90% relative sensitivity and a >98% relative specificity; the PPD-B-stimulated CRP test displayed good agreement with nested PCR (κ = 0.9117), with an observed 94% relative sensitivity and 97% relative specificity. Therefore, the PPD-B-stimulated IL-8 and CRP tests could be used to detect bTB and to differentiate bTBPCR-P from bTBPCR-N. Copyright © 2019 Gao et al.

Disciplines :

Microbiology

Author, co-author :

Gao, X.; Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China

Guo, X.; Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China

Li, M.; Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China

Jia, H.; Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China

Lin, Weidong ; Université de Liège - ULiège > Terra

Fang, L.; Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China

Jiang, Y.; Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China

Zhu, H.; Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China

Zhang, Z.; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China

Ding, J.; China Institute of Veterinary Drugs Control, Beijing, China

Xin, T.; Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China

Language :

English

Title :

Interleukin 8 and pentaxin (C-reactive protein) as potential new biomarkers of bovine tuberculosis

Publication date :

2019

Journal title :

Journal of Clinical Microbiology

ISSN :

0095-1137

eISSN :

1098-660X

Publisher :

American Society for Microbiology, United States - District of Columbia

Volume :

Issue :

Pages :

e00274-19

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 26 December 2021

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