Article (Scientific journals)
Old Enzyme, New Role: The β-Glucosidase BglC of Streptomyces scabiei Interferes with the Plant Defense Mechanism by Hydrolyzing Scopolin
Deflandre, Benoit; Rigali, Sébastien
2021In Biophysica, 2 (1), p. 1-7
Peer Reviewed verified by ORBi
 

Files


Full Text
Deflandfre et al. Biophysica 2021.pdf
Publisher postprint (1.38 MB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
common scab disease; plant defense mechanism; beta-glucosidase
Abstract :
[en] The beta-glucosidase BglC fulfills multiple functions in both primary metabolism and induction of pathogenicity of Streptomyces scabiei, the causative agent of common scab in root and tuber crops. Indeed, this enzyme hydrolyzes cellobiose and cellotriose to feed glycolysis with glucose directly and modifies the intracellular concentration of these cello-oligosaccharides, which are the virulence elicitors. The inactivation of bglC led to unexpected phenotypes such as the constitutive overproduction of thaxtomin A, the main virulence determinant of S. scabiei. In this work, we reveal a new target substrate of BglC, the phytoalexin scopolin. Removal of the glucose moiety of scopolin generates scopoletin, a potent inhibitor of thaxtomin A production. The hydrolysis of scopolin by BglC displayed substrate inhibition kinetics, which contrasts with the typical Michaelis–Menten saturation curve previously observed for the degradation of its natural substrate cellobiose. Our work, therefore, reveals that BglC targets both cello-oligosaccharide elicitors emanating from the hosts of S. scabiei, and the scopolin phytoalexin generated by the host defense mechanisms, thereby occupying a key position to fine-tune the production of the main virulence determinant thaxtomin A.
Disciplines :
Microbiology
Microbiology
Author, co-author :
Deflandre, Benoit ;  Université de Liège - ULiège > InBioS
Rigali, Sébastien  ;  Université de Liège - ULiège > Département des sciences de la vie > Centre d'ingénierie des protéines
Language :
English
Title :
Old Enzyme, New Role: The β-Glucosidase BglC of Streptomyces scabiei Interferes with the Plant Defense Mechanism by Hydrolyzing Scopolin
Publication date :
December 2021
Journal title :
Biophysica
eISSN :
2673-4125
Volume :
2
Issue :
1
Pages :
1-7
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 23 December 2021

Statistics


Number of views
75 (7 by ULiège)
Number of downloads
42 (3 by ULiège)

Scopus citations®
 
5
Scopus citations®
without self-citations
4
OpenCitations
 
3

Bibliography


Similar publications



Contact ORBi