Determination of thyreostats in urine using supported liquid extraction and mixed-mode cation-exchange solid-phase extraction: Screening and confirmatory methods

Tölgyesi, Á.; Giri, Anupam; Barta, E.; McDonald, T. J.; Sharma, V. K.

doi:10.1093/chromsci/bmy054

Article (Scientific journals)

Determination of thyreostats in urine using supported liquid extraction and mixed-mode cation-exchange solid-phase extraction: Screening and confirmatory methods

Tölgyesi, Á.; Giri, Anupam; Barta, E. et al.

2018 • In Journal of Chromatographic Science, 56 (9), p. 858-866

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/265952

DOI
10.1093/chromsci/bmy054

PubMed
29878072

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Keywords :

Animals; Antithyroid Agents; Cattle; Chromatography, High Pressure Liquid; Limit of Detection; Liquid-Liquid Extraction; Methimazole; Reproducibility of Results; Solid Phase Extraction; Swine; Tandem Mass Spectrometry; Thiouracil

Abstract :

[en] The application of thyreostats in livestock has been banned in the European Union since 1981, but these drugs are currently in the focus due to the natural occurrence of thiouracil (TU). Studies have been published on TU contamination in urine samples of animal and human origins without any drug administration of it. This paper presents new analytical methods to analyze thyreostats to support the legislation on the recommended concentration (RC) levels of these drugs. Both screening and confirmatory methods are developed for analyzing thyreostats in porcine and bovine urines using a liquid chromatography-tandem mass spectrometry technique. The new methods include a chemical derivatization with 3-iodobenzyl bromide, followed by novel purification approaches using supported liquid extraction and mixed-mode cation-exchange solid-phase extraction (SPE) for screening and confirmatory purposes, respectively. The optimized derivatization in combination with the cation-exchange SPE gives high sensitivity and reducing matrix effect of the analysis. The methods are validated in accordance with the guidelines for the validation of screening methods and European Commission Decision 2002/657/EC. The confirmatory method is used in the national monitoring plan. The detected levels of TU in urine samples are below the currently applicable RC level (10 μg L−1). © The Author(s) 2018. Published by Oxford University Press. All rights reserved.

Disciplines :

Chemistry

Author, co-author :

Tölgyesi, Á.; National Food Chain Safety Office, Food and Feed Safety Dirct., Food Toxicological National Reference Laboratory, Mester utca 81, Budapest, 1095, Hungary

Giri, Anupam ; Université de Liège - ULg

Barta, E.; Budapest University of Technology and Economics, Department of Inorganic and Analytical Chemistry, Szt. Gellért tér 4, Budapest, 1111, Hungary

McDonald, T. J.; Program for the Environment and Sustainability, Department of Environmental and Occupational Health, School of Public Health, Texas A and M University, 1266 TAMU, 212 Adriance Lab Road, College Station, TX 77843, United States

Sharma, V. K.; Program for the Environment and Sustainability, Department of Environmental and Occupational Health, School of Public Health, Texas A and M University, 1266 TAMU, 212 Adriance Lab Road, College Station, TX 77843, United States

Language :

English

Title :

Determination of thyreostats in urine using supported liquid extraction and mixed-mode cation-exchange solid-phase extraction: Screening and confirmatory methods

Publication date :

2018

Journal title :

Journal of Chromatographic Science

ISSN :

0021-9665

eISSN :

1945-239X

Publisher :

Preston Publications, United States - Illinois

Volume :

Issue :

Pages :

858-866

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

NIEHS - National Institute of Environmental Health Sciences [US-NC] [US-NC]

Available on ORBi :

since 11 December 2021

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