Impact of rearing temperature on encapsulation and the accumulation of transcripts putatively involved in capsule formation in a parasitized lepidopteran host

Seehausen, M. L.; Naumann, Paul-Henri; Béliveau, C.; Martel, V.; Cusson, M.

doi:10.1016/j.jinsphys.2018.04.013

Article (Scientific journals)

Impact of rearing temperature on encapsulation and the accumulation of transcripts putatively involved in capsule formation in a parasitized lepidopteran host

Seehausen, M. L.; Naumann, Paul-Henri; Béliveau, C. et al.

2018 • In Journal of Insect Physiology, 107, p. 244-249

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/265873

DOI
10.1016/j.jinsphys.2018.04.013

PubMed
29704478

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Keywords :

Choristoneura fumiferana; Encapsulation; Endoparasitoid; Melanisation; Temperature; Tranosema rostrale; Hexapoda; Hymenoptera; Ichneumonidae; Lepidoptera; Tortricidae; Animals; Down-Regulation; Gene Expression Regulation; Host-Parasite Interactions; Hot Temperature; Insect Proteins; Larva; Moths; Up-Regulation

Abstract :

[en] Encapsulation and melanisation are innate immune reactions of insects against foreign intruders such as parasitoids. In an earlier study, we observed that immature life stages of the endoparasitoid Tranosema rostrale (Hymenoptera: Ichneumonidae) parasitizing Choristoneura fumiferana (Lepidoptera: Tortricidae) larvae experienced higher mortality due to encapsulation and melanisation when reared at high (30 °C) than at lower (10 °C, 20 °C) temperatures. Downregulation of T. rostrale polydnavirus genes in parasitized hosts and upregulation of two genes involved in the spruce budworm's melanisation process were identified as likely contributors to parasitoid mortality at high temperature. However, levels of transcripts of genes involved in the spruce budworm's cellular encapsulation process were not measured inasmuch as candidate genes, in the spruce budworm, had not yet been identified. In addition, our assessment of temperature-dependent encapsulation and melanisation of foreign objects in spruce budworm larvae was only partial. To fill these knowledge gaps, we injected Sephadex™ beads into unparasitized spruce budworm larvae and assessed their encapsulation/melanisation after the insects had been held at three different temperatures (10, 20, and 30 °C), and we identified spruce budworm genes putatively involved in the encapsulation process and quantified their transcripts at the same three temperatures, using a qPCR approach. As expected, both encapsulation and melanisation of Sephadex™ beads increased as a function of temperature. At the molecular level, three of the five genes examined (Integrin β1, Hopscotch, Stat92E) clearly displayed temperature-dependent upregulation. The results of this study further support the hypothesis that a temperature-dependent increase in the encapsulation response of C. fumiferana against T. rostrale is due to the combined effects of reduced expression of polydnavirus genes and enhanced expression of host immune genes. © 2018 Elsevier Ltd

Disciplines :

Anatomy (cytology, histology, embryology...) & physiology

Author, co-author :

Seehausen, M. L.; University of Toronto, Faculty of Forestry, 33 Willcocks Street, Toronto, Ontario M5S 3B3, Canada

Naumann, Paul-Henri ; Université de Liège - ULiège

Béliveau, C.; Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P.E.P.S., Quebec City, G1V 4C7, Canada

Martel, V.; Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P.E.P.S., Quebec City, G1V 4C7, Canada

Cusson, M.; Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P.E.P.S., Quebec City, G1V 4C7, Canada

Language :

English

Title :

Impact of rearing temperature on encapsulation and the accumulation of transcripts putatively involved in capsule formation in a parasitized lepidopteran host

Publication date :

2018

Journal title :

Journal of Insect Physiology

ISSN :

0022-1910

Publisher :

Elsevier, United Kingdom

Volume :

107

Pages :

244-249

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

ACOA - Atlantic Canada Opportunities Agency
CFS - Canadian Forest Service

Available on ORBi :

since 08 December 2021

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