Poster (Scientific congresses and symposiums)
Molecular origin of microcystins in dietary supplements originating from the Klamath Lake, Oregon
Van Hassel, Wannes; Ahn, Anne-Catherine; Huybrechts, Bart et al.
2021US-HAB
 

Files


Full Text
USHAB poster_Wannes Van Hassel.pdf
Author preprint (526.39 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Food Supplements; Toxic Cyanobacteria; Taxonomy
Abstract :
[en] Cyanobacteria can proliferate to high cell densities (blooms) in lakes, (artificial) ponds or closed-system cultures and are used as dietary supplements. Certain cyanobacterial species are also capable of producing toxins, for instance, hepatotoxins (e.g., microcystins, nodularin and cylindrospermopsin) or neurotoxins (e.g., anatoxin-a and saxitoxin). During a screening for eight microcystin congeners (MCs) and nodularin in cyanobacteria- and Chlorella-based dietary supplements sold on the Belgium market, nine products were found to be contaminated. These products should only contain harvested Aphanizomenon flos-aquae, a species that blooms in the Upper Klamath Lake (South Oregon, US)1. As it is not clear if this species can produce MCs, the possible occurrence of other species in the blooms could be responsible for the MCs presence. After quantifying the MCs with a validated LC-MS/MS method, the presence of genes involved in the production of MCs was tested. The mcyE gene was amplified using PCR and sequenced by the Sanger method. MCs concentrations ranged between 39.7 and 4837.4 µg/kg MC-LR equivalent. Two dietary supplements contained concentrations higher than 1 µg/kg MC-LR equivalent which is proposed as regulatory limit by Gilroy et al.2. The phylogenetic affiliation of the mcyE sequences could be traced back to Microcystis sp. for seven products. The contamination of the supplements might be explained by the presence of Microcystis during the harvest of A. flos-aquae dominated blooms, as already observed in the past2. 1. Carmichael, et al., J. Appl. Phycol. 12, 585–595 (2000). 2. Gilroy, et al., Environ. Health Perspect. 108, 435–439 (2000).
Research center :
Biological Sciences from Molecules to Systems - inBioS
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Van Hassel, Wannes  ;  Université de Liège - ULiège > InBioS
Ahn, Anne-Catherine ;  Université de Liège - ULiège > Département des sciences de la vie > Physiologie et génétique bactériennes
Huybrechts, Bart;  Sciensano > Organic contaminants and Additives > Toxines
Masquelier, Julien
Andjelkovic, Mirjana;  Sciensano > Risk and Health Impact Assessment
Wilmotte, Annick  ;  Université de Liège - ULiège > Département des sciences de la vie > Physiologie et génétique bactériennes
Language :
English
Title :
Molecular origin of microcystins in dietary supplements originating from the Klamath Lake, Oregon
Publication date :
May 2021
Event name :
US-HAB
Event organizer :
US National HAB committee
Event date :
25-05-2021 to 27-05-2021
Audience :
International
Name of the research project :
CyanoTox
Funders :
FAVV-AFSCA
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Available on ORBi :
since 07 December 2021

Statistics


Number of views
62 (4 by ULiège)
Number of downloads
1 (1 by ULiège)

Bibliography


Similar publications



Contact ORBi