Unpublished conference/Abstract (Scientific congresses and symposiums)
Technical and physiological testing of an ELISA kit for the determination of b-TSH in cattle: preliminary results
Eppe, Justine; PETROSSIANS, Patrick; Bossaert, Philippe et al.
2021FARAH Day 2021
 

Files


Full Text
Abstract FARAH DAY 2021 - KIT ELISA - J. EPPE.docx
Publisher postprint (17.6 kB)
Request a copy
Annexes
Presentation J. EPPE - FARAH 2021.pdf
Publisher postprint (657.14 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
cattle; metabolism; thyroid; iodine; diagnostic
Abstract :
[en] The thyroid gland plays an important role regarding health and production of cattle. Nevertheless, the metabolism and the functions of this gland remain poorly understood compared to other animal species. Moreover, diagnostic tools to assess the thyroid status in cattle are lacking while thyrotropin hormone is often used in other species. This study tested an ELISA kit for the determination of bovine thyrotropin (b-TSH) via a thyrotropin releasing hormone (TRH) stimulation test and a b-TSH inhibition test performed on 4 young Holstein calves. The ELISA kit had an intra-assay coefficient higher than the 9% announced in the kit notice (17.5 +/- 5.2%) and did not respond consistently to the inhibition test (differences between extreme values: p>0.1). Nevertheless, this kit seems sensitive to the stimulation test and showed significantly different (p<0.05) b-TSH concentrations when basal values (T-60, T-30, T0) are compared to T+5, T+10 and T+100 minutes after intravenous TRH injection. Further studies are needed to determine whether this ELISA kit can be used routinely. For example, the intra- and inter-assay coefficients could be calculated in the same way as in the kit notice or b-TSH could be measured on serum instead of plasma. In view of the involvement of thyroid metabolism in milk production or in the pathogenesis of different conditions (e.g. acute respiratory distress syndrome), b-TSH status could be assessed as an additional tool to diet analysis or as a prognosis factor.
Research center :
FARAH - Fundamental and Applied Research for Animals and Health - ULiège
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Eppe, Justine  ;  Université de Liège - ULiège > Dépt d'Ens. et de Clinique des animaux de Production (DCP) > Gestion de la santé des ruminants
PETROSSIANS, Patrick  ;  Centre Hospitalier Universitaire de Liège - CHU > Département de médecine interne > Service d'endocrinologie clinique
Bossaert, Philippe ;  Université de Liège - ULiège > Dépt d'Ens. et de Clinique des animaux de Production (DCP) > Gestion de la santé des ruminants
Rollin, Frédéric ;  Université de Liège - ULiège > Dépt d'Ens. et de Clinique des animaux de Production (DCP) > Gestion de la santé des ruminants
Guyot, Hugues  ;  Université de Liège - ULiège > Dépt d'Ens. et de Clinique des animaux de Production (DCP) > Gestion de la santé des ruminants
Language :
English
Title :
Technical and physiological testing of an ELISA kit for the determination of b-TSH in cattle: preliminary results
Publication date :
19 November 2021
Event name :
FARAH Day 2021
Event organizer :
FARAH
Event place :
Liège, Belgium
Event date :
Le 19 novembre 2021
Available on ORBi :
since 22 November 2021

Statistics


Number of views
68 (9 by ULiège)
Number of downloads
1 (1 by ULiège)

Bibliography


Similar publications



Contact ORBi