Article (Scientific journals)
The Evolution of the Cryopreservation Techniques in Reproductive Medicine—Exploring the Character of the Vitrified State Intra- and Extracellularly to Better Understand Cell Survival after Cryopreservation
Vanderzwalmen, Pierre; Ectors, Fabien; Panagiotidis, Yannis et al.
2020In Reproductive Medicine, 1, p. 142-157
Peer reviewed
 

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Keywords :
Cryopreservation; Cryobiology; Vitrification; slow freezing; Assisted Reproduction techniques (ART); Embryo; Gametes; Macromolecular crowding; Aqueous solidification; Colloidal solidification
Abstract :
[en] Nowadays, cryopreservation of gametes and embryos is a fundamental, integral, and indispensable part of infertility treatment or fertility preservation. Cryopreservation is not only needed for the policy of single embryo transfer and cryopreservation of surplus embryos, but for deferring embryo transfer in the case of ovarian hyperstimulation syndrome, uterine pathologies, and suboptimal endometrium built-up or when preimplantation genetic testing is needed. Several current strategies in assisted reproduction technology (ART) would be inconceivable without highly efficient cryopreservation protocols. Nevertheless, cryopreservation hampered for a long time, especially in terms of low survival rates after freezing and thawing. Only the technical progress during the last decades, namely, in regard to the implementation and advancement of vitrification, leveraged its application, and thus, even allows the cryopreservation of human oocytes—a process that is far from being easy. This review aims to provide a deeper insight into the physical processes of cryopreservation and to explore the character of the vitrified state in the extra and intracellular milieu in order to demonstrate that the common denominator to all cryopreservation procedures is the establishment of an intracellular amorphous condition that hinders the likelihood of crystallization.
Disciplines :
Reproductive medicine (gynecology, andrology, obstetrics)
Author, co-author :
Vanderzwalmen, Pierre
Ectors, Fabien  ;  Université de Liège - ULiège > Scientifiques attachés au Doyen (F MV)
Panagiotidis, Yannis
Schuf, Maximilian
Murtinger, Maximilian
Wirleitner, Barbara
Language :
English
Title :
The Evolution of the Cryopreservation Techniques in Reproductive Medicine—Exploring the Character of the Vitrified State Intra- and Extracellularly to Better Understand Cell Survival after Cryopreservation
Publication date :
17 September 2020
Journal title :
Reproductive Medicine
Publisher :
MDPI, Basel, Switzerland
Special issue title :
Embryo Technologies Management
Volume :
1
Pages :
142-157
Peer reviewed :
Peer reviewed
Available on ORBi :
since 15 October 2021

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