Phosphorus; Root system architecture; Rhizobacteria; PGPR; Phosphorus use efficiency; in vitro
Abstract :
[en] Mineral phosphorus (P) fertilisers must be used wisely in order to preserve rock phosphate, a limited and non-renewable resource. The use of bio-inoculants to improve soil nutrient availability and trigger an efficient plant response to nutrient deficiency is one potential strategy in the attempt to decrease P inputs in agriculture.
An in vitro co-cultivation system was used to study the response of Brachypodium distachyon to contrasted P supplies (soluble and poorly soluble forms of P) and inoculation with P solubilizing bacteria. Brachypodium's responses to P conditions and inoculation with bacteria were studied in terms of developmental plasticity and P use efficiency.
Brachypodium showed plasticity in its biomass allocation pattern in response to variable P conditions, specifically by prioritizing root development over shoot productivity under poorly soluble P conditions. Despite the ability of the bacteria to solubilize P, shoot productivity was depressed in plants inoculated with bacteria, although the root system development was maintained. The negative impact of bacteria on biomass production in Brachypodium might be attributed to inadequate C supply to bacteria, an increased competition for P between both organisms under P-limiting conditions, or an accumulation of toxic bacterial metabolites in our cultivation system. Both P and inoculation treatments impacted root system morphology. The modulation of Brachypodium’s developmental response to P supplies by P solubilizing bacteria did not lead to improved P use efficiency.
Our results support the hypothesis that plastic responses of Brachypodium cultivated under P-limited conditions are modulated by P solubilizing bacteria. The considered experimental context impacts plant–bacteria interactions. Choosing experimental conditions as close as possible to real ones is important in the selection of P solubilizing bacteria. Both persistent homology and allometric analyses proved to be useful tools that should be considered when studying the impact of bio-inoculants on plant development in response to varying nutritional context.
Delory, Benjamin; Leuphana University, Lüneburg, Germany > Institute of Ecology
Spaepen, Stijn; University of Leuven, Belgium > Leuven Institute for Beer Research
du Jardin, Patrick ; Université de Liège - ULiège > Département GxABT > Plant Sciences
Delaplace, Pierre ; Université de Liège - ULiège > Département GxABT > Plant Sciences
Language :
English
Title :
Developmental plasticity of Brachypodium distachyon in response to P deficiency: modulation by inoculation with phosphate-solubilizing bacteria
Alternative titles :
[fr] Plasticité developpementale de Brachypodium distachyon en réponse à la déficience en phosphore: modulation par inoculation de bactéries solubilisatrices du phosphate
We thank Assucopie, the Belgian society for the management of the collective rights of educational, academic,
scientific and university authors, for its grant to support the publication.
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