Global and local genetic diversity at two microsatellite loci in Plasmodium vivax parasites from Asia, Africa and South America

Schousboe, Mette L; Ranjitkar, Samir; Rajakaruna, Rupika S; Amerasinghe, Priyanie H; Konradsen, Flemming; Morales Yanes, Francisco Javier; Ord, Rosalynn; Rowland, Mark; Pearce, Richard; Leslie, Toby; Gadalla, , Nahla; Bygbjerg, Ib C; Alifrangis, Michael; Roper, Cally

doi:10.1186/1475-2875-13-392

Article (Scientific journals)

Global and local genetic diversity at two microsatellite loci in Plasmodium vivax parasites from Asia, Africa and South America

Schousboe, Mette L; Ranjitkar, Samir; Rajakaruna, Rupika S et al.

2014 • In Malaria Journal, 13, p. 392

Peer Reviewed verified by ORBi

Permalink
https://hdl.handle.net/2268/264205

DOI
10.1186/1475-2875-13-392

PubMed
25277367

Files (1)Send to Details Statistics Bibliography Similar publications

Files

Full Text

1475-2875-13-392.pdf

Publisher postprint (553.48 kB)

Download

All documents in ORBi are protected by a user license.

Send to

RIS BibTex APA Chicago Permalink X Linkedin

Details

Keywords :

Malaria; Genotyping; Diversity,; Heterozygosity; Plasmodium vivax; Microsatellite

Abstract :

[en] Background: Even though Plasmodium vivax has the widest worldwide distribution of the human malaria species and imposes a serious impact on global public health, the investigation of genetic diversity in this species has been limited in comparison to Plasmodium falciparum. Markers of genetic diversity are vital to the evaluation of drug and vaccine efficacy, tracking of P. vivax outbreaks, and assessing geographical differentiation between parasite populations. Methods: The genetic diversity of eight P. vivax populations (n = 543) was investigated by using two microsatellites (MS), m1501 and m3502, chosen because of their seven and eight base-pair (bp) repeat lengths, respectively. These were compared with published data of the same loci from six other P. vivax populations. Results: In total, 1,440 P. vivax samples from 14 countries on three continents were compared. There was highest heterozygosity within Asian populations, where expected heterozygosity (He) was 0.92-0.98, and alleles with a high repeat number were more common. Pairwise FST revealed significant differentiation between most P. vivax populations, with the highest divergence found between Asian and South American populations, yet the majority of the diversity (~89%) was found to exist within rather than between populations. Conclusions: The MS markers used were informative in both global and local P. vivax population comparisons and their seven and eight bp repeat length facilitated population comparison using data from independent studies. A complex spatial pattern of MS polymorphisms among global P. vivax populations was observed which has potential utility in future epidemiological studies of the P. vivax parasite.

Disciplines :

Immunology & infectious disease

Author, co-author :

Schousboe, Mette L; University of Copenhagen, Copenhagen, Denmark > Centre for Medical Parasitology, Institute of International Health, Immunology, and Microbiology

Ranjitkar, Samir; University Hospital, Copenhagen, Denmark > Department of Infectious Diseases, Copenhagen

Rajakaruna, Rupika S; University of Peradeniya, Peradeniya 20400, Sri Lanka. > Department of Zoology

Amerasinghe, Priyanie H; International Water Management Institute, Hyderabad, Andhra Pradesh, India

Konradsen, Flemming; University Hospital, Copenhagen, Denmark > Department of Infectious Diseases, Copenhagen

Morales Yanes, Francisco Javier ; Universidad San Francisco de Quito. Robles y Pampite, Cumbaya, Ecuador > Instituto de Microbiología

Ord, Rosalynn; London School of Hygiene and Tropical Medicine, Keppel Street, London WC1 4HT, UK. > Faculty of Infectious and Tropical Diseases

Rowland, Mark; London School of Hygiene and Tropical Medicine, Keppel Street, London WC1 4HT, UK. > Faculty of Infectious and Tropical Diseases

Pearce, Richard; London School of Hygiene and Tropical Medicine, Keppel Street, London WC1 4HT, UK. > Faculty of Infectious and Tropical Diseases

Leslie, Toby; London School of Hygiene and Tropical Medicine, Keppel Street, London WC1 4HT, UK. > Faculty of Infectious and Tropical Diseases

More authors (4 more)

Language :

English

Title :

Global and local genetic diversity at two microsatellite loci in Plasmodium vivax parasites from Asia, Africa and South America

Publication date :

October 2014

Journal title :

Malaria Journal

eISSN :

1475-2875

Publisher :

BioMed Central, United Kingdom

Volume :

Pages :

392

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 12 October 2021

Statistics

Number of views

50 (1 by ULiège)

Number of downloads

28 (1 by ULiège)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

OpenCitations

Bibliography

Neafsey DE, Galinsky K, Jiang RH, Young L, Sykes SM, Saif S, Gujja S, Goldberg JM, Young S, Zeng Q, Chapman SB, Dash AP, Anvikar AR, Sutton PL, Birren BW, Escalante AA, Barnwell JW, Carlton JM: The malaria parasite Plasmodium vivax exhibits greater genetic diversity than Plasmodium falciparum. Nat Genet 2012, 44:1046-1050.
Beg MA, Khan R, Baig SM, Gulzar Z, Hussain R, Smego RA Jr: Cerebral involvement in benign tertian malaria. Am J Trop Med Hyg 2002, 67:230-232.
Genton B, DAcremont V, Rare L, Baea K, Reeder JC, Alpers MP, Möller I: Plasmodium vivax and mixed infections are associated with severe malaria in children: A prospective cohort study from Papua New Guinea. PLoS Med 2008, 5:e127.
Singh H, Parakh A, Basu S, Rath B: Plasmodium vivax malaria: is it actually benign? J Infect Public Health 2011, 4:91-95.
Tjitra E, Anstey NM, Sugiarto P, Warikar N, Kenangalem E, Karyana M, Lampah DA, Price RN: Multidrug-resistant Plasmodium vivax associated with severe and fatal malaria: A prospective study in Papua, Indonesia. PLoS Med 2008, 5:e128.
Honma H, Kim JY, Palacpac NM, Mita T, Lee W, Horii T, Tanabe K: Recent increase of genetic diversity in Plasmodium vivax population in the Republic of Korea. Malar J 2011, 10:257.
Price RN, Douglas NM, Anstey NM: New developments in Plasmodium vivax malaria: severe disease and the rise of chloroquine resistance. Curr Opin Infect Dis 2009, 22:430-435.
Imwong M, Pukrittayakamee S, Renia L, Letourneur F, Charlieu JP, Leartsakulpanich U, Looareesuwan S, White NJ, Snounou G: Novel point mutations in the dihydrofolate reductase gene of Plasmodium vivax: evidence for sequential selection by drug pressure. Antimicrob Agents Chemother 2003, 47:1514-1521.
Cui L, Mascorro CN, Fan Q, Rzomp KA, Khuntirat B, Zhou G, Chen H, Yan G, Sattabongkot J: Genetic diversity and multiple infections of Plasmodium vivax malaria in Western Thailand. Am J Trop Med Hyg 2003, 68:613-619.
Schousboe ML, Rajakaruna RS, Amerasinghe PH, Konradsen F, Ord R, Pearce R, Bygbjerg IC, Roper C, Alifrangis M: Analysis of polymorphisms in the merozoite surface protein-3alpha gene and two microsatellite loci in Sri Lankan Plasmodium vivax: evidence of population substructure in Sri Lanka. Am J Trop Med Hyg 2011, 85:994-1001.
Kim JR, Imwong M, Nandy A, Chotivanich K, Nontprasert A, Tonomsing N, Maji A, Addy M, Day NP, White NJ, Pukrittayakamee S: Genetic diversity of Plasmodium vivax in Kolkata, India. Malar J 2006, 5:71.
Ord R, Polley S, Tami A, Sutherland CJ: High sequence diversity and evidence of balancing selection in the Pvmsp3alpha gene of Plasmodium vivax in the Venezuelan Amazon. Mol Biochem Parasitol 2005, 144:86-93.
Imwong M, Pukrittayakamee S, Gruner AC, Renia L, Letourneur F, Looareesuwan S, White NJ, Snounou G: Practical PCR genotyping protocols for Plasmodium vivax using Pvcs and Pvmsp1. Malar J 2005, 4:20.
Brito CF, Ferreira MU: Molecular markers and genetic diversity of Plasmodium vivax. Mem Inst Oswaldo Cruz 2011, 106(Suppl 1):12-26.
Russell B, Suwanarusk R, Lek-Uthai U: Plasmodium vivax genetic diversity: microsatellite length matters. Trends Parasitol 2006, 22:399-401.
Anderson TJ, Su XZ, Bockarie M, Lagog M, Day KP: Twelve microsatellite markers for characterization of Plasmodium falciparum from finger-prick blood samples. Parasitology 1999, 119(Pt 2):113-125.
Imwong M, Nair S, Pukrittayakamee S, Sudimack D, Williams JT, Mayxay M, Newton PN, Kim JR, Nandy A, Osorio L, Carlton JM, White NJ, Day NP, Anderson TJ: Contrasting genetic structure in Plasmodium vivax populations from Asia and South America. Int J Parasitol 2007, 37:1013-1022.
Koepfli C, Mueller I, Marfurt J, Goroti M, Sie A, Oa O, Genton B, Beck HP, Felger I: Evaluation of Plasmodium vivax genotyping markers for molecular monitoring in clinical trials. J Infect Dis 2009, 199:1074-1080.
Karunaweera ND, Ferreira MU, Munasinghe A, Barnwell JW, Collins WE, King CL, Kawamoto F, Hartl DL, Wirth DF: Extensive microsatellite diversity in the human malaria parasite Plasmodium vivax. Gene 2008, 410:105-112.
Gunawardena S, Karunaweera ND, Ferreira MU, Phone-Kyaw M, Pollack RJ, Alifrangis M, Rajakaruna RS, Konradsen F, Amerasinghe PH, Schousboe ML, Galappaththy GN, Abeyasinghe RR, Hartl DL, Wirth DF: Geographic structure of Plasmodium vivax: microsatellite analysis of parasite populations from Sri Lanka, Myanmar, and Ethiopia. Am J Trop Med Hyg 2010, 82:235-242.
Ellegren H: Microsatellites: simple sequences with complex evolution. Nat Rev Genet 2004, 5:435-445.
Imwong M, Sudimack D, Pukrittayakamee S, Osorio L, Carlton JM, Day NP, White NJ, Anderson TJ: Microsatellite variation, repeat array length, and population history of Plasmodium vivax. Mol Biol Evol 2006, 23:1016-1018.
Ranjitkar S, Schousboe ML, Thomsen TT, Adhikari M, Kapel CM, Bygbjerg IC, Alifrangis M: Prevalence of molecular markers of anti-malarial drug resistance in Plasmodium vivax and Plasmodium falciparum in two districts of Nepal. Malar J 2011, 10:75.
Leslie T, Mayan MI, Hasan MA, Safi MH, Klinkenberg E, Whitty CJ, Rowland M: Sulfadoxine-pyrimethamine, chlorproguanil-dapsone, or chloroquine for the treatment of Plasmodium vivax malaria in Afghanistan and Pakistan: A randomized controlled trial. JAMA 2007, 297:2201-2209.
Leslie T, Mayan I, Mohammed N, Erasmus P, Kolaczinski J, Whitty CJ, Rowland M: A randomised trial of an eight-week, once weekly primaquine regimen to prevent relapse of Plasmodium vivax in Northwest Frontier Province, Pakistan. PLoS One 2008, 3:e2861.
Ord RL, Tami A, Sutherland CJ: Ama1 genes of sympatric Plasmodium vivax and P. falciparum from Venezuela differ significantly in genetic diversity and recombination frequency. PLoS One 2008, 3:e3366.
Wooden J, Kyes S, Sibley CH: PCR and strain identification in Plasmodium falciparum. Parasitol Today 1993, 9:303-305.
Pearce RJ, Drakeley C, Chandramohan D, Mosha F, Roper C: Molecular determination of point mutation haplotypes in the dihydrofolate reductase and dihydropteroate synthase of Plasmodium falciparum in three districts of Northern Tanzania. Antimicrob Agents Chemother 2003, 47:1347-1354.
Goudet J: FSTAT, A Program to Estimate and Test Gene Diversities and Fixation Indices. [2.9.3]. ; 2001. Ref Type: Computer Program, http://www.unil.ch/izea/softwares/Fstat.html. Updated from Goudet (1995).
Arlekin: An Integrated Software for Population Genetics Data Analysis. [3.11]; 2012. Ref Type: Computer Program.
Google: Google Earth. 2014. 24-8-2014. Ref Type: Online Source.
Orjuela-Sanchez P, da Silva NS, da Silva-Nunes M, Ferreira MU: Recurrent parasitemias and population dynamics of Plasmodium vivax polymorphisms in rural Amazonia. Am J Trop Med Hyg 2009, 81:961-968.
Bruce MC, Galinski MR, Barnwell JW, Donnelly CA, Walmsley M, Alpers MP, Walliker D, Day KP: Genetic diversity and dynamics of Plasmodium falciparum and P. vivax populations in multiply infected children with asymptomatic malaria infections in Papua New Guinea. Parasitology 2000, 121(Pt 3):257-272.
Kolakovich KA, Ssengoba A, Wojcik K, Tsuboi T, Al-Yaman F, Alpers M, Adams JH: Plasmodium vivax: favored gene frequencies of the merozoite surface protein-1 and the multiplicity of infection in a malaria endemic region. Exp Parasitol 1996, 83:11-19.
Ferreira MU, Karunaweera ND, da Silva-Nunes M, da Silva NS, Wirth DF, Hartl DL: Population structure and transmission dynamics of Plasmodium vivax in rural Amazonia. J Infect Dis 2007, 195:1218-1226.
Cui L, Escalante AA, Imwong M, Snounou G: The genetic diversity of Plasmodium vivax populations. Trends Parasitol 2003, 19:220-226.