Article (Scientific journals)
Interlaboratory comparison of 25-hydroxyvitamin D assays: Vitamin D Standardization Program (VDSP) Intercomparison Study 2 - Part 1 liquid chromatography - tandem mass spectrometry (LC-MS/MS) assays - impact of 3-epi-25-hydroxyvitamin D(3) on assay performance.
Wise, Stephen A.; Camara, Johanna E.; Burdette, Carolyn Q. et al.
2022In Analytical and Bioanalytical Chemistry, 414 (1), p. 333-349
Peer Reviewed verified by ORBi
 

Files


Full Text
s00216-021-03576-1.pdf
Publisher postprint (3.41 MB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
24R,25-Dihydroxyvitamin D3 (24R,25(OH)3D3); 25-Hydroxyvitamin D2 (25(OH)D2); 25-Hydroxyvitamin D3 (25(OH)D3); 3-epi-25-Hydroxyvitamin D3 (3-epi-25(OH)D3); Liquid chromatography – tandem mass spectrometry (LC-MS/MS); Total serum 25-hydroxyvitamin D (25(OH)D)
Abstract :
[en] An interlaboratory comparison study was conducted by the Vitamin D Standardization Program (VDSP) to assess the performance of liquid chromatography - tandem mass spectrometry (LC-MS/MS) assays used for the determination of serum total 25-hydroxyvitamin D (25(OH)D), which is the sum of 25-hydroxyvitamin D(2) (25(OH)D(2)) and 25-hydroxyvitamin D(3) (25(OH)D(3)). A set of 50 single-donor samples was assigned target values for concentrations of 25(OH)D(2), 25(OH)D(3), 3-epi-25-hydroxyvitamin D(3) (3-epi-25(OH)D(3)), and 24R,25-dihydroxyvitamin D(3) (24R,25(OH)(2)D(3)) using isotope dilution liquid chromatography - tandem mass spectrometry (ID LC-MS/MS). VDSP Intercomparison Study 2 Part 1 includes results from 14 laboratories using 14 custom LC-MS/MS assays. Assay performance was evaluated using mean % bias compared to the assigned target values and using linear regression analysis of the test assay mean results and the target values. Only 53% of the LC-MS/MS assays met the VDSP criterion of mean % bias ≤ |±5%|. For the LC-MS/MS assays not meeting the ≤ |±5%| criterion, four assays had mean % bias of between 12 and 21%. Based on multivariable regression analysis using the concentrations of the four individual vitamin D metabolites in the 50 single-donor samples, the performance of several LC-MS/MS assays was found to be influenced by the presence of 3-epi-25(OH)D(3). The results of this interlaboratory study represent the most comprehensive comparison of LC-MS/MS assay performance for serum total 25(OH)D and document the significant impact of the lack of separation of 3-epi-25(OH)D(3) and 25(OH)D(3) on assay performance, particularly with regard to mean % bias.
Disciplines :
Laboratory medicine & medical technology
Author, co-author :
Wise, Stephen A.
Camara, Johanna E.
Burdette, Carolyn Q.
Hahm, Grace
Nalin, Federica
Kuszak, Adam J.
Merkel, Joyce
Durazo-Arvizu, Ramón A.
Williams, Emma L.
Hoofnagle, Andrew N.
Ivison, Fiona
Fischer, Ralf
van den Ouweland, Jody M. W.
Ho, Chung S.
Law, Emmett W. K.
Simard, Jean-Nicolas
Gonthier, Renaud
Holmquist, Brett
Meadows, Sarah
Cox, Lorna
Robyak, Kimberly
Creer, Michael H.
Fitzgerald, Robert
Clarke, Michael W.
Breen, Norma
LUKAS, Pierre  ;  Centre Hospitalier Universitaire de Liège - CHU > Unilab > Bone and cartilage markers laboratory
Cavalier, Etienne  ;  Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Sempos, Christopher T.
More authors (18 more) Less
Language :
English
Title :
Interlaboratory comparison of 25-hydroxyvitamin D assays: Vitamin D Standardization Program (VDSP) Intercomparison Study 2 - Part 1 liquid chromatography - tandem mass spectrometry (LC-MS/MS) assays - impact of 3-epi-25-hydroxyvitamin D(3) on assay performance.
Publication date :
January 2022
Journal title :
Analytical and Bioanalytical Chemistry
ISSN :
1618-2642
eISSN :
1618-2650
Publisher :
Springer, Germany
Volume :
414
Issue :
1
Pages :
333-349
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
© 2021. This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply.
Available on ORBi :
since 30 September 2021

Statistics


Number of views
112 (3 by ULiège)
Number of downloads
4 (3 by ULiège)

Scopus citations®
 
14
Scopus citations®
without self-citations
7
OpenCitations
 
7

Bibliography


Similar publications



Contact ORBi