tetracationic porphyrins; photo therapy; breast cacer
Abstract :
[en] Photodynamic therapy (PDT) is an alternative to conventional chemotherapy for the treatment of several types of cancer. Its advantages are reduced side effects and development of resistance mechanisms. In this work, we evaluated the photosensitization capabilities of 5,10,15,20-tetrakis[4-(pyridinium-1-yl-methyl)phenyl]porphyrin, its N-confused isomer and tow if its neutral precursors. The results were compared with the ones obtained with the cationic 5,10,15,20-tetrakis(1-methylpyridinium-4-yl)porphyrin (TMPyP) used as positive control. Both regular porphyrin derivatives showed higher efficiency to generate singlet oxygen than TMPyP. Next, we demonstrated that one of the cationic porphyrin is an efficient photosentitizer kills MCF7 breast cancer cells. The study of the cell death mechanisms induced by the photodynamic process showed that 5,10,15,20-tetrakis[4-(pyridinium-1-yl-methyl)phenyl]porphyrin and TMPyP caused cell death by autophagic flux and necrosis.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Gamelas, Sara; University of Aveiro > Deparment of Chemistry > LAQV-REQUIMTE
Moura, Nuno; University of Aveiro > Department of Chemistry > LAQV-REQUIMTE
Habraken, Yvette ; Université de Liège - ULiège > GIGA Molecul. Biolog. of Diseases - Gene Expression & Cancer
Piette, Jacques; University of Liege - ULiege > GIGA-MBD > Virology and Immunology
Neves, Maria; University of Aveiro > Department of Chemistry > LAQV-REQUIMTE
Faustino, Maria; University of Aveiro > Department of Chemistry > LAQV-REQUIMTE
Language :
English
Title :
Tetracationic porphyrin derivatives against human breast cancer
Alternative titles :
[en] Dérivés des Porphyrines Tétracationique dans le cancer du sein
Publication date :
September 2021
Journal title :
Journal of Photochemistry and Photobiology B: Biology
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