Spatio-temporal distribution of DMI and SDHI fungicide resistance of Zymoseptoria tritici throughout Europe based on frequencies of key target-site alterations

Hellin, Pierre; Duvivier, Maxime; Heick, Thies M.; Fraaije, Bart A.; Bataille, Charlotte; Clinckemaillie, Aurélie; Legrève, Anne; Jørgensen, Lise N.; Andersson, Björn; Samils, Berit; Rodemann, Bernd; Berg, Gunilla; Hutton, Fiona; Garnault, Maxime; El Jarroudi, Moussa; Couleaud, Gilles; Kildea, Steven

doi:10.1002/ps.6601

Article (Scientific journals)

Spatio-temporal distribution of DMI and SDHI fungicide resistance of Zymoseptoria tritici throughout Europe based on frequencies of key target-site alterations

Hellin, Pierre; Duvivier, Maxime; Heick, Thies M. et al.

2021 • In Pest Management Science

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/263688

DOI
10.1002/ps.6601

PubMed
34392616

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Keywords :

Mycosphaerella graminicola; sensitivity; disease control; septoria tritici blotch; triazole; wheat

Abstract :

[en] BACKGROUND: Over the past decade, demethylation inhibitor (DMI) and succinate dehydrogenase inhibitor (SDHI) fungicides have been extensively used to control to septoria tritici blotch, caused by Zymoseptoria tritici on wheat. This has led to the development and selection of alterations in the target-site enzymes (CYP51 and SDH, respectively). RESULTS: Taking advantage of newly and previously developed qPCR assays, the frequency of key alterations associated with DMI (CYP51-S524T) and SDHI (SDHC-T79N/I, C-N86S and C-H152R) resistance was assessed in Z. tritici-infected wheat leaf samples collected from commercial crops (n = 140) across 14 European countries prior to fungicide application in the spring of 2019. This revealed the presence of a West to East gradient in the frequencies of the most common key alterations conferring azole (S524T) and SDHI resistance (T79N and N86S), with the highest frequencies measured in Ireland and Great Britain. These observations were corroborated by sequencing (CYP51 and SDH subunits) and sensitivity phenotyping (prothioconazole-desthio and fluxapyroxad) of Z. tritici isolates collected from a selection of field samples. Additional sampling made at the end of the 2019 season confirmed the continued increase in frequency of the targeted alterations. Investigations on historical leaf DNA samples originating from different European countries revealed that the frequency of all key alterations (except C-T79I) has been gradually increasing over the past decade.

Disciplines :

Agriculture & agronomy

Author, co-author :

Hellin, Pierre

Duvivier, Maxime

Heick, Thies M.

Fraaije, Bart A.

Bataille, Charlotte

Clinckemaillie, Aurélie

Legrève, Anne

Jørgensen, Lise N.

Andersson, Björn

Samils, Berit

More authors (7 more)

Language :

English

Title :

Spatio-temporal distribution of DMI and SDHI fungicide resistance of Zymoseptoria tritici throughout Europe based on frequencies of key target-site alterations

Publication date :

21 August 2021

Journal title :

Pest Management Science

ISSN :

1526-498X

eISSN :

1526-4998

Publisher :

John Wiley & Sons, Hoboken, United States - New Jersey

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 22 September 2021

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