Development of routine RT-PCR ELOSA tests for fruit tree certification.
Publication date :
2001
Event name :
Proceedings of the 18th International Symposium on Virus & Virus-likeDiseases of Temperate Fruit Crops: Top Fruit Diseases, volume 1, EastMalling, Kent, UK, 9-15 July 2000.
Journal title :
Acta Horticulturae
ISSN :
0567-7572
eISSN :
2406-6168
Publisher :
International Society for Horticultural Science, Belgium
Bugos R.C., Chiang V.L., Zhang X.H., Campbell W.H., Podila G.K. and Campbell, W.R., 1995. RNA isolation from plant tissues recalcitrant to extraction in guanidine. Biotechniques 19: 734-737
Chandelier A., Cognet S., Marinho V.L.A., Kummert J. and Lepoivre, P., 1998. Development of routine detection tests using PCR for certification. Mededelingen van het Faculteit van de Landbouwwetenschappen van het Universiteit te Gent 63/4b: 1473-1978.
Kummert J., Marinho V.L.A., Rufflard G., Colinet D. and Lepoivre P., 1998. Sensitive detection of apple stem grooving and apple stem pitting viruses from infected apple trees by RT-PCR. Acta. Horticulturae 472: 97-104.
Marinho V.L.A., Kummert J., Rufflard G., Colinet D. and Lepoivre P., 1998. Detection of apple stem grooving virus in dormant apple trees by using crude extracts as templates for one-step-RT-PCR. Plant Diseases 82: 785-790.
Minsavage G.V., Thompson C.M., Hopkins D.L., Leite R.M.V.B. and Stall R.E., 1994. Development of a polymerase chain reaction protocol for detection of Xylella fastidiosa in plant tissue. Phytopathology 84: 456-461.
Rasmussen S.R., Larsen M.R. and Rasmussen S.E., 1991. Covalent immobilisation of DNA onto polystyrene microwells: the molecules are only bound at then 5' end. Anal. Biochem. 198: 138-142.
Rowhani A., Maningas M.A., Lile L.S., Daubert S.D. and Golino D.A., 1995. Development of a detection system for viruses of woody plants based on PCR analysis of immobilized virions. Phytopathology 85: 347-352.
Sambrook J., Fritsch E. and Maniatis T., 1989. Molecular cloning: A laboratory manual. New York, Cold Spring Harbor Laboratory.