Structural interactions define assembly adapter function of a type II secretion system pseudopilin.

Escobar, Cristian A.; Douzi, Badreddine; Ball, Geneviève; Barbat, Brice; Alphonse, Sebastien; Quinton, Loïc; Voulhoux, Romé; Forest, Katrina T.

doi:10.1016/j.str.2021.05.015

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Structural interactions define assembly adapter function of a type II secretion system pseudopilin.

Escobar, Cristian A.; Douzi, Badreddine; Ball, Geneviève et al.

2021 • In Structure

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https://hdl.handle.net/2268/262749

DOI
10.1016/j.str.2021.05.015

PubMed
34139172

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Keywords :

PDB-Dev; Pseudomonas aeruginosa; chemical-shift perturbation; cross-linking mass spectrometry; pilin; pseudopilin; type II secretion; type IV pili

Abstract :

[en] The type IV filament superfamily comprises widespread membrane-associated polymers in prokaryotes. The type II secretion system (T2SS), a virulence pathway in many pathogens, belongs to this superfamily. A knowledge gap in understanding of the T2SS is the molecular role of a small "pseudopilin" protein. Using multiple biophysical techniques, we have deciphered how this missing component of the Xcp T2SS architecture is structurally integrated, and thereby unlocked its function. We demonstrate that low-abundance XcpH is the adapter that bridges a trimeric initiating tip complex, XcpIJK, with a periplasmic filament of XcpG subunits. Each pseudopilin protein caps an XcpG protofilament in an overall pseudopilus compatible with dimensions of the periplasm and the outer membrane-spanning secretin through which substrates pass. Unexpectedly, to fulfill its adapter function, the XcpH N-terminal helix must be unwound, a property shared with XcpG subunits. We provide an experimentally validated three-dimensional structural model of a complete type IV filament.

Disciplines :

Chemistry

Author, co-author :

Escobar, Cristian A.

Douzi, Badreddine

Ball, Geneviève

Barbat, Brice

Alphonse, Sebastien

Quinton, Loïc ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie biologique

Voulhoux, Romé

Forest, Katrina T.

Language :

English

Title :

Structural interactions define assembly adapter function of a type II secretion system pseudopilin.

Publication date :

2021

Journal title :

Structure

ISSN :

0969-2126

eISSN :

1878-4186

Peer reviewed :

Peer reviewed

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Available on ORBi :

since 25 August 2021

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