[en] Several phytoplankton species produce the metabolites dimethylsulfoniopropionate (DMSP) and dimethylsulfoxide (DMSO) but their intracellular roles need to be better understood. To improve the understanding of the DMSP antioxidant function suggested by Sunda et al. (2002), we exposed the diatom Skeletonema costatum, the Prymnesiophyceae Phaeocystis globosa and the dinoflagellate Heterocapsa triquetra to experimental treatments known to cause potential oxidative stress (high light intensities (HL); HL with 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU); menadione sodium bisulfite (MSB)). DMSP and DMSO concentrations decreased after 6h in all treatments indicating an interaction with Reactive Oxygen Species (ROS) produced. DMSP and DMSO-to-cell ratios in control conditions were higher for H. triquetra, while being unable to grow under HL. DMSP and DMSO-to-cell carbon were the highest for P. globosa while the other species had similar values. During long-term treatment, these ratios were not increased in high-light grown cells of P. globosa and S. costatum. Overall, this illustrates that (1) the DMSP- and DMSO-to-cell or carbon seems to be not indicative of the capability of the species to tolerate an oxidative stress, (2) these molecules could react with ROS and lower their cellular concentration, but no clues demonstrated that these molecules are part of the antioxidant response of the cell.
Research Center/Unit :
FOCUS - Freshwater and OCeanic science Unit of reSearch - ULiège InBios - Integrative Biological Sciences - ULiège
Alcolombri, U., Ben-Dor, S., Feldmesser, E., Levin, Y., Tawfik, D. S. and Vardi, A. (2015) Identification of the algal dimethyl sulfidereleasing enzyme: A missing link in the marine sulfur cycle. Science, 348, 1466?1469.
Apel, K. and Hirt, H. (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu. Rev. Plant Biol., 55, 373?399.
Archer, S. D., Stefels, J., Airs, R. L., Lawson, T., Smyth, T. J., Rees, A. P. and Geider, R. J. (2018) Limitation of dimethylsulfoniopropionate synthesis at high irradiance in natural phytoplankton communities of the tropical Atlantic. Limnol. Oceanogr., 63, 227?242.
Asada, K. (2006) Production and scavenging of reactive oxygen species in chloroplasts and their functions. Plant Physiol., 141, 391?396.
Ayers, G. P. and Cainey, J. M. (2007) The CLAW hypothesis: A review of the major developments. Environ. Chem., 4, 366.
Baker, N. R. (2008) Chlorophyll fluorescence: A probe of photosynthesis in vivo. Annu. Rev. Plant Biol., 59, 89?113.
Bell, T. G., Malin, G., Kim, Y.-N. and Steinke, M. (2007) Spatial variability in DMSP-lyase activity along an Atlantic meridional transect. Aquat. Sci., 69, 320?329.
Borges, A. A., Dobon, A., Exposito-Rodriguez, M., Jimenez-Arias, D., Borges-Perez, A., Casanas-Sanchez, V., Perez, J. A., Luis, J. C. et al. (2009) Molecular analysis of menadione-induced resistance against biotic stress in Arabidopsis. Plant Biotechnol. J., 7, 744?762.
Borges, A. V. and Champenois, W. (2017) Preservation protocol for dimethylsulfoniopropionate and dimethylsulfoxide analysis in plant material of the Mediterranean seagrass Posidonia oceanica , and reevaluation of dimethylsulfoniopropionate leaf content. Aquat. Bot., 143, 8?10.
Borisova-Mubarakshina,M.M., Ivanov, B. N., Vetoshkina, D. V., Lubimov, V. Y., Fedorchuk, T. P., Naydov, I. A.,Kozuleva, M. A., Rudenko, N. N. et al. (2015) Long-Termacclimatory response to excess excitation energy: evidence for a role of hydrogen peroxide in the regulation of photosystem II antenna size. EXBOTJ, 66, 7151?7164.
Brunet, C., Johnsen, G., Lavaud, J. & Roy, S. (2011) Pigments and Photoacclimation processes. in Phytoplankton Pigments (eds. Roy, S., Llewellyn, C., Egeland, E. S. & Johnsen, G.) 445?471 (Cambridge University Press, Cambridge). https://doi.org/10.1017/CB O9780511732263.017.
Bucciarelli, E., Ridame, C., Sunda, W. G., Dimier-Hugueney, C., Cheize, M. and Belviso, S. (2013) Increased intracellular concentrations of DMSP and DMSO in iron-limited oceanic phytoplankton Thalassiosira oceanica and Trichodesmium erythraeum. Limnol. Oceanogr., 58, 1667?1679.
Bucciarelli, E. and Sunda, W. G. (2003) Influence of CO2, nitrate, phosphate, and silicate limitation on intracellular dimethylsulfoniopropionate in batch cultures of the coastal diatom Thalassiosira pseudonana. Limnol. Oceanogr., 48, 2256?2265.
Caruana, A. M. N. and Malin, G. (2014) The variability in DMSP content and DMSP lyase activity in marine dinoflagellates. Prog. Oceanogr., 120, 410?424.
Caruana, A. M. N. (2010) DMS and DMSP production by marine dinoflagellates. Archimer, archive institutionnelle de l?Ifremer, ID: 10670/1.ijom5j.
Champenois, W. and Borges, A. V. (2019) Determination of dimethylsulfoniopropionate and dimethylsulfoxide in Posidonia oceanica leaf tissue. MethodsX, 6, 56?62.
Charlson, R. J., Lovelock, J. E., Andreae, M. O. and Warren, S. G. (1987) Oceanic phytoplankton, atmospheric Sulphur, cloud albedo and climate. Nature, 326, 655?661.
Curran, M. A., Jones, G. B., and Burton, H. (1998) Spatial distribution of dimethylsulfide and dimethylsulfoniopropionate in the Australasian sector of the Southern Ocean. J. Geophys., 103, 16667?16689.
Curson, A. R. J.,Williams, B. T., Pinchbeck, B. J., Sims, L. P.,Martinez, A. B., Rivera, P. P. L., Kumaresan, D., Mercade, E. et al. (2018) DSYB catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton. Nat. Microbiol., 3, 430?439.
Dacey, J. W. H. and Blough, N. V. (1987) Hydroxide decomposition of dimethylsulfoniopropionate to formdimethylsulfide. Geophys. Res. Lett., 14, 1246?1249.
Darroch, L., Lavoie, M., Levasseur, M., Laurion, I., Sunda, W. G., Michaud, S., Scarratt, M., Gosselin, M. et al. (2015) Effect of shortterm light-and UV-stress on DMSP, DMS, and DMSP lyase activity in Emiliania huxleyi. Aquat. Microb. Ecol., 74, 173?185.
del Valle, D. A., Slezak, D., Smith, C. M., Rellinger, A. N., Kieber, D. J. and Kiene, R. P. (2011) Effect of acidification on preservation of DMSP in seawater and phytoplankton cultures: evidence for rapid loss and cleavage of DMSP in samples containing Phaeocystis sp. Mar. Chem., 124, 57?67.
Deschaseaux, E. S. M., Jones, G. B., Deseo, M. A., Shepherd, K. M., Kiene, R. P., Swan, H. B., Harrison, P. L. and Eyre, B. D. (2014) Effects of environmental factors on dimethylated sulfur compounds and their potential role in the antioxidant system of the coral holobiont. Limnol. Oceanogr., 59, 758?768.
Diaz, J. M. and Plummer, S. (2018) Production of extracellular reactive oxygen species by phytoplankton: past and future directions. J. Plankton Res., 40, 655?666. https://doi.org/10.1093/plankt/fby039.
Dummermuth, A. L., Karsten, U., Fisch, K. M., Konig, G. M. and Wiencke, C. (2003) Responses of marine macroalgae to hydrogenperoxide stress. J. Exp. Mar. Biol. Ecol., 289, 103?121
Erickson, E., Wakao, S. and Niyogi, K. K. (2015) Light stress and photoprotection in Chlamydomonas reinhardtii. Plant J., 82, 449?465.
Farmer, E. E. and Mueller, M. J. (2013) ROS-mediated lipid peroxidation and RES-Activated Signaling. Annu. Rev. Plant Biol., 64, 429?450.
Fischer, B. B., Ledford, H. K., Wakao, S., Huang, S. G., Casero, D., Pellegrini, M., Merchant, S. S., Koller, A. et al. (2012) SINGLET OXYGEN RESISTANT 1 links reactive electrophile signaling to singlet oxygen acclimation in Chlamydomonas reinhardtii. Proc. Natl. Acad. Sci., 109, E1302?E1311.
Fuse, H., Takimura, O., Kamimura, K., Murakami, K., Yamaoka, Y. and Murooka, Y. (1995) Transformation of dimethyl Sulfide and related compounds by cultures and cell extracts of marine phytoplankton. Biosci. Biotechnol. Biochem., 59, 1773?1775.
Gardner, S. G., Nielsen, D. A., Laczka, O. F., Shimmon, R., Beltran, V. H., Ralph, P. and Petrou, K. (2016) Dimethylsulfoniopropionate, superoxide dismutase and glutathione as stress response indicators in three corals under short-Term hyposalinity stress. Proc. R. Soc. Lond. B Biol. Sci., 283, 20152418.
Genty, B., Briantais, J.-M. and Baker, N. R. (1989) The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. BBA-Gen. Subjects, 990, 87?92.
Goiris, K., van Colen, W., Wilches, I., Leon-Tamariz, F., de Cooman, L. and Muylaert, K. (2015) Impact of nutrient stress on antioxidant production in three species of microalgae. Algal Res., 7, 51?57.
Goss, R. and Jakob, T. (2010) Regulation and function of xanthophyll cycle-dependent photoprotection in algae. Photosynth. Res., 106, 103?122.
Goss-Sampson, M. A., van Doorn, J. and Wagenmakers, E. J. (2020) Bayesian inference in JASP: A guide for students. University of Amsterdam: JASP team.
Guillard, R. R. L. and Ryther, J. H. (1962) Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt and Detonula confervacea Cleve. Can. J. Microbiol., 8, 229?239.
Halliwell, B. (1995) Antioxidant characterization, methodology and mechanism. Biochem. Pharmacol., 49, 1341?1348.
Harada, H., Rouse, M.-A., Sunda, W. and Kiene, R. P. (2004) Latitudinal and vertical distributions of particle-Associated dimethylsulfoniopropionate (DMSP) lyase activity in the western North Atlantic Ocean. Can. J. Fish. Aquat. Sci., 61, 700?711.
Hasle, G. R. (1978) The Inverted Microscope Method. Phytoplankton Manual, UNESCO, Paris, p. 8896.
Hassan, H. M. and Fridovich, I. (1979) Intracellular production of superoxide radical and of hydrogen peroxide by redox active compounds. Arch. Biochem. Biophys., 196, 385?395.
Hatton, A. D. and Wilson, S. T. (2007) Particulate dimethylsulphoxide and dimethylsulphoniopropionate in phytoplankton cultures and Scottish coastal waters. Aquat. Sci., 69, 330?340.
Haynes, D., Ralph, P., Prange, J. and Dennison, B. (2000) The impact of the herbicide Diuron on photosynthesis in three species of tropical seagrass. Mar. Pollut. Bull., 41, 288?293.
Hillebrand, H., Durselen, C.-D., Kirschtel, D., Pollingher, U. and Zohary, T. (1999) Biovolume calculation for pelagic and benthic microalgae. J. Phycol., 35, 403?424.
Husband, J. D., Kiene, R. P. and Sherman, T. D. (2012) Oxidation of dimethylsulfoniopropionate (DMSP) in response to oxidative stress in Spartina alterniflora and protection of a non-DMSP producing grass by exogenous DMSP+acrylate. Environ. Exp. Bot., 79, 44?48.
Jahns, P. and Holzwarth, A. R. (2012) The role of the xanthophyll cycle and of lutein in photoprotection of photosystem II. Biochim. Biophys. Acta. Bioenerg., 1817, 182?193.
Karsten, U., Kirst, G. O. and Wiencke, C. (1992) Dimethylsulphoniopropionate (DMSP) accumulation in green macioalgae from polar to temperate regions: interactive effects of light versus salinity and light versus temperature. Polar Biol., 12, 603?607.
Keller, M. D. and Korjeff-Bellows, W. (1996) Physiological aspects of the production of dimeyhtlsulfoniopropionate (DMSP) by marine phytoplankton. In Biological and environmental chemistry of DMSP and related sulfonium compounds. Springer, Boston, MA, 131?142.
Keller, M. D., Bellows, W. K. and Guillard, R. R. L. (1989) Dimethyl sulfide production in marine phytoplankton. In Saltzman, E. S. and Cooper,W. J. (eds.), Biogenic sulfur in the environment, Vol. 393, American Chemical Society, Washington DC, pp. 167?182.
Kiene, R. P. and Gerard, G. (1994) Determination of trace levels of dimethylsulfoxide (DMSO) in seawater and rainwater. Mar. Chem., 47, 1?12.
Kiene, R. P. and Slezak, D. (2006) Low dissolved DMSP concentrations in seawater revealed by small volume gravity filtration and dialysis sampling. Limnol. Oceanogr. Methods, 4, 80?95.
Kinsey, J. D. and Kieber, D. J. (2016) Microwave preservation method for DMSP, DMSO, and acrylate in unfiltered seawater and phytoplankton culture samples. Limnol. Oceanogr. Methods, 14, 196?209.
Kirst, G. O. (1996) Osmotic adjustment in phytoplankton and macroalgae. In Biological and environmental chemistry of DMSP and related sulfonium compounds, Springer, Boston, MA, 121?129.
Lavoie, M., Levasseur, M. and Babin, M. (2015) Testing the potential ballast role for dimethylsulfoniopropionate in marine phytoplankton: A modeling study. J. Plankton Res., 37, 699?711.
Lee, P. A. and De Mora, S. J. (1999) Intracellular Dimethylsulfoxide (DMSO) in unicellular marine algae: speculations on its origin and possible biological role. J. Phycol., 35, 8?18. https://doi.org/10.1046/j.1529-8817.1999.3510008.x.
Lesser, M. P. (2006) Oxidative stress in marine environments: biochemistry and physiological ecology. Annu. Rev. Physiol., 68, 253?278.
Liss, P. S., Hatton, A. D., Malin, G., Nightingale, P. D. and Turner, S. M. (1997) Marine Sulphur emissions. Phil. Trans. R. Soc. Lond. B, 352, 159?169.
Litchman, E. and Klausmeier, C. A. (2001) Competition of phytoplankton under fluctuating light. Am. Nat., 157, 170?187.
Lund, J. W. G., Kipling, C. and Le Cren, E. D. (1958) The inverted microscope method of estimating algal numbers and the statistical basis of estimations by counting. Hydrobiologia, 11, 143?170.
Mallick, N. and Mohn, F. H. (2000) Reactive oxygen species: response of algal cells. J. Plant Physiol., 157, 183?193.
McParland, E. L. and Levine, N. M. (2019) The role of differential DMSP production and community composition in predicting variability of global surface DMSP concentrations. Limnol. Oceanogr., 64, 757?773.
Menden-Deuer, S. and Lessard, E. J. (2000) Carbon to volume relationships for dinoflagellates, diatoms, and other protist plankton. Limnol. Oceanogr., 45, 569?579.
Menden-Deuer, S., Lessard, E. and Satterberg, J. (2001) Effect of preservation on dinoflagellate and diatom cell volume, and consequences for carbon biomass predictions. Mar. Ecol. Prog. Ser., 222, 41?50.
Mohapatra, B. R., Rellinger, A. N., Kieber, D. J. and Kiene, R. P. (2014) Kinetics of DMSP lyases in whole cell extracts of four Phaeocystis species: response to temperature and DMSP analogs. J. Sea Res., 86, 110?115.
Moller, I. M., Jensen, P. E. and Hansson, A. (2007) Oxidative modifications to cellular components in plants. Annu. Rev. Plant Biol., 58, 459?481.
Muller, P., Li, X.-P. and Niyogi, K. K. (2001) Non-photochemical quenching. A response to excess light energy. Plant Physiol., 125, 1558?1566.
Murata, N., Takahashi, S., Nishiyama, Y. and Allakhverdiev, S. I. (2007) Photoinhibition of photosystem II under environmental stress. Biochim. Biophys. Acta. Bioenerg., 1767, 414?421.
Niki, T., Kunugi, M. and Otsuki, A. (2000) DMSP-lyase activity in five marine phytoplankton species: its potential importance in DMS production. Mar. Biol., 136, 759?764.
Nymark, M.,Valle, K. C., Brembu,T., Hancke, K.,Winge, P., Andresen, K., Johnsen, G. and Bones, A. M. (2009) An integrated analysis of molecular acclimation to high light in the marine diatom Phaeodactylum tricornutum. PLoS One, 4, e7743.
Pietta, P.-G. (2000) Flavonoids as antioxidants. J. Nat. Prod., 63, 1035?1042.
Pospisil, P. (2016) Production of reactive oxygen species by photosystem II as a response to light and temperature stress. Front. Plant Sci., 7, 1950.
Raina, J. B., Tapiolas,D.M., Foret, S., Lutz,A., Abrego,D., Ceh, J. et al. (2013) DMSP biosynthesis by an animal and its role in coral thermal stress response. Nature, 502, 677?680.
Raina, J.-B., Clode, P. L., Cheong, S., Bougoure, J., Kilburn, M. R., Reeder, A., Foret, S., Stat, M. et al. (2017) Subcellular tracking reveals the location of dimethylsulfoniopropionate in microalgae and visualises its uptake by marine bacteria. Elife, 6, e23008.
Ritchie, R. J. (2006) Consistent sets of spectrophotometric chlorophyll equations for acetone, methanol and ethanol solvents. Photosynth. Res., 89, 27?41.
Roberty, S., Fransolet, D., Cardol, P., Plumier, J.-C. and Franck, F. (2015) Imbalance between oxygen photoreduction and antioxidant capacities in Symbiodinium cells exposed to combined heat and high light stress. Coral Reefs, 34, 1063?1073.
Roberty, S., Furla, P. and Plumier, J.-C. (2016) Differential antioxidant response between two Symbiodinium species from contrasting environments: Antioxidant responses of Symbiodinium sp. Plant Cell Environ., 39, 2713?2724.
Ruban, A. V., Johnson, M. P. and Duffy, C. D. P. (2012) The photoprotective molecular switch in the photosystem II antenna. Biochim. Biophys. Acta. Bioenerg., 1817, 167?181.
Saragosti, E., Tchernov, D., Katsir, A. and Shaked, Y. (2010) Extracellular production and degradation of superoxide in the coral Stylophora pistillata and cultured Symbiodinium. PLoS One, 5, e12508.
Scaduto, R. (1995) Oxidation of DMSO and methanesulfinic acid by the hydroxyl radical. Free Radic. Biol. Med., 18, 271?277.
Seymour, J. R., Simoo, R., Ahmed, T. and Stocker, R. (2010) Chemoattraction to Dimethylsulfoniopropionate throughout the marine microbial food web. Science, 329, 342?345.
Sharma, P., Jha, A. B., Dubey, R. S. and Pessarakli, M. (2012) Reactive oxygen species, oxidative damage, and Antioxidative Defense mechanism in plants under stressful conditions. J. Bot., 2012, 1?26.
Simo, R. and Vila-Costa,M. (2006) Ubiquity of algal dimethylsulfoxide in the surface ocean: geographic and temporal distribution patterns. Mar. Chem., 100, 136?146.
Simo, R., Hatton, A., Malin, G. and Liss, P. (1998) Particulate dimethyl sulphoxide in seawater: production by microplankton. Mar. Ecol. Prog. Ser., 167, 291?296.
Smerilli, A., Balzano, S.,Maselli,M., Blasio,M., Orefice, I.,Galasso,C., Sansone, C. and Brunet, C. (2019) Antioxidant and Photoprotection networking in the coastal diatom Skeletonema marinoi. Antioxidants, 8, 154.
Speeckaert, G., Borges, A. V. and Gypens, N. (2019) Salinity and growth effects on dimethylsulfoniopropionate (DMSP) and dimethylsulfoxide (DMSO) cell quotas of Skeletonema costatum, Phaeocystis globosa and Heterocapsa triquetra. Estuar. Coast. Shelf Sci., 226, 106275.
Speeckaert, G., Borges, A. V., Champenois, W., Royer, C. and Gypens, N. (2018) Annual cycle of dimethylsulfoniopropionate (DMSP) and dimethylsulfoxide (DMSO) related to phytoplankton succession in the Southern North Sea. Sci. Total Environ., 622?623, 362?372.
Spielmeyer, A., Gebser, B. and Pohnert, G. (2011) Investigations of the uptake of Dimethylsulfoniopropionate by phytoplankton. Chem-BioChem, 12, 2276?2279.
Spiese, C. E., Kieber, D. J., Nomura, C. T. and Kiene, R. P. (2009) Reduction of dimethylsulfoxide to dimethylsulfide by marine phytoplankton. Limnol. Oceanogr., 54, 560?570.
Spiese, C. and Tatarkov, E. (2014) Dimethylsulfoxide reduction activity is linked to nutrient stress in Thalassiosira pseudonana NCMA 1335. Mar. Ecol. Prog. Ser., 507, 31?38.
Stefels, J. and Dijkhuizen, L. (1996) Characteristics of DMSP-lyase in Phaeocystis sp. (Prymnesiophyceae). Mar. Ecol. Prog. Ser., 131, 307?313.
Stefels, J. and VAN Leeuwe, M. A. (1998) Effects of iron and light stress on the biochemical composition of antarctic Phaeocystis sp. (Prymnesiophyceae). I. Intracellular DMSP concentrations. J. Phycol., 34, 486?495.
Stefels, J. (2009) Determination of DMS, DMSP, and DMSO in Seawater. In Wurl, O. (ed.), Practical Guidelines for the Analysis of Seawater, CRC Press, pp. 223?234. https://research.rug.nl/en/publications/ determination-of-dms-dmsp-And-dmso-in-seawater.
Stefels, J. (2000) Physiological aspects of the production and conversion of DMSP in marine algae and higher plants. J. Sea Res., 43, 183?197.
Stefels, J., Steinke, M., Turner, S., Malin, G. and Belviso, S. (2007) Environmental constraints on the production and removal of the climatically active gas dimethylsulphide (DMS) and implications for ecosystem modelling. Biogeochemistry, 83, 245?275.
Stefels, J., Dacey, J. W., and Elzenga, J. T. M. (2009) In vivo DMSP-biosynthesismeasurements using stable-isotope incorporation and proton-Transfer-reaction mass spectrometry (PTR-MS). Limnol. Oceanogr-meth., 7, 595?611.
Steinke, M., Malin, G., Gibb, S. W. and Burkill, P. H. (2002) Vertical and temporal variability of DMSP lyase activity in a coccolithophorid bloom in the northern North Sea. Deep Sea Res 2 Top Stud Oceanogr, 49, 3001?3016.
Strickland, J. D. H. and Parsons, T. R. (1972) A practical handbook of seawater analysis. A Pract. Handb. seawater Anal., 167, 185.
Strom, S., Wolfe, G., Holmes, J., Stecher, H., Shimeneck, C. and Sarah, L. (2003) Chemical defense in the microplankton I: feeding and growth rates of heterotrophic protists on the DMS-producing phytoplankterEmiliania huxleyi. Limnol. Oceanogr., 48, 217?229.
Sun, Y.-L., Zhao, Y., Hong, X. and Zhai, Z.-H. (1999) Cytochrome c release and caspase activation during menadione-induced apoptosis in plants. FEBS Lett., 462, 317?321.
Sunda, W. G., Hardison, R., Kiene, R. P., Bucciarelli, E. and Harada, H. (2007) The effect of nitrogen limitation on cellular DMSP and DMSrelease in marine phytoplankton: climate feedback implications. Aquat. Sci., 69, 341?351.
Sunda, W., Kieber, D. J., Kiene, R. P. and Huntsman, S. (2002) An antioxidant function for DMSP and DMS in marine algae. Nature, 418, 317?320.
Thume, K., Gebser, B., Chen, L.,Meyer, N., Kieber, D. J. and Pohnert, G. (2018) The metabolite dimethylsulfoxonium propionate extends the marine organosulfur cycle. Nature, 563, 412?415.
Trossat, C.,Rathinasabapathi, B.,Weretilnyk, E. A., Shen,T. L.,Huang, Z. H., Gage, D. A. and Hanson, A. D. (1998) Salinity promotes accumulation of 3-Dimethylsulfoniopropionate and its precursor SMethylmethionine in chloroplasts. Plant Physiol., 116, 165?171.
Van Alstyne,K. L. (2008) Ecological and physiological roles of dimethylsulfoniopropionate and its products in marine macroalgae. In Algal Chemical Ecology, Springer, Berlin, Heidelberg, 173?194.
van Doorn, J., van den Bergh, D., Bohm, U., Dablander, F., Derks, K., Draws, T. et al. (2021) The JASP guidelines for conducting and reporting a Bayesian analysis. Psychon. Bull. Rev., 28, 813?826.
Vega de Luna, F., Dang, K.-V., Cardol, M., Roberty, S. and Cardol, P. (2019) Photosynthetic capacity of the endosymbiotic dinoflagellate Cladocopium sp. is preserved during digestion of its jellyfish host Mastigias papua by the anemone Entacmaea medusivora. FEMS Microbiol. Ecol., 95, fiz141.
Wilkinson, F., Helman, W. P. and Ross, A. B. (1995) Rate constants for the decay and reactions of the lowest electronically excited singlet state of molecular oxygen in solution. An expanded and revised compilation. J. Phys. Chem. Ref. Data Monogr., 24, 663?677.
Wolfe, G. V., Steinke, M. and Kirst, G. O. (1997) Grazing-Activated chemical defence in a unicellular marine alga. Nature, 387, 894?897
