Comparison of the quantitative DiaSorin Liaison antigen test to RT-PCR for the diagnosis of COVID-19 in symptomatic and asymptomatic outpatients.

Lefever, Stephanie; Indevuyst, Christophe; Cuypers, Lize; Dewaele, Klaas; Yin, Nicolas; Cotton, Frédéric; Padalko, Elizaveta; Oyaert, Matthijs; Descy, Julie; Cavalier, Etienne; Van Ranst, Marc; André, Emmanuel; Lagrou, Katrien; Vermeersch, Pieter

doi:10.1128/JCM.00374-21

Article (Scientific journals)

Comparison of the quantitative DiaSorin Liaison antigen test to RT-PCR for the diagnosis of COVID-19 in symptomatic and asymptomatic outpatients.

Lefever, Stephanie; Indevuyst, Christophe; Cuypers, Lize et al.

2021 • In Journal of Clinical Microbiology

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https://hdl.handle.net/2268/262286

DOI
10.1128/JCM.00374-21

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33849953

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Keywords :

Covid-19; diagnosis; Covid

Abstract :

[en] BACKGROUND: We evaluated the quantitative DiaSorin Liaison SARS-CoV-2 antigen test in symptomatic and asymptomatic individuals consulting their general practioner (GP) during a period of stable intense virus circulation (213/100,000 habitants per day). METHODS: Left-over RT-PCR positive (n=204) and negative (n=210) nasopharyngeal samples were randomly selected among fresh routine samples collected from patients consulting their GP. Samples were tested on Liaison XL according to the manufacturer's instructions. Equivocal results were considered positive. RESULTS: Overall sensitivity and specificity of the Liaison antigen test compared to RT-PCR were 67.7% [95% confidence interval (CI): 60.9%-73.7%] and 100% [CI: 97.8%-100%]. Sensitivity in samples with a viral load ≥10(5), ≥10(4) and ≥10(3) copies/mL was 100% [CI: 96.3%-100.0%], 96.5% [CI: 91.8%-98.7%] and 87.4% [CI: 81.3%-91.5%], respectively. All samples ≤10(3) copies/mL were antigen negative. The ratio of antigen concentration to viral load in samples ≥10(3) copies/mL was comparable in symptomatic and asymptomatic individuals (p=0.58). The proportion of RT-PCR positive participants with a high viral load (≥10(5) copies/mL) was not significantly higher in symptomatic than in asymptomatic participants (63.9% [CI: 54.9%-72.0%] vs. 51.9% [CI: 41.1%-62.6%], p=0.11), but the proportion of participants with a low viral load (<10(3) copies/mL) was significantly higher in asymptomatic than in symptomatic RT-PCR positive participants (35.4% [CI: 25.8%-46.4%] vs. 14.3% [CI: 9.0%-21.8%], p<0.01). CONCLUSIONS: Sensitivity and specificity in samples with a viral load ≥10(4) copies/mL was 96.5% and 100%. The correlation of antigen concentration with viral load was comparable in symptomatic and asymptomatic individuals.

Disciplines :

Immunology & infectious disease
Laboratory medicine & medical technology

Author, co-author :

Lefever, Stephanie

Indevuyst, Christophe

Cuypers, Lize

Dewaele, Klaas

Yin, Nicolas

Cotton, Frédéric

Padalko, Elizaveta

Oyaert, Matthijs

Descy, Julie ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Bact., mycologie, parasitologie, virologie, microbio.

Cavalier, Etienne ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale

More authors (4 more)

Language :

English

Title :

Comparison of the quantitative DiaSorin Liaison antigen test to RT-PCR for the diagnosis of COVID-19 in symptomatic and asymptomatic outpatients.

Publication date :

April 2021

Journal title :

Journal of Clinical Microbiology

ISSN :

0095-1137

eISSN :

1098-660X

Publisher :

American Society for Microbiology, Washington, United States - District of Columbia

Peer reviewed :

Peer Reviewed verified by ORBi

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Available on ORBi :

since 26 July 2021

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