Article (Scientific journals)
Assessment of serum total 25-hydroxyvitamin D assay commutability of Standard Reference Materials and College of American Pathologists Accuracy-Based Vitamin D (ABVD) Scheme and Vitamin D External Quality Assessment Scheme (DEQAS) materials: Vitamin D Standardization Program (VDSP) Commutability Study 2.
Camara, Johanna E.; Wise, Stephen A.; Hoofnagle, Andrew N. et al.
2021In Analytical and Bioanalytical Chemistry, 413 (20), p. 5067-5084
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Keywords :
25-Hydroxyvitamin D2; 25-Hydroxyvitamin D3; Ligand binding assay; Liquid chromatography-tandem mass spectrometry (LC-MS/MS); Total 25-hydroxyvitamin D; Vitamin D Standardization Program (VDSP)
Abstract :
[en] An interlaboratory study was conducted through the Vitamin D Standardization Program (VDSP) to assess commutability of Standard Reference Materials® (SRMs) and proficiency testing/external quality assessment (PT/EQA) samples for determination of serum total 25-hydroxyvitamin D [25(OH)D] using ligand binding assays and liquid chromatography-tandem mass spectrometry (LC-MS/MS). A set of 50 single-donor serum samples were assigned target values for 25-hydroxyvitamin D(2) [25(OH)D(2)] and 25-hydroxyvitamin D(3) [25(OH)D(3)] using reference measurement procedures (RMPs). SRM and PT/EQA samples evaluated included SRM 972a (four levels), SRM 2973, six College of American Pathologists (CAP) Accuracy-Based Vitamin D (ABVD) samples, and nine Vitamin D External Quality Assessment Scheme (DEQAS) samples. Results were received from 28 different laboratories using 20 ligand binding assays and 14 LC-MS/MS methods. Using the test assay results for total serum 25(OH)D (i.e., the sum of 25(OH)D(2) and 25(OH)D(3)) determined for the single-donor samples and the RMP target values, the linear regression and 95% prediction intervals (PIs) were calculated. Using a subset of 42 samples that had concentrations of 25(OH)D(2) below 30 nmol/L, one or more of the SRM and PT/EQA samples with high concentrations of 25(OH)D(2) were deemed non-commutable using 5 of 11 unique ligand binding assays. SRM 972a (level 4), which has high exogenous concentration of 3-epi-25(OH)D(3), was deemed non-commutable for 50% of the LC-MS/MS assays.
Disciplines :
Laboratory medicine & medical technology
Author, co-author :
Camara, Johanna E.
Wise, Stephen A.
Hoofnagle, Andrew N.
Williams, Emma L.
Carter, Graham D.
Jones, Julia
Burdette, Carolyn Q.
Hahm, Grace
Nalin, Federica
Kuszak, Adam J.
Merkel, Joyce
Durazo-Arvizu, Ramón A.
LUKAS, Pierre  ;  Centre Hospitalier Universitaire de Liège - CHU > Unilab > Bone and cartilage markers laboratory
Cavalier, Etienne  ;  Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Popp, Christian
Beckert, Christian
Schultess, Jan
Van Slooten, Glen
Tourneur, Carole
Pease, Camille
Kaul, Ravi
Villarreal, Alfredo
Ivison, Fiona
Fischer, Ralf
van den Ouweland, Jody M. W.
Ho, Chung S.
Law, Emmett W. K.
Simard, Jean-Nicolas
Gonthier, Renaud
Holmquist, Brett
Batista, Marcelo Cidade
Pham, Heather
Bennett, Alex
Meadows, Sarah
Cox, Lorna
Jansen, Eugene
Khan, Dilshad Ahmed
Robyak, Kimberly
Creer, Michael H.
Kilbane, Mark
Twomey, Patrick J.
Freeman, James
Parker, Neil
Yuan, Jinyun
Fitzgerald, Robert
Mushtaq, Sohail
Clarke, Michael W.
Breen, Norma
Simpson, Christine
Sempos, Christopher T.
More authors (40 more) Less
Language :
English
Title :
Assessment of serum total 25-hydroxyvitamin D assay commutability of Standard Reference Materials and College of American Pathologists Accuracy-Based Vitamin D (ABVD) Scheme and Vitamin D External Quality Assessment Scheme (DEQAS) materials: Vitamin D Standardization Program (VDSP) Commutability Study 2.
Publication date :
August 2021
Journal title :
Analytical and Bioanalytical Chemistry
ISSN :
1618-2642
eISSN :
1618-2650
Publisher :
Springer, Germany
Volume :
413
Issue :
20
Pages :
5067-5084.
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 26 July 2021

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