Reference : Genome-wide association mapping of QTLs implied in potato virus Y population sizes in...
Scientific journals : Article
Life sciences : Phytobiology (plant sciences, forestry, mycology...)
Genome-wide association mapping of QTLs implied in potato virus Y population sizes in pepper: evidence for widespread resistance QTL pyramiding
Tamisier, lucie mailto [Université de Liège - ULg]
Szadkowski, M. [GAFL, INRA, Montfavet, 84140, France, Pathologie Végétale, INRA, Montfavet, 84140, France]
Nemouchi, G. [GAFL, INRA, Montfavet, 84140, France]
Lefebvre, V. [GAFL, INRA, Montfavet, 84140, France]
Szadkowski, E. [GAFL, INRA, Montfavet, 84140, France]
Duboscq, R. [GAFL, INRA, Montfavet, 84140, France]
Santoni, S. [UMR AGAP, INRA, Montpellier, F-34060, France]
Sarah, G. [UMR AGAP, INRA, Montpellier, F-34060, France]
Sauvage, C. [GAFL, INRA, Montfavet, 84140, France]
Palloix, A. [GAFL, INRA, Montfavet, 84140, France]
Moury, B. [Pathologie Végétale, INRA, Montfavet, 84140, France]
Molecular Plant Pathology
Yes (verified by ORBi)
United Kingdom
[en] Capsicum annuum ; Potato virus Y ; Potyvirus ; Capsicum ; Chromosome Mapping ; Chromosomes, Plant ; Disease Resistance ; Eukaryotic Initiation Factor-4E ; Genome-Wide Association Study ; Genotype ; Plant Diseases ; Polymorphism, Single Nucleotide ; Potyvirus ; Quantitative Trait Loci
[en] In this study, we looked for genetic factors in the pepper (Capsicum annuum) germplasm that control the number of potato virus Y (PVY) particles entering the plant (i.e. effective population size at inoculation) and the PVY accumulation at the systemic level (i.e. census population size). Using genotyping-by-sequencing (GBS) in a core collection of 256 pepper accessions, we obtained 10 307 single nucleotide polymorphisms (SNPs) covering the whole genome. Genome-wide association studies (GWAS) detected seven SNPs significantly associated with the virus population size at inoculation and/or systemic level on chromosomes 4, 6, 9 and 12. Two SNPs on chromosome 4 associated with both PVY population sizes map closely to the major resistance gene pvr2 encoding the eukaryotic initiation factor 4E. No obvious candidates for resistance were identified in the confidence intervals for the other chromosomes. SNPs detected on chromosomes 6 and 12 colocalized with resistance quantitative trait loci (QTLs) previously identified with a biparental population. These results show the efficiency of GBS and GWAS in C. annuum, indicate highly consistent results between GWAS and classical QTL mapping, and suggest that resistance QTLs identified with a biparental population are representative of a much larger collection of pepper accessions. Moreover, the resistance alleles at these different loci were more frequently combined than expected by chance in the core collection, indicating widespread pyramiding of resistance QTLs and widespread combination of resistance QTLs and major effect genes. Such pyramiding may increase resistance efficiency and/or durability. © 2019 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd
2019‐2022Institut National de la Recherche Agronomique, INRA
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

File(s) associated to this reference

Fulltext file(s):

Open access
mpp.12874.pdfPublisher postprint1.02 MBView/Open

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.