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Article (Scientific journals)
Genome-wide association mapping of QTLs implied in potato virus Y population sizes in pepper: evidence for widespread resistance QTL pyramiding
Tamisier, lucie  ; Szadkowski, M.; Nemouchi, G. et al.
2020 • In Molecular Plant Pathology, 21 (1), p. 3-16
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Keywords :
Capsicum annuum; Potato virus Y; Potyvirus; Capsicum; Chromosome Mapping; Chromosomes, Plant; Disease Resistance; Eukaryotic Initiation Factor-4E; Genome-Wide Association Study; Genotype; Plant Diseases; Polymorphism, Single Nucleotide; Quantitative Trait Loci
Abstract :
[en] In this study, we looked for genetic factors in the pepper (Capsicum annuum) germplasm that control the number of potato virus Y (PVY) particles entering the plant (i.e. effective population size at inoculation) and the PVY accumulation at the systemic level (i.e. census population size). Using genotyping-by-sequencing (GBS) in a core collection of 256 pepper accessions, we obtained 10 307 single nucleotide polymorphisms (SNPs) covering the whole genome. Genome-wide association studies (GWAS) detected seven SNPs significantly associated with the virus population size at inoculation and/or systemic level on chromosomes 4, 6, 9 and 12. Two SNPs on chromosome 4 associated with both PVY population sizes map closely to the major resistance gene pvr2 encoding the eukaryotic initiation factor 4E. No obvious candidates for resistance were identified in the confidence intervals for the other chromosomes. SNPs detected on chromosomes 6 and 12 colocalized with resistance quantitative trait loci (QTLs) previously identified with a biparental population. These results show the efficiency of GBS and GWAS in C. annuum, indicate highly consistent results between GWAS and classical QTL mapping, and suggest that resistance QTLs identified with a biparental population are representative of a much larger collection of pepper accessions. Moreover, the resistance alleles at these different loci were more frequently combined than expected by chance in the core collection, indicating widespread pyramiding of resistance QTLs and widespread combination of resistance QTLs and major effect genes. Such pyramiding may increase resistance efficiency and/or durability. © 2019 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd
Disciplines :
Phytobiology (plant sciences, forestry, mycology...)
Author, co-author :
Tamisier, lucie ;  Université de Liège - ULg
Szadkowski, M.;  GAFL, INRA, Montfavet, 84140, France, Pathologie Végétale, INRA, Montfavet, 84140, France
Nemouchi, G.;  GAFL, INRA, Montfavet, 84140, France
Lefebvre, V.;  GAFL, INRA, Montfavet, 84140, France
Szadkowski, E.;  GAFL, INRA, Montfavet, 84140, France
Duboscq, R.;  GAFL, INRA, Montfavet, 84140, France
Santoni, S.;  UMR AGAP, INRA, Montpellier, F-34060, France
Sarah, G.;  UMR AGAP, INRA, Montpellier, F-34060, France
Sauvage, C.;  GAFL, INRA, Montfavet, 84140, France
Palloix, A.;  GAFL, INRA, Montfavet, 84140, France
Moury, B.;  Pathologie Végétale, INRA, Montfavet, 84140, France
Language :
English
Title :
Genome-wide association mapping of QTLs implied in potato virus Y population sizes in pepper: evidence for widespread resistance QTL pyramiding
Publication date :
2020
Journal title :
Molecular Plant Pathology
ISSN :
1464-6722
eISSN :
1364-3703
Publisher :
Blackwell, Oxford, United Kingdom
Volume :
21
Issue :
1
Pages :
3-16
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
2019‐2022Institut National de la Recherche Agronomique, INRA

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