Article (Scientific journals)
Interactions between Kluyveromyces marxianus from cheese origin and the intestinal symbiont Bacteroides thetaiotaomicron: Impressive antioxidative effects
Ceugniez, A.; Tourret, M.; Dussert, E. et al.
2017In LWT - Food Science and Technology, 81, p. 281-290
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Keywords :
Anaerobic; Antioxidant; Bacteroides thetaiotaomicron; Kluyveromyces marxianus; Probiotic; Electrophoresis; Enzymes; Mass spectrometry; Oxygen; Yeast; Antioxidant properties; Antioxidative effects; Probiotics; Reactive oxygen species; Two-dimensional polyacrylamide gel electrophoresis; Antioxidants
Abstract :
[en] The effects of yeast Kluyveromyces marxianus S-2-05, of cheese origin, were assessed on the intestine anaerobe symbiont Bacteroides thetaiotaomicron ATCC 29741 to unveil any changes in its antioxidant properties. To this end, these microorganisms were grown and incubated either separately, or co-incubated, under anaerobic atmosphere. Afterwards, the microbial cells were recovered and washed, and extracts were prepared using a sterile detergent solution to mimic the intestine detergent content. The extracts prepared from K. marxianus S-2-05 and reference strain K. marxianus MUCL 29917, grown under different conditions, were assessed for their antioxidant properties against superoxide anion and hydrogen peroxide. Extracts from both yeasts showed antioxidative effects, which were particularly important for K. marxianus S-02-5 after anaerobic incubation. Moreover, K. marxianus S-02-5 displayed a high level of activity against the aforementioned reactive oxygen species, enhancing that of B. thetaiotaomicron ATCC 29741, after the co-incubation process. Two-dimensional polyacrylamide gel electrophoresis was used to separate the proteins extracted. Superoxide dismutase, thiol peroxidase, rubrerythrin -intensively produced by B. thetaiotaomicron induced by the yeast-were identified by mass spectrometry. The antioxidative potential evidenced for K. marxianus S-02-5 is another advantage which could justify the utilization of this strain as a probiotic for countering intestinal inflammatory processes. © 2017 Elsevier Ltd
Disciplines :
Biotechnology
Author, co-author :
Ceugniez, A.;  Univ. Lille, INRA, ISA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394–ICV - Institut Charles Viollette, Lille, France
Tourret, M.;  Univ. Lille, INRA, ISA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394–ICV - Institut Charles Viollette, Lille, France
Dussert, E.;  Univ. Lille, INRA, ISA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394–ICV - Institut Charles Viollette, Lille, France
Coucheney, F.;  Univ. Lille, INRA, ISA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394–ICV - Institut Charles Viollette, Lille, France
Deracinois, B.;  Univ. Lille, INRA, ISA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394–ICV - Institut Charles Viollette, Lille, France
Jacques, Philippe ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies
Flahaut, C.;  Univ. Lille, INRA, ISA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394–ICV - Institut Charles Viollette, Lille, France
Heuson, E.;  Univ. Lille, INRA, ISA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394–ICV - Institut Charles Viollette, Lille, France
Drider, D.;  Univ. Lille, INRA, ISA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394–ICV - Institut Charles Viollette, Lille, France
Behra-Miellet, J.;  Univ. Lille, INRA, ISA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394–ICV - Institut Charles Viollette, Lille, France
Language :
English
Title :
Interactions between Kluyveromyces marxianus from cheese origin and the intestinal symbiont Bacteroides thetaiotaomicron: Impressive antioxidative effects
Publication date :
2017
Journal title :
LWT - Food Science and Technology
ISSN :
0023-6438
eISSN :
1096-1127
Publisher :
Academic Press
Volume :
81
Pages :
281-290
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
ANR-11-EQPX-0037, ANR, Agence Nationale de la Recherche; MESRI, Ministère de l'Enseignement supérieur, de la Recherche  et de l'Innovation; FEDER, Federación Española de Enfermedades Raras
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