Article (Scientific journals)
Polynucleotide phosphorylase is involved in the control of lipopeptide fengycin production in Bacillus subtilis
Yaseen, Y.; Diop, A.; Gancel, F. et al.
2018In Archives of Microbiology, 200 (5), p. 783-791
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Keywords :
B. subtilis; Fengycin; Polynucleotide phosphorylase; Surfactin
Abstract :
[en] Bacillus subtilis is a wealth source of lipopeptide molecules such as iturins, surfactins and fengycins or plipastatins endowed with a range of biological activities. These molecules, designated secondary metabolites, are synthesized via non-ribosomal peptides synthesis (NRPS) machinery and are most often subjected to a complex regulation with involvement of several regulatory factors. To gain novel insights on mechanism regulating fengycin production, we investigated the effect of the fascinating polynucleotide phosphorylase (PNPase), as well as the effect of lipopeptide surfactin. Compared to the wild type, the production of fengycin in the mutant strains B. subtilis BBG235 and BBG236 altered for PNPase has not only decreased to about 70 and 40%, respectively, but also hampered its antifungal activity towards the plant pathogen Botrytis cinerea. On the other hand, mutant strains BBG231 (srfAA−) and BBG232 (srfAC−) displayed different levels of fengycin production. BBG231 had registered an important decrease in fengycin production, comparable to that observed for BBG235 or BBG236. This study permitted to establish that the products of pnpA gene (PNPase), and srfAA− (surfactin synthetase) are involved in fengycin production. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.
Disciplines :
Biotechnology
Microbiology
Author, co-author :
Yaseen, Y.;  Université de Lille, INRA, Université d’Artois, Université du Littoral-Côte d’Opale, EA 7394 – ICV-Institut Charles Viollette, Lille, France
Diop, A.;  Université de Lille, INRA, Université d’Artois, Université du Littoral-Côte d’Opale, EA 7394 – ICV-Institut Charles Viollette, Lille, France
Gancel, F.;  Université de Lille, INRA, Université d’Artois, Université du Littoral-Côte d’Opale, EA 7394 – ICV-Institut Charles Viollette, Lille, France
Béchet, M.;  Université de Lille, INRA, Université d’Artois, Université du Littoral-Côte d’Opale, EA 7394 – ICV-Institut Charles Viollette, Lille, France
Jacques, Philippe ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies
Drider, D.;  Université de Lille, INRA, Université d’Artois, Université du Littoral-Côte d’Opale, EA 7394 – ICV-Institut Charles Viollette, Lille, France
Language :
English
Title :
Polynucleotide phosphorylase is involved in the control of lipopeptide fengycin production in Bacillus subtilis
Publication date :
2018
Journal title :
Archives of Microbiology
ISSN :
0302-8933
eISSN :
1432-072X
Publisher :
Springer Verlag
Volume :
200
Issue :
5
Pages :
783-791
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 06 July 2021

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