Article (Scientific journals)
New Continuous Process for the Production of Lipopeptide Biosurfactants in Foam Overflowing Bioreactor
Guez, J.-S.; Vassaux, A.; Larroche, C. et al.
2021In Frontiers in Bioengineering and Biotechnology, 9
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Keywords :
Bacillus subtilis; Biomass; Biomolecules; Bioreactors; Surface active agents; Biomass concentrations; Lipopeptide biosurfactants; Overproducing strains; Situ product removal; Specific productivity; Stirred tank reactors; Theoretical modeling; Strain
Abstract :
[en] In this work, an original culture process in bioreactor named overflowing continuous culture (O-CC) was developed to produce and recover continuously mycosubtilin, a lipopeptide antifungal biosurfactant of major interest. The lipopeptide production was first investigated in shake conical flasks in different culture media [ammonium citrate sucrose (ACS), Difco sporulation medium (DSM), and Landy], followed by a pH condition optimization using 3-(N-morpholino)propanesulfonic acid (MOPS) and 2-(N-morpholino)ethanesulfonic acid (MES) buffered media. A simple theoretical modeling of the biomass evolution combined with an experimental setup was then proposed for O-CC processed in stirred tank reactor at laboratory scale. Seven O-CC experiments were done in modified Landy medium at the optimized pH 6.5 by applying dilution rates comprised between 0.05 and 0.1 h–1. The O-CC allowed the continuous recovery of the mycosubtilin contained in the foam overflowing out of the reactor, achieving a remarkable in situ product removal superior to 99%. The biomass concentration in the overflowing foam was found to be twofold lower than the biomass concentration in the reactor, relating advantageously this process to a continuous one with biomass feedback. To evaluate its performances regarding the type of lipopeptide produced, the O-CC process was tested with strain BBG116, a mycosubtilin constitutive overproducing strain that also produces surfactin, and strain BBG125, its derivative strain obtained by deleting surfactin synthetase operon. At a dilution rate of 0.1 h–1, specific productivity of 1.18 mg of mycosubtilin⋅g–1(DW)⋅h–1 was reached. Compared with other previously described bioprocesses using almost similar culture conditions and strains, the O-CC one allowed an increase of the mycosubtilin production rate by 2.06-fold. © Copyright © 2021 Guez, Vassaux, Larroche, Jacques and Coutte.
Disciplines :
Biotechnology
Microbiology
Author, co-author :
Guez, J.-S.;  Université Clermont Auvergne, Clermont Auvergne INP, CNRS, Institut Pascal, Clermont-Ferrand, France
Vassaux, A.;  Université de Lille, UMRt BioEcoAgro 1158-INRAE, équipe Métabolites Secondaires d’origine Microbienne, Institut Charles Viollette, Lille, France
Larroche, C.;  Université Clermont Auvergne, Clermont Auvergne INP, CNRS, Institut Pascal, Clermont-Ferrand, France
Jacques, Philippe ;  Université de Liège - ULiège > Département GxABT > Microbial, food and biobased technologies
Coutte, F.;  Université de Lille, UMRt BioEcoAgro 1158-INRAE, équipe Métabolites Secondaires d’origine Microbienne, Institut Charles Viollette, Lille, France, Lipofabrik, Polytech-Lille, Cité Scientifique, Villeneuve d’Ascq, France
Language :
English
Title :
New Continuous Process for the Production of Lipopeptide Biosurfactants in Foam Overflowing Bioreactor
Publication date :
2021
Journal title :
Frontiers in Bioengineering and Biotechnology
eISSN :
2296-4185
Publisher :
Frontiers Media S.A.
Volume :
9
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
Région Hauts-de-France [FR]
EU - European Union [BE]
Funding text :
H2020 - 849713 - Lipofabarik - A ground-breaking biomolecular production platform for safer, more efficient and sustainable pest control and crop health management
Available on ORBi :
since 06 July 2021

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