[en] Using the CRISPR/Cas9 technology, we
targeted the zebrafish col10a1a gene to
insert the Gfp coding sequence into its
genome, generating a transgenic zebrafish
line expressing a secreted Gfp fusion
protein in the developing skeleton that
reveals the bone extracellular matrix.
Research Center/Unit :
GIGA-I3 - Giga-Infection, Immunity and Inflammation - ULiège
Disciplines :
Genetics & genetic processes
Author, co-author :
Muller, Marc ; Université de Liège - ULiège > Département des sciences de la vie > GIGA I3 - Laboratory for Organogenesis and Regeneration
Raman, Ratish ; Université de Liège - ULiège > GIGA I3 - Laboratory for Organogenesis and Regeneration
Guerrero Limon, Gustavo ; Université de Liège - ULiège > GIGA I3 - Laboratory for Organogenesis and Regeneration
Renn, Joerg; Université de Liège - ULiège > GIGA I3 > Laboratory for Organogenesis and Regeneration > Post-Doc
Language :
English
Title :
A reporter zebrafish line for live fluorescent visualisation of bone extracellular matrix
Publication date :
23 September 2020
Event name :
The International CRISPR and Gene Editing Symposium
Event organizer :
rrogers@liebertpub.com;
Event place :
New York, United States
Event date :
23-09-2020
Audience :
International
European Projects :
H2020 - 766347 - BioMedaqu - Aquaculture meets Biomedicine: Innovation in Skeletal Health research.
Funders :
EU - European Union EC - European Commission
Commentary :
We present a transgenic zebrafish zebrafish line expressing a secreted Gfp fusion
protein in the developing skeleton that reveals the bone extracellular matrix.
