Putative Biomarkers for Malignant Pleural Mesothelioma Suggested by Proteomic Analysis of Cell Secretome

[en] BACKGROUND: Malignant pleural mesothelioma (MPM) a rare neoplasm linked to asbestos exposure is characterized by a poor prognosis. Soluble mesothelin is currently considered the most specific diagnostic biomarker. The aim of the study was to identify novel biomarkers by proteomic analysis of two MPM cell lines secretome. MATERIALS AND METHODS: The protein patterns of MPM cells secretome were examined and compared to a non-malignant mesothelial cell line using two-dimensional gel electrophoresis coupled to mass spectrometry. Serum levels of candidate biomarkers were determined in MPM patients and control subjects. RESULTS: Two up-regulated proteins involved in cancer biology, prosaposin and quiescin Q6 sulfhydryl oxidase 1, were considered candidate biomarkers. Serum levels of both proteins were significantly higher in MPM patients than control subjects. Combining the data of each receiver-operating characteristic analysis predicted a good diagnostic accuracy. CONCLUSION: A panel of the putative biomarkers represents a promising tool for MPM diagnosis. Copyright© 2020, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

Disciplines :

Cardiovascular & respiratory systems
Oncology

Author, co-author :

Lacerenza, S.; Department of Pharmacy, University of Pisa, Pisa, Italy

Ciregia, Federica ; Université de Liège - ULiège > I3-Rheumatology

Giusti, L.; School of Pharmacy, University of Camerino, Camerino, Italy, Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy

Bonotti, A.; Department of Translational Research and New Medical and Surgical Technologies, Occupational Medicine Unit, University-Hospital of Pisa, Pisa, Italy

Greco, V.; Institute of Biochemistry and Clinical Chemistry, Università Cattolica del Sacro Cuore, Rome, Italy, Fondazione Policlinico Universitario A. Gemelli IRCCS, Rome, Italy

Giannaccini, G.; Department of Pharmacy, University of Pisa, Pisa, Italy

D'Antongiovanni, V.; Department of Pharmacy, University of Pisa, Pisa, Italy

Fallahi, P.; Department of Translational Research and New Medical and Surgical Technologies, Occupational Medicine Unit, University-Hospital of Pisa, Pisa, Italy

Pieroni, L.; Proteomics and Metabonomics Unit, IRCCS-Fondazione Santa Lucia, Rome, Italy

Cristaudo, A.; Department of Translational Research and New Medical and Surgical Technologies, Occupational Medicine Unit, University-Hospital of Pisa, Pisa, Italy

More authors (3 more)

Language :

English

Title :

Putative Biomarkers for Malignant Pleural Mesothelioma Suggested by Proteomic Analysis of Cell Secretome

Publication date :

2020

Journal title :

Cancer genomics & proteomics

ISSN :

1790-6245

Publisher :

NLM (Medline)

Volume :

Issue :

Pages :

225-236

Peer reviewed :

Peer reviewed

Available on ORBi :

since 17 June 2021

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