Article (Scientific journals)
Intravitreal injection of anti-miRs against miR-142-3p reduces angiogenesis and microglia activation in a mouse model of laser-induced choroidal neovascularization.
Roblain, Quentin; Louis, Thomas; Yip, Cassandre et al.
2021In Aging, 13
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Keywords :
age-related macular degeneration; angiogenesis; inflammation; miR-142-3p; microglia
Abstract :
[en] Age-related macular degeneration (AMD) is a worldwide leading cause of blindness affecting individuals over 50 years old. The most aggressive form, wet AMD, is characterized by choroidal neovascularization (CNV) and inflammation involving microglia recruitment. By using a laser-induced CNV mouse model, we provide evidence for a key role played by miR-142-3p during CNV formation. MiR-142-3p was overexpressed in murine CNV lesions and its pharmacological inhibition decreased vascular and microglia densities by 46% and 30%, respectively. Consistently, miR-142-3p overexpression with mimics resulted in an increase of 136% and 126% of blood vessels and microglia recruitment. Interestingly, miR-142-3p expression was linked to the activation state of mouse microglia cells as determined by morphological analysis (cell solidity) through a computational method. In vitro, miR-142-3p overexpression in human microglia cells (HMC3) modulated microglia activation, as shown by CD68 levels. Interestingly, miR142-3p modulation also regulated the production of VEGF-A, the main pro-angiogenic factor. Together, these data strongly support the unprecedented importance of miR-142-3p-dependent vascular-inflammation axis during CNV progression, through microglia activation.
Disciplines :
Ophthalmology
Author, co-author :
Roblain, Quentin ;  Université de Liège - ULiège > GIGA
Louis, Thomas ;  Université de Liège - ULiège > GIGA Cancer - Tumours and development biology
Yip, Cassandre ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, humaines et path.
Baudin, Louis ;  Université de Liège - ULiège > GIGA Cancer - Tumours and development biology
Struman, Ingrid  ;  Université de Liège - ULiège > GIGA Cancer - Molecular Angiogenesis Laboratory
Caolo, Vincenza
LAMBERT, Vincent  ;  Centre Hospitalier Universitaire de Liège - CHU > Autres Services Médicaux > Service d'ophtalmologie
Lecomte, Julie  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Noël, Agnès  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire
Heymans, Stephane 
 These authors have contributed equally to this work.
Language :
English
Title :
Intravitreal injection of anti-miRs against miR-142-3p reduces angiogenesis and microglia activation in a mouse model of laser-induced choroidal neovascularization.
Publication date :
2021
Journal title :
Aging
eISSN :
1945-4589
Publisher :
Impact Journals, Albany, United States - New York
Volume :
13
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 12 June 2021

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