[en] Many stored product insect pests are termed "freeze-intolerant" because they cannot survive ice formation in their extracellular body fluid. In grain silos, the progressive decrease in temperature permits the acclimation of insects and enhances their cold tolerance. The objective of this study was to examine the influence of the concentration of the ice-nucleating-active bacterium Pseudomonas syringae (10, 100 and 1000 ppm), temperature and duration of sub-zero exposure on the cold tolerance of the granary weevil Sitophilus granarius (L.) and the saw-toothed grain beetle Oryzaephilus surinamensis (L.). After an application of 1000 ppm of powdered P. syringae to grain, the mortality of S. granarius and O. surinamensis was increased after 24-h exposure to -4 degrees C. Higher mortality was observed after exposure to colder temperatures and a dose-response relationship was evident. At near-zero (-4 to 0 degrees C) negative temperatures, no dose response was observed, and the mortality in treated grain was the same as that in untreated grain. (C) 1998 Elsevier Science Ltd. All rights reserved.
Disciplines :
Entomology & pest control
Author, co-author :
Mignon, Jacques ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Entomologie fonctionnelle et évolutive
Haubruge, Eric ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Entomologie fonctionnelle et évolutive
Gaspar, Charles ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Entomologie fonctionnelle et évolutive
Language :
English
Title :
Effect of ice-nucleating bacteria (Pseudomonas syringae Van Hall) on insect susceptibility to sub-zero temperatures
Blondeaux A. and Cochet N. (1994a) Ice-nucleating activity of Pseudomonas syringae cultivated on a natural substrate; influence of phosphate. Applied Microbiology and Biotechnology 41, 627-631.
Blondeaux A. and Cochet N. (1994b) High-level expression of the ice-nucleating activity of Pseudomonas syringae in relation to its growth characteristics. Applied Microbiology and Biotechnology 42, 116-120.
Evans D. E. (1983) The influence of relative humidity and thermal acclimation on survival of adult grain beetles in cooled grain. Journal of Stored Products Research 19, 173-180.
Fields, P. G. (1990) The cold-hardiness of Cryptolestes ferrugineus and the use of ice-nucleation-active bacteria as a cold synergist. In Proceedings of the Fifth International Working Conference on Stored-Product Protection, eds F. Fleurat-Lessard and P. Ducom, Vol. II, pp. 1183-1191. Bordeaux, France. 1992.
Fields P. G. (1992) The control of stored-product insects and mites with extreme temperatures. Journal of Stored Products Research 28, 89-118.
Fields P. G. (1993) Reduction of cold tolerance of stored product insects by ice-nucleating-active bacteria. Environmental Entomology 22, 470-476.
Goodnow R. A., Harrison M. D., Morris J. D., Sweeting K. B. and Laduca R. J. (1990a) Fate of ice nucleation-active Pseudomonas syringae strains in alpine soils and waters in synthetic snow samples. Applied and Environmental Microbiology 56, 2223-2227.
Goodnow R. A., Katz G., Haines D. C. and Terril J. (1990b) Two-week inhalation toxicity study of an ice nucleation-active Pseudomonas syringae administered as a respirable aerosol to rats. Toxicological Letters 54, 157-167.
Hunter A. J. and Taylor P. A. (1980) Refrigerated aeration for the preservation of bulk grain. Journal of Stored Products Research 16, 123-131.
Kozloff L. M., Lute M. and Westaway D. (1984) Phosphatidylinositol as a component of the ice nucleating site of Pseudomonas syringae and Erwinia herbicola. Science 226, 845-846.
Lasseran, J. C. and Fleurat-Lessard, F. (1990) Aeration of grain with ambient or artificially cooled grain: a technique to control weevils in temperate climates. In Proceedings of the Fifth International Working Conference on Stored-Product Protection, eds F. Fleurat-Lessard and P. Ducom, Vol. II, pp. 1221-1231. Bordeaux, France, 1992.
Lee R. E., Strong-Gunderson J. M., Lee M. R. and Davidson E. C. (1992) Ice-nucleating-active bacteria decrease the cold-hardiness of stored grain insects. Journal of Economic Entomology 85, 371-374.
Lee R. E., Lee M. R. and Strong-Gunderson J. M. (1993) Insect cold-hardiness and ice nucleating active microorganisms including their potential use for biological control. Journal of Insect Physiology 39, 1-12.
Lindow S. E., Arny D. C. and Upper C. D. (1978) Distribution of ice-nucleating-active bacteria on plants in nature. Applied and Environmental Microbiology 36, 831-836.
Lindow S. E., Arny D. C. and Upper C. D. (1982) Bacterial ice nucleation: a factor in frost injury to plants. Plant Physiology 70, 1084-1089.
MacGlobe (1991) Version 1.0, PC Globe Inc., 4700 South McClintock, Tempe, Arizona 85282.
Maki L. R., Galyan E. L., Chang-Chien M. and Caldwell D. R. (1974) Ice nucleation induced by Pseudomonas syringae. Applied Microbiology 28, 456-459.
Steigerwald K. A., Lee M. R., Lee R. E. and Marshall J. C. (1995) Effect of biological ice nucleators on insect supercooling capacity varies with anatomic site of application. Journal of Insect Physiology 41, 603-608.
Strong-Gunderson J. M., Lee R. E., Lee M. R. and Riga T. J. (1990) Ingestion of ice-nucleating active bacteria increases the supercooling point of the lady beetle Hippodamia convergens. Journal of Insect Physiology 36, 153-157.
Strong-Gunderson J. M., Lee R. E. and Lee M. R. (1992) Topical application of ice-nucleating-active bacteria decreases insect cold tolerance. Applied and Environmental Microbiology 58, 2711-2716.
Wilkinson, L., Hill, M. and Vang, E (1992) SYSTAT: Statistics, Version 5.2 Edition. SYSTAT, Evanston, IL.
Wolber P. K., Deininger C. A., Southworth M. W., Vandekerckhove J., Van Montagu M. and Warren G. J. (1986) Identification and purification of a bacterial ice-nucleation protein. Proceedings of the National Academy of Sciences of the USA 83, 7256-7260.