[en] Using the CRISP/Cas9 technology, we targeted the zebrafish col10a1a gene to insert the Gfp coding sequence into its genome, generating a transgenic zebrafish line expressing a secreted Gfp fusion protein in the developing skeleton that reveals the bone extracellular matrix.
Research center :
GIGA-I3 - Giga-Infection, Immunity and Inflammation - ULiège
Disciplines :
Genetics & genetic processes
Author, co-author :
Muller, Marc ; Université de Liège - ULiège > Département des sciences de la vie > GIGA I3 - Laboratory for Organogenesis and Regeneration
Raman, Ratish ; Université de Liège - ULiège > GIGA I3 - Laboratory for Organogenesis and Regeneration
Guerrero Limon, Gustavo ; Université de Liège - ULiège > GIGA I3 - Laboratory for Organogenesis and Regeneration
Renn, Joerg; Université de Liège - ULiège > GIGA I3 > Laboratory for Organogenesis and Regeneration > Post-Doc
Language :
English
Title :
A reporter zebrafish line for live fluorescent visualisation of bone extracellular matrix
Publication date :
23 September 2020
Event name :
The International CRISPR and Gene Editing Symposium
Event organizer :
rrogers@liebertpub.com;
Event place :
New York, United States
Event date :
23-09-2020
Audience :
International
European Projects :
H2020 - 766347 - BioMedaqu - Aquaculture meets Biomedicine: Innovation in Skeletal Health research.
Name of the research project :
BIOMEDAQU
Funders :
UE - Union Européenne [BE] CE - Commission Européenne [BE]
Commentary :
The zebrafish is increasingly used as a model to study skeletal development and homeostasis. One advantage is the
transparency of the larvae allowing live observation of internal structures. The zebrafish col10a1a gene is expressed in hypertrophic chondrocytes (cartilage) and in osteoblasts (forming bone) and thus is a perfect marker for the developing skeleton.We present a transgenic zebrafish zebrafish line expressing a secreted Gfp fusion
protein in the developing skeleton that reveals the bone extracellular matrix.
