Article (Scientific journals)
Biliary Microbiota and Bile Acid Composition in Cholelithiasis
Petrov, Viacheslav; Fernández-Peralbo, M. A.; Derks, R. et al.
2020In BioMed Research International, 2020
Peer Reviewed verified by ORBi
 

Files


Full Text
1242364.pdf
Publisher postprint (684.01 kB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Acetobacteraceae; Actinomycetales; Article; Bacteroidales; Haemophilus parainfluenzae; Microbacterium; Prevotella intermedia; Sphingomonas; Opisthorchis; Adult; Animals; Bile Acids and Salts; Bile Ducts; Biodiversity; Cholelithiasis; Female; Gallbladder; Humans; Male; Microbiota; Middle Aged; Opisthorchiasis
Abstract :
[en] Background. A functional interplay between BAs and microbial composition in gut is a well-documented phenomenon. In bile, this phenomenon is far less studied, and with this report, we describe the interactions between the BAs and microbiota in this complex biological matrix. Methodology. Thirty-seven gallstone disease patients of which twenty-one with Opisthorchis felineus infection were enrolled in the study. The bile samples were obtained during laparoscopic cholecystectomy for gallstone disease operative treatment. Common bile acid composition was measured by LC-MS/MS. Gallbladder microbiota were previously analyzed with 16S rRNA gene sequencing on Illumina MiSeq platform. The associations between bile acid composition and microbiota were analyzed. Results. Bile acid signature and Opisthorchis felineus infection status exert influence on beta-diversity of bile microbial community. Direct correlations were found between taurocholic acid, taurochenodeoxycholic acid concentrations, and alpha-diversity of bile microbiota. Taurocholic acid and taurochenodeoxycholic acid both show positive associations with the presence of Chitinophagaceae family, Microbacterium and Lutibacterium genera, and Prevotella intermedia. Also, direct associations were identified for taurocholic acid concentration and the presence of Actinomycetales and Bacteroidales orders, Lautropia genus, Jeotgalicoccus psychrophilus, and Haemophilus parainfluenzae as well as for taurochenodeoxycholic acid and Acetobacteraceae family and Sphingomonas genus. There were no differences in bile acid concentrations between O. felineus-infected and noninfected patients. Conclusions/Significance. Associations between diversity, taxonomic profile of bile microbiota, and bile acid levels were evidenced in patients with cholelithiasis. Increase of taurochenodeoxycholic acid and taurocholic acid concentration correlates with bile microbiota alpha-diversity and appearance of opportunistic pathogens. © 2020 Vyacheslav A. Petrov et al.
Disciplines :
Life sciences: Multidisciplinary, general & others
Author, co-author :
Petrov, Viacheslav ;  Siberian State Medical University (SSMU), 2, Moscow Trakt, Tomsk, 634050, Russian Federation
Fernández-Peralbo, M. A.;  Department of Analytical Chemistry, Annex Marie Curie Building, Campus of Rabanales, University of Córdoba, Córdoba, E-14071, Spain
Derks, R.;  Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, Netherlands
Knyazeva, E. M.;  School of Core Engineering Education, National Research Tomsk Polytechnic University, Tomsk, Russian Federation
Merzlikin, N. V.;  Surgical Diseases Department of Pediatric Faculty, Siberian State Medical University, Tomsk, Russian Federation
Sazonov, A. E.;  Central Research Laboratory, Siberian State Medical University, Tomsk, Russian Federation
Mayboroda, O. A.;  Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, Netherlands, Department of Chemistry, Tomsk State University, Tomsk, Russian Federation
Saltykova, I. V.;  Central Research Laboratory, Siberian State Medical University, Tomsk, Russian Federation
Language :
English
Title :
Biliary Microbiota and Bile Acid Composition in Cholelithiasis
Publication date :
2020
Journal title :
BioMed Research International
ISSN :
2314-6133
eISSN :
2314-6141
Publisher :
Hindawi Limited
Volume :
2020
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 27 May 2021

Statistics


Number of views
48 (2 by ULiège)
Number of downloads
44 (1 by ULiège)

Scopus citations®
 
12
Scopus citations®
without self-citations
12
OpenCitations
 
9

Bibliography


Similar publications



Contact ORBi