Biliary Microbiota and Bile Acid Composition in Cholelithiasis

Acetobacteraceae; Actinomycetales; Article; Bacteroidales; Haemophilus parainfluenzae; Microbacterium; Prevotella intermedia; Sphingomonas; Opisthorchis; Adult; Animals; Bile Acids and Salts; Bile Ducts; Biodiversity; Cholelithiasis; Female; Gallbladder; Humans; Male; Microbiota; Middle Aged; Opisthorchiasis

Abstract :

[en] Background. A functional interplay between BAs and microbial composition in gut is a well-documented phenomenon. In bile, this phenomenon is far less studied, and with this report, we describe the interactions between the BAs and microbiota in this complex biological matrix. Methodology. Thirty-seven gallstone disease patients of which twenty-one with Opisthorchis felineus infection were enrolled in the study. The bile samples were obtained during laparoscopic cholecystectomy for gallstone disease operative treatment. Common bile acid composition was measured by LC-MS/MS. Gallbladder microbiota were previously analyzed with 16S rRNA gene sequencing on Illumina MiSeq platform. The associations between bile acid composition and microbiota were analyzed. Results. Bile acid signature and Opisthorchis felineus infection status exert influence on beta-diversity of bile microbial community. Direct correlations were found between taurocholic acid, taurochenodeoxycholic acid concentrations, and alpha-diversity of bile microbiota. Taurocholic acid and taurochenodeoxycholic acid both show positive associations with the presence of Chitinophagaceae family, Microbacterium and Lutibacterium genera, and Prevotella intermedia. Also, direct associations were identified for taurocholic acid concentration and the presence of Actinomycetales and Bacteroidales orders, Lautropia genus, Jeotgalicoccus psychrophilus, and Haemophilus parainfluenzae as well as for taurochenodeoxycholic acid and Acetobacteraceae family and Sphingomonas genus. There were no differences in bile acid concentrations between O. felineus-infected and noninfected patients. Conclusions/Significance. Associations between diversity, taxonomic profile of bile microbiota, and bile acid levels were evidenced in patients with cholelithiasis. Increase of taurochenodeoxycholic acid and taurocholic acid concentration correlates with bile microbiota alpha-diversity and appearance of opportunistic pathogens. © 2020 Vyacheslav A. Petrov et al.

Disciplines :

Life sciences: Multidisciplinary, general & others

Author, co-author :

Petrov, Viacheslav ; Siberian State Medical University (SSMU), 2, Moscow Trakt, Tomsk, 634050, Russian Federation

Fernández-Peralbo, M. A.; Department of Analytical Chemistry, Annex Marie Curie Building, Campus of Rabanales, University of Córdoba, Córdoba, E-14071, Spain

Derks, R.; Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, Netherlands

Knyazeva, E. M.; School of Core Engineering Education, National Research Tomsk Polytechnic University, Tomsk, Russian Federation

Merzlikin, N. V.; Surgical Diseases Department of Pediatric Faculty, Siberian State Medical University, Tomsk, Russian Federation

Sazonov, A. E.; Central Research Laboratory, Siberian State Medical University, Tomsk, Russian Federation

Mayboroda, O. A.; Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, Netherlands, Department of Chemistry, Tomsk State University, Tomsk, Russian Federation

Saltykova, I. V.; Central Research Laboratory, Siberian State Medical University, Tomsk, Russian Federation

Language :

English

Title :

Biliary Microbiota and Bile Acid Composition in Cholelithiasis

Publication date :

2020

Journal title :

BioMed Research International

ISSN :

2314-6133

eISSN :

2314-6141

Publisher :

Hindawi Limited

Volume :

2020

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 27 May 2021

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