The impact of Opisthorchis felineus infection and praziquantel treatment on the intestinal microbiota in children

Children; Gut microbiota; Opisthorchis felineus; Parasite; RNA 16S; RNA; Anaerosporobacter; Article; Bacteroides; Barnesiella; Blautia; Escherichia; Eubacterium eligens; Eubacterium hallii; Faecalitalea; Lachnospira; Megasphaera; Oscillibacter; RNA gene; Roseburia; Ruminiclostridium; Shigella; Bacteria (microorganisms); Enterobacteriaceae; Faecalibacterium; Lachnospiraceae; Microbiota; Vermes

Abstract :

[en] The presence of some species of helminths is associated with changes in host microbiota composition and diversity, which varies widely depending on the infecting helminth species and other factors. We conducted a prospective case-control study to evaluate the gut microbiota in children with Opisthorchis felineus infection (n=50) before and after anthelmintic treatment and in uninfected children (n=49) in the endemic region. A total of 99 children and adolescents aged between 7 and 18 years were enrolled to the study. Helminth infection was assessed before and at 3 months after treatment with praziquantel. A complex examination for each participant was performed in the study, including an assessment of the clinical symptoms and an intestinal microbiota survey by 16S rRNA gene sequencing of stool samples. There was no change in alpha diversity between O. felineus-infected and control groups. We found significant changes in the abundances of bacterial taxa at different taxonomic levels between the infected and uninfected individuals. Enterobacteriaceae family was more abundant in infected participants compared to uninfected children. On the genus level, O. felineus-infected participants’ microbiota showed higher levels of Lachnospira, Escherichia-Shigella, Bacteroides, Eubacterium eligens group, Ruminiclostridium 6, Barnesiella, Oscillibacter, Faecalitalea and Anaerosporobacter and reduction of Blautia, Lachnospiraceae FCS020 and Eubacterium hallii group in comparison with the uninfected individuals. Following praziquantel therapy, there were significant differences in abundances of some microorganisms, including an increase of Faecalibacterium and decrease of Megasphaera, Roseburia. Enterobacteriaceae and Escherichia abundances were decreased up to the control group values. Our results highlight the importance of the host-parasite-microbiota interactions for the community health in the endemic regions. © 2021

Disciplines :

Immunology & infectious disease

Author, co-author :

Sokolova, T. S.; Federal State Budget Educational Institution of Higher Education, Siberian State Medical University, Ministry of Healthcare of Russian Federation, Department of Faculty Pediatrics, Moscowsky tract, 2, Tomsk, 634050, Russian Federation

Petrov, Viacheslav ; Siberian State Medical University (SSMU), 2, Moscow Trakt, Tomsk, 634050, Russian Federation ; RECETOX, Masaryk University, Brno, Czech Republic

Saltykova, I. V.; Federal State Budget Educational Institution of Higher Education, Siberian State Medical University, Ministry of Healthcare of Russian Federation, Central Research Laboratory, Tomsk, Russian Federation

Dorofeeva, Y. B.; Federal State Budget Educational Institution of Higher Education, Siberian State Medical University, Ministry of Healthcare of Russian Federation, Biology and genetics department, Tomsk, Russian Federation

Tyakht, A. V.; Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Institute of Gene Biology of Russian Academy of Science, Moscow, Russian Federation, Atlas Biomed Group - Knomics LLC, London, United Kingdom

Ogorodova, L. M.; Federal State Budget Educational Institution of Higher Education, Siberian State Medical University, Ministry of Healthcare of Russian Federation, Department of Faculty Pediatrics, Moscowsky tract, 2, Tomsk, 634050, Russian Federation

Fedorova, O. S.; Federal State Budget Educational Institution of Higher Education, Siberian State Medical University, Ministry of Healthcare of Russian Federation, Department of Faculty Pediatrics, Moscowsky tract, 2, Tomsk, 634050, Russian Federation

Title :

The impact of Opisthorchis felineus infection and praziquantel treatment on the intestinal microbiota in children

Publication date :

2021

Journal title :

Acta Tropica

ISSN :

0001-706X

Publisher :

Elsevier B.V.

Volume :

217

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

Russian Foundation for Basic Research, РФФИ: N19-515-70004

Available on ORBi :

since 27 May 2021

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