Memristive biosensors based on full-size antibodies and antibody fragments

Antibody fragments; Cancer diagnosis; Memristive biosensors; Silicon nanowires; Antibodies; Antigens; Biosensors; Diseases; Morphology; Nanowires; Scanning electron microscopy; Surface morphology; Urology; Antibody fragment; Label-free detection; Prostate specific antigen; Silicon nanowire arrays; Single-chain antibodies; Chemical detection

Abstract :

[en] For the first time memristive biosensors developed by the incorporation of scAbs (single-chain antibody fragments) enabling label-free detection of free Prostate Specific Antigen (fPSA) for early-stage detection of prostate cancer (PC) are reported. In addition, the first ever-published report of a direct relationship between the size of the bio-probes used to transfer specificity to the sensor and the voltage gap is here included. The proposed bio-detection is achieved by means of a voltage gap acquired in the electrical response of memristive sensors obtained by the bio-modification of silicon nanowire arrays. A comparison between memristive biosensors based on full-size antibodies and antibody fragments is also demonstrated by analysis of the surface morphology via Scanning Electron Microscopy. This new kind of biosensors provide excellent analytical sensitivity with a limit-of-detection down to 0.144 pg/mL on PSA. © 2019 Elsevier B.V.

Disciplines :

Chemistry

Author, co-author :

Tzouvadaki, I.; Integrated System Laboratory, École Polytechnique Fédérale de Lausanne, Switzerland

Zapatero-Rodríguez, J.; School of Biotechnology, Dublin City University, Dublin 9, Ireland

Naus, Sébastien ; Université de Liège - ULiège

de Micheli, G.; Integrated System Laboratory, École Polytechnique Fédérale de Lausanne, Switzerland

O'Kennedy, R.; School of Biotechnology, Dublin City University, Dublin 9, Ireland, Research, Development and Innovation, Qatar Foundation, Qatar

Carrara, S.; Integrated System Laboratory, École Polytechnique Fédérale de Lausanne, Switzerland

Language :

English

Title :

Memristive biosensors based on full-size antibodies and antibody fragments

Publication date :

2019

Journal title :

Sensors and Actuators. B, Chemical

ISSN :

0925-4005

eISSN :

1873-3077

Publisher :

Elsevier Sequoia, Lausanne, Switzerland

Volume :

286

Pages :

346-352

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 21 May 2021

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