Memristive biosensors based on full-size antibodies and antibody fragments

Antibody fragments; Cancer diagnosis; Memristive biosensors; Silicon nanowires; Antibodies; Antigens; Biosensors; Diseases; Morphology; Nanowires; Scanning electron microscopy; Surface morphology; Urology; Antibody fragment; Label-free detection; Prostate specific antigen; Silicon nanowire arrays; Single-chain antibodies; Chemical detection

Abstract :

[en] For the first time memristive biosensors developed by the incorporation of scAbs (single-chain antibody fragments) enabling label-free detection of free Prostate Specific Antigen (fPSA) for early-stage detection of prostate cancer (PC) are reported. In addition, the first ever-published report of a direct relationship between the size of the bio-probes used to transfer specificity to the sensor and the voltage gap is here included. The proposed bio-detection is achieved by means of a voltage gap acquired in the electrical response of memristive sensors obtained by the bio-modification of silicon nanowire arrays. A comparison between memristive biosensors based on full-size antibodies and antibody fragments is also demonstrated by analysis of the surface morphology via Scanning Electron Microscopy. This new kind of biosensors provide excellent analytical sensitivity with a limit-of-detection down to 0.144 pg/mL on PSA. © 2019 Elsevier B.V.

Disciplines :

Chemistry

Author, co-author :

Tzouvadaki, I.; Integrated System Laboratory, École Polytechnique Fédérale de Lausanne, Switzerland

Zapatero-Rodríguez, J.; School of Biotechnology, Dublin City University, Dublin 9, Ireland

Naus, Sébastien ; Université de Liège - ULiège

de Micheli, G.; Integrated System Laboratory, École Polytechnique Fédérale de Lausanne, Switzerland

O'Kennedy, R.; School of Biotechnology, Dublin City University, Dublin 9, Ireland, Research, Development and Innovation, Qatar Foundation, Qatar

Carrara, S.; Integrated System Laboratory, École Polytechnique Fédérale de Lausanne, Switzerland

Language :

English

Title :

Memristive biosensors based on full-size antibodies and antibody fragments

Publication date :

2019

Journal title :

Sensors and Actuators. B, Chemical

ISSN :

0925-4005

eISSN :

1873-3077

Publisher :

Elsevier Sequoia, Lausanne, Switzerland

Volume :

286

Pages :

346-352

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 21 May 2021

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Bibliography

Doucey, M.A., Carrara, S., Nanowire sensors in cancer. Trends Biotechnol., 2018, 10.1016/j.tibtech.2018.07.014.
Zheng, G., Patolsky, F., Cuil, Y., Wang, W., Lieber, C., Multiplexed electrical detection of cancer markers with nanowire sensor arrays. Nat. Biotechnol. 23:3 (2005), 1294–1301.
Sharma, S., Byrne, H., O'Kennedy, R.J., Antibodies and antibody-derived analytical biosensors. Essays Biochem. 60:1 (2016), 9–18.
Belicky, S., Damborsky, P., Zapatero-Rodríguez, J., O'Kennedy, R., Tkac, J., Full-length antibodies versus single chain antibody fragments for a selective impedimetric lectin-based glycoprofiling of prostate specific antigen. Electrochim. Acta 246 (2017), 399–405.
Chua, L., Memristor-the missing circuit element. IEEE Trans. Circuit Theory, 18(5), 1971.
Sacchetto, D., Gaillardon, P.E., Zervas, M., Carrara, S., De Micheli, G., Leblebici, Y., Applications of multi-terminal memristive devices: a review. IEEE Circuits Syst. Mag. 13:2 (2013), 23–41.
Carrara, S., Sacchetto, D., Doucey, M.A., Baj-Rossi, C., De Micheli, G., Leblebici, Y., Memristive-biosensors: a new detection method by using nanofabricated memristors. Sens. Actuators, B 171 (2012), 449–457.
Zapatero-Rodríguez, J., Liébana, S., Gilgunn, S., Sharma, S., O'Kennedy, R., Detection of free prostate specific antigen using a novel single-chain antibody (scAb)-based magneto-immunosensor. BioNanoScience 8:2 (2018), 680–689.
Hayhurst, A., Happe, S., Mabry, R., Koch, Z., Iverson, B.L., Georgiou, G., Isolation and expression of recombinant antibody fragments to the biological warfare pathogen Brucella melitensis. J. Immunol. Methods 276:1 (2003), 185–196.
Viguier, C., Crean, C., O'Kennedy, R., Trends and perspectives in immunosensors. Antibodies Applications and New Developments, Bentham Books, 2012, 184–208.
Armbruster, D.A., Pry, T., Limit of blank, limit of detection and limit of quantitation. Clin. Biochem. Rev., 29(Suppl. 1), 2008, S49.
Chuah, K., Lai, L.M., Goon, I.Y., Parker, S.G., Amal, R., Gooding, J.J., Ultrasensitive electrochemical detection of prostate-specific antigen (PSA) using gold-coated magnetic nanoparticles as ‘dispersible electrodes’. Chem. Commun. 48:29 (2012), 3503–3505.
Kavosi, B., Salimi, A., Hallaj, R., Moradi, F., Ultrasensitive electrochemical immunosensor for PSA biomarker detection in prostate cancer cells using gold nanoparticles/PAMAM dendrimer loaded with enzyme linked aptamer as integrated triple signal amplification strategy. Biosens. Bioelectron. 74 (2015), 915–923.
Escamilla-Gómez, V., Hernández-Santos, D., González-García, M.B., Pingarrón-Carrazón, J.M., Costa-García, A., Simultaneous detection of free and total prostate specific antigen on a screen-printed electrochemical dual sensor. Biosens. Bioelectron. 24:8 (2009), 2678–2683.
Jang, H.D., Kim, S.K., Chang, H., Choi, J.W., 3D label-free prostate specific antigen (PSA) immunosensor based on graphene–gold composites. Biosens. Bioelectron. 63 (2015), 546–551.
Sarkar, P., Ghosh, D., Bhattacharyay, D., Setford, S.E., Turner, A.E.E., Electrochemical immunoassay for Free prostate specific antigen (f‐PSA) using magnetic beads. Electroanalysis 20:13 (2008), 1414–1420.
Spain, E., Gilgunn, S., Sharma, S., Adamson, K., Carthy, E., O'Kennedy, R., Forster, R.J., Detection of prostate specific antigen based on electrocatalytic platinum nanoparticles conjugated to a recombinant scFv antibody. Biosens. Bioelectron. 77 (2016), 759–766.
Salimi, A., Kavosi, B., Fathi, F., Hallaj, R., Highly sensitive immunosensing of prostate-specific antigen based on ionic liquid–carbon nanotubes modified electrode: application as cancer biomarker for prostatebiopsies. Biosens. Bioelectron. 42 (2013), 439–446.
Zhao, J., Guo, Z., Feng, D., Guo, J., Wang, J., Zhang, Y., Simultaneous electrochemical immunosensing of alpha-fetoprotein and prostate specific antigen using a glassy carbon electrode modified with gold nanoparticle-coated silica nanospheres and decorated with azure a or ferrocenecarboxylic acid. Microchim. Acta, 182, 2015.
Han, J., Zhuo, Y., Chai, Y., Yuan, R., Zhang, W., Zhu, Q., Simultaneous electrochemical detection of multiple tumor markers based on dual catalysis amplification of multi-functionalized onion-like mesoporous graphene sheets. Anal. Chim. Acta 746 (2012), 70–76.
Yang, M., Javadi, A., Gong, S., Sensitive electrochemical immunosensor for the detection of cancer biomarker using quantum dot functionalized graphene sheets as labels. Sens. Actuator B-Chem. 155:1 (2011), 357–360.
Rafique, S., Bin, W., Bhatti, A.S., Electrochemical immunosensor for prostate-specific antigens using a label-free second antibody based on silica nanoparticles and polymer brush. Bioelectrochemistry 101 (2015), 75–83.
Chen, X., Zhou, G., Song, P., Wang, J., Gao, J., Lu, J., Fan, C., Zuo, X., Ultrasensitive electrochemical detection of prostate-specific antigen by using antibodies anchored on a DNA nanostructural scaffold. Anal. Chem. 86:15 (2014), 7337–7342.
Chornokur, G., Arya, S.K., Phelan, C., Tanner, R., Bhansali, S., Impedance-based miniaturized biosensor for ultrasensitive and fast prostate-specific antigen detection. J. Sens. 983752 (2011), 1–7.
Fan, D., Li, N., Ma, H., Li, Y., Hu, L., Du, B., Wei, Q., Electrochemical immunosensor for detection of prostate specific antigen based on an acid cleavable linker into MSN-based controlled release system. Biosens. Bioelectron. 85 (2016), 580–586.
Tzouvadaki, I., Jolly, P., Lu, X., Ingebrandt, S., de Micheli, G., Estrela, P., Carrara, S., Label-free ultrasensitive memristive aptasensor. Nano Lett. 16 (2016), 4472–4476.