Article (Scientific journals)
COVID-19-associated nephropathy includes tubular necrosis and capillarycongestion, with evidence of SARS-CoV-2 in the nephron
BOUQUEGNEAU, Antoine; ERPICUM, Pauline; GROSCH, Stéphanie et al.
2021In Kidney International
Peer Reviewed verified by ORBi
 

Files


Full Text
KID.0006992020.full.pdf
Publisher postprint (10.36 MB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
SARS-CoV-2; acute kidney injury; tubular necrosis; capillary congestion; renal parenchyma
Abstract :
[en] Background: Kidney damage has been reported in patients with COVID-19. Despite numerous reports about COVID-19-associated nephropathy, the factual presence of the SARS-CoV-2 in the renal parenchyma remains controversial. Methods: We consecutively performed 16 immediate (≤3h) post-mortem renal biopsies in patients diagnosed with COVID-19. Kidney samples from 5 patients who died from sepsis not related to COVID-19 were used as controls. Samples were methodically evaluated by 3 pathologists. Virus detection in the renal parenchyma was performed in all samples by bulk RNA RT-PCR (E and N1/N2 genes), immunostaining (nCoV2019 N-Protein), fluorescent in situ hybridization (nCoV2019-S) and electron microscopy. Results: The mean age of our COVID-19 cohort was 68.2±12.8 years, most of whom were males (68.7%). Proteinuria was observed in 53.3% of cases, while acute kidney injury occurred in 60% of cases. Acute tubular necrosis of variable severity was found in all cases, with no tubular or interstitial inflammation. There was no difference in acute tubular necrosis severity between the patients with COVID-19 versus controls. Congestion in glomerular and peri tubular capillaries was respectively observed in 56.3 and 87.5% of patients with COVID-19 compared to 20% of controls, with no evidence of thrombi. The nCoV2019 N-Protein was detected in proximal tubules and also at the basolateral pole of scattered cells of the distal tubules in 9/16 cases. In situ hybridization confirmed these findings in 6/16 cases. RT-PCR of kidney total RNA detected SARS-CoV-2 E and N1/N2 genes in one case. Electron microscopy did not show typical viral inclusions. Conclusions: Our immediate post-mortem kidney samples from patients with COVID-19 highlight a congestive pattern of acute kidney injury, with no significant glomerular or interstitial inflammation. Immunostaining and in situ hybridization suggest that SARS-CoV-2 is present in various segments of the nephron.
Disciplines :
Laboratory medicine & medical technology
Urology & nephrology
General & internal medicine
Author, co-author :
BOUQUEGNEAU, Antoine  ;  Centre Hospitalier Universitaire de Liège - CHU > Département de médecine interne > Service de néphrologie
ERPICUM, Pauline ;  Centre Hospitalier Universitaire de Liège - CHU > Département de médecine interne > Service de néphrologie
GROSCH, Stéphanie ;  Centre Hospitalier Universitaire de Liège - CHU > Département de médecine interne > Service de néphrologie
HABRAN, Lionel ;  Centre Hospitalier Universitaire de Liège - CHU > Unilab > Laboratoire biologie moléculaire
HOUGRAND, Olivier  ;  Centre Hospitalier Universitaire de Liège - CHU > Unilab > Laboratoire microscopie électronique
HUART, Justine  ;  Centre Hospitalier Universitaire de Liège - CHU > Département de médecine interne > Service de néphrologie
KRZESINSKI, Jean-Marie ;  Centre Hospitalier Universitaire de Liège - CHU > Département de médecine interne > Service de néphrologie
Misset, Benoît ;  Université de Liège - ULiège > Département des sciences cliniques > Soins intensifs
Hayette, Marie-Pierre ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Bact., mycologie, parasitologie, virologie, microbio.
DELVENNE, Philippe ;  Centre Hospitalier Universitaire de Liège - CHU > Unilab > Service d'anatomie et cytologie pathologiques
BOVY, Christophe ;  Centre Hospitalier Universitaire de Liège - CHU > Département de médecine interne > Service de néphrologie
Kylies, dominique;  University of Hamburg > Department of Internal Medicine > Nephrotology/Rheumatology with Section Endocrinology
huber, Tobias B.;  Albert-Ludwigs-Universität Freiburg > Medicine/Nephrology/Molecular Medicine > Pr. Dr. Internal Senior Fellow
Puelles, Victor G.;  University Medical Center Hamburg > Department of Internal Medicine > Nephrology
DELANAYE, Pierre  ;  Centre Hospitalier Universitaire de Liège - CHU > Département de médecine interne > Service de néphrologie
Jouret, François  ;  Université de Liège - ULiège > GIGA Cardio. Sc. - Lab. of Translational Res. in Nephrology
More authors (6 more) Less
Language :
English
Title :
COVID-19-associated nephropathy includes tubular necrosis and capillarycongestion, with evidence of SARS-CoV-2 in the nephron
Publication date :
12 February 2021
Journal title :
Kidney International
ISSN :
0085-2538
eISSN :
1523-1755
Publisher :
Elsevier, United States - New York
Special issue title :
Kidney 360
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 30 April 2021

Statistics


Number of views
192 (33 by ULiège)
Number of downloads
6 (6 by ULiège)

Scopus citations®
 
17
Scopus citations®
without self-citations
15
OpenCitations
 
9

Bibliography


Similar publications



Contact ORBi