Article (Scientific journals)
Angiostrongylosis in dogs with negative fecal and in-clinic rapid serological tests: 7 Cases (2013-2017)
Canonne, A. M.; Billen, Frédéric; Losson, Bertrand et al.
2018In Journal of Veterinary Internal Medicine, 32 (3), p. 951-955
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Keywords :
AngioDetect
Abstract :
[en] Background: Angiostrongylosis is considered as emerging disease in dogs in Belgium. Detection of first-stage larvae in feces using the Baermann method has an imperfect sensitivity. Objectives: Investigation of efficacy of noninvasive blood and fecal diagnostic tests in comparison with PCR on bronchoalveolar lavage (BAL) material in a small series of coughing or dyspnoeic dogs naturally infected with Angiostrongylus vasorum. Animals: Seven dogs with angiostrongylosis. Methods: Retrospective study. Dogs with cough, exercise intolerance and dyspnea of 2- to 8-week duration. Diagnostic methods used included Baermann analysis, AngioDetect rapid assay, ELISAs for detection of circulating antigen and specific antibodies and qPCR on BAL material. Results: Baermann analysis, AngioDetect rapid assay, antigen ELISA, antibody ELISA, and qPCR on BAL material were positive in 3/7, 2/7, 3/6, 6/6, and 7/7 dogs, respectively. ELISA for antibodies or qPCR on BAL material were essential for definitive diagnosis in 3 dogs. Relative sensitivities of AngioDetect rapid assay, Baermann analysis, and ELISA for antigen detection were lower than 50% compared with ELISA for antibodies or qPCR on BAL material. Conclusion and Clinical Importance: In this small clinical series, Baermann analysis and AngioDetect rapid assay failed to confirm the diagnosis in some dogs. Therefore, ELISA for antibody detection and qPCR on BAL material should strongly be considered in clinically suspected dogs when antigen detection methods (AngioDetect or ELISA) and Baermann analysis are negative. Copyright © 2018 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Canonne, A. M.;  Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Liège, Belgium
Billen, Frédéric  ;  Université de Liège - ULiège > Dép. clinique des animaux de compagnie et des équidés (DCA) > Pathologie médicale des petits animaux
Losson, Bertrand ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Parasitologie et pathologie des maladies parasitaires
Peters, I.;  TDDS Laboratories, University of Exeter, Exeter, Devon, London, United Kingdom
Schnyder, M.;  Institute of Parasitology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland
Clercx, Cécile  ;  Université de Liège - ULiège > Dép. clinique des animaux de compagnie et des équidés (DCA) > Pathologie médicale des petits animaux
Language :
English
Title :
Angiostrongylosis in dogs with negative fecal and in-clinic rapid serological tests: 7 Cases (2013-2017)
Publication date :
May 2018
Journal title :
Journal of Veterinary Internal Medicine
ISSN :
0891-6640
eISSN :
1939-1676
Publisher :
Blackwell Publishing Inc.
Volume :
32
Issue :
3
Pages :
951-955
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 28 April 2021

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