[en] In the present study, chromatic coordinates, phenolic acids, flavonoids and antioxidant capacity assessed by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonate (ABTS) and lipid peroxidation inhibition capacity (LPIC) essays and their relative IC50 were investigated in 25 fig cultivars growing in Morocco. The aims of this study were to determine (i) the variation in these compounds among light and dark-colored cultivars, (ii) their partitioning between fruit peel and pulp and (iii) to display network connections among these variables. Twelve phenolic compounds (PCs) were isolated in peel extract versus eight in pulp samples. Anthocyanins, mainly cyanidin-3,5-diglucoside and cyanidin-3-O-rutinoside, were the predominant compounds in peels, where the mean concentrations were 75.90 ± 18.76 and 77.97 ± 18.95 µg/g dw, respectively. On the other hand, (−)-epicatechin and cyanidin-3-O-rutinoside were the major compounds in the pulp extracts, where the mean values were 5.23 ± 4.03 and 9.01 ± 5.67 µg/g dw, respectively. A two-dimensional hierarchically clustered heatmap was applied to the dataset to explore correlations in the dataset and similarities between cultivars, without dimensionality reduction. Results showed that anthocyanins, particularly pelargonidin-3-O-rutinoside, cyanidin-3,5-diglucoside and cyanidin-3-O-rutinoside, were the main contributors to the peels’ free radical scavenging capacity. This capacity was particularly higher in the peel of dark-colored figs compared to the fruit pulp. The local cultivar “INRA 1301” showed the most promising phenolic profile due to its very high levels of almost all detected PCs, especially (−)-epicatechin, quercetin-3-O-rutinoside, quercetin-3-O-glucoside, cyanidine-3,5-diglucoside, cyanidine-3-O-rutinoside and pelargonidin-3-O-rutinoside (54.66, 141.08, 35.48, 494.08, 478.66, 12.56 µg/g dw, respectively). Having the darkest figs in the collection (L* = 25.72, c* = 22.09 and h° = 20.99), this cultivar has also combined promising IC50 values, which were of 19.85, 40.58 and 124.78 µg/mL for DPPH, ABTS and LPIC essays, respectively
Disciplines :
Agriculture & agronomy Chemistry Food science
Author, co-author :
Hssaini, Lahcen
Hernandez, Fransisca
Viueluda-Martos, Manuel
Charafi, Jamal
Razouk, Rachid
Houmanat, Karim
OUAABOU, Rachida
Ennahli, Said
Elothmani, Driss
Hmid, Ilham
Fauconnier, Marie-Laure ; Université de Liège - ULiège > Département GxABT > Chimie des agro-biosystèmes
Hanine, Hafida
Language :
English
Title :
Survey of phenolic acids, flavonoids and antioxidant potency between figs peels and pulps: Chemical and Chemometric Approach
Publication date :
28 April 2021
Journal title :
Molecules
eISSN :
1420-3049
Publisher :
Multidisciplinary Digital Publishing Institute (MDPI), Switzerland
Česonienė, L.; Jasutienė, I.; Šarkinas, A. Phenolics and anthocyanins in berries of European cranberry and their antimicrobial activity. Medicina 2009, 45, 992. [CrossRef]
Tsimogiannis, D.; Oreopoulou, V. Classification of Phenolic Compounds in Plants. In Polyphenols in Plants; Academic Press: Cambridge, MA, USA, 2019; pp. 263–284.
Rispail, N.; Morris, P.; Webb, K.J. Phenolic compounds: Extraction and analysis. In Lotus Japonicus Handbook; Springer: Dordrecht, The Netherlands, 2005; pp. 349–354.
Laura, A.; Moreno-Escamilla, J.O.; Rodrigo-García, J.; Alvarez-Parrilla, E. Phenolic compounds. In Postharvest Physiology and Biochemistry of Fruits and Vegetables; Woodhead Publishing: Cambridge, UK, 2019; pp. 253–271.
Winkel-Shirley, B. Biosynthesis of flavonoids and effects of stress. Curr. Plant Biol. 2002, 5, 218–223. [CrossRef]
Gould, K.S. Muriel wheldale onslow and the rediscovery of anthocyanin function in plants. Recent Adv. Polyphen. Res. 2010, 2, 206–225.
Khoddami, A.; Wilkes, M.A.; Roberts, T.H. Techniques for analysis of plant phenolic compounds. Molecules 2013, 18, 2328–2375. [CrossRef]
Shimazu, T.; Inoue, M.; Sasazuki, S.; Iwasaki, M.; Sawada, N.; Yamaji, T. Isoflavone intake and risk of lung cancer: A prospective cohort study in Japan. Japan Public Health Center–based Prospective Study Group. Am. J. Clin. Nutr. 2010, 91, 722–728. [CrossRef] [PubMed]
Redman, L.M.; Smith, S.R.; Burton, J.H.; Martin, C.K.; Il’yasova, D.; Ravussin, E. Metabolic slowing and reduced oxidative damage with sustained caloric restriction support the rate of living and oxidative damage theories of aging. Cell Metab. 2018, 27, 805–815. [CrossRef]
Lima, C.F.; Fernandes-Ferreira, M.; Pereira-Wilson, C. Phenolic compounds protect HepG2 cells from oxidative damage: Relevance of glutathione levels. Life Sci. 2006, 79, 2056–2068. [CrossRef]
Afonso, M.S.; De O Silva, A.M.; Carvalho, E.B.; Rivelli, D.P.; Barros, S.B.; Rogero, M.M.; Mancini-Filho, J. Phenolic compounds from Rosemary (Rosmarinus officinalis L.) attenuate oxidative stress and reduce blood cholesterol concentrations in diet-induced hypercholesterolemic rats. Nutr. Metab. 2013, 10, 19. [CrossRef]
Le Lay, S.; Simard, G.; Martinez, M.C.; Andriantsitohaina, R. Oxidative stress and metabolic pathologies: From an adipocentric point of view. Oxidative Med. Cell Longev. 2014, 908539. [CrossRef] [PubMed]
Zhang, H.; Tsao, R. Dietary polyphenols, oxidative stress and antioxidant and anti-inflammatory effects. Curr. Opin. Food Sci. 2016, 8, 33–42. [CrossRef]
Tawfeek, N.; Sobeh, M.; Hamdan, D.I.; Farrag, N.; Roxo, M.; El-Shazly, A.M.; Wink, M. Phenolic compounds from Populus alba L. and Salix subserrata Willd. (Salicaceae) counteract oxidative stress in Caenorhabditis elegans. Molecules 2019, 24, 1999. [CrossRef] [PubMed]
Bautista, I.; Boscaiu, M.; Lidón, A.; Llinares, J.V.; Lull, C.; Donat, M.P.; Vicente, O. Environmentally induced changes in antioxidant phenolic compounds levels in wild plants. Acta Physiol. Plant 2016, 38, 9. [CrossRef]
Hssaini, L.; Charafi, J.; Hanine, H.; Ennahli, S.; Mekaoui, A.; Mamouni, A.; Razouk, R. Comparative analysis and physio-biochemical screening of an ex-situ fig (Ficus carica L.) collection. Hortic. Environ. Biotechnol. 2019, 60, 671–683. [CrossRef]
USDA. U.S. Department of Agriculture, Agricultural Research Service. Database for the Flavonoid Content of Selected Foods, Release 3.0; Nutrient Data Laboratory: Beltsville, MD, USA, 2011.
Hssaini, L.; Hanine, H.; Razouk, R.; Ennahli, S.; Mekaoui, A.; Guirrou, I.; Charafi, J. Diversity Screening of Fig (Ficus Carica, L.) Germplasm through Integration of Morpho-agronomic and Biochemical Traits. Int. J. Fruit Sci. 2019, 20, 1–20. [CrossRef]
Vinson, J.A.; Hao, Y.; Su, X.; Zubik, L. Phenol antioxidant quantity and quality in foods: Vegetables. J. Agric. Food Chem. 1998, 46, 3630–3634. [CrossRef]
Hssaini, L.; Charafi, J.; Razouk, R.; Hernández, F.; Fauconnier, M.L.; Ennahli, S.; Hanine, H. Assessment of Morphological Traits and Fruit Metabolites in Eleven Fig Varieties (Ficus Carica L.). Int. J. Fruit Sci. 2020, 1–21, 8–28. [CrossRef]
Stinco, C.M.; Rodríguez-Pulido, F.J.; Escudero-Gilete, M.L.; Gordillo, B.; Vicario, I.M.; Meléndez-Martínez, A.J. Lycopene isomers in fresh and processed tomato products: Correlations with instrumental color measurements by digital image analysis and spectroradiometry. Food Res. Int. 2013, 50, 111–120. [CrossRef]
Kuś, P.M.; Congiu, F.; Teper, D.; Sroka, Z.; Jerković, I.; Tuberoso, C.I.G. Antioxidant activity, color characteristics, total phenol content and general HPLC fingerprints of six Polish unifloral honey types. LWT-Food Sci. Technol. 2014, 55, 124–130.
Silva, B.M.; Andrade, P.B.; Ferreres, F.; Domingues, A.L.; Seabra, R.M.; Ferreira, M.A. Phenolic profile of quince fruit (Cydonia oblonga Miller) (pulp and peel). J. Agric. Food Chem. 2002, 50, 4615–4618. [CrossRef]
Fan, X.; Jiao, W.; Wang, X.; Cao, J.; Jiang, W. Polyphenol composition and antioxidant capacity in pulp and peel of apricot fruits of various varieties and maturity stages at harvest. Int. J. Food Sci. Technol. 2018, 53, 327–336. [CrossRef]
Yang, Y.; Yao, G.; Yue, W.; Zhang, S.; Wu, J. Transcriptome profiling reveals differential gene expression in proanthocyanidin biosynthesis associated with red/green skin color mutant of pear (Pyrus communis L.). Front. Plant Sci. 2015, 6, 795. [CrossRef] [PubMed]
Çalişkan, O.; Polat, A.A. Phytochemical and antioxidant properties of selected fig (Ficus carica L.) accessions from the eastern Mediterranean region of Turkey. Sci. Hortic. 2011, 128, 473–478.
Del Caro, A.; Piga, A. Polyphenol composition of peel and pulp of two Italian fresh fig fruits cultivars (Ficus carica L.). Eur. Food Res. Technol. 2008, 226, 715–719. [CrossRef]
Solomon, A.; Golubowicz, S.; Yablowicz, Z.; Grossman, S.; Bergman, M.; Gottlieb, H.E.; Flaishman, M.A. Antioxidant activities and anthocyanin content of fresh fruits of common fig (Ficus carica L.). J. Agric. Food Chem. 2006, 54, 7717–7723. [CrossRef]
Harzallah, A.; Bhouri, A.M.; Amri, Z.; Soltana, H.; Hammami, M. Phytochemical content and antioxidant activity of different fruit parts juices of three figs (Ficus carica L.) varieties grown in Tunisia. Ind. Crop. Prod. 2016, 83, 255–267. [CrossRef]
Palmeira, L.; Pereira, C.; Dias, M.I.; Abreu, R.M.; Corrêa, R.C.; Pires, T.C.; Ferreira, I.C. Nutritional, chemical and bioactive profiles of different parts of a Portuguese common fig (Ficus carica L.) variety. Food Res. Int. 2019, 126, 108572. [CrossRef] [PubMed]
Tomás-Barberán, F.A.; Gil, M.I.; Cremin, P.; Waterhouse, A.L.; Hess-Pierce, B.; Kader, A.A. HPLC−DAD−ESIMS analysis of phenolic compounds in nectarines, peaches, and plums. J. Agric. Food Chem. 2001, 49, 4748–4760. [CrossRef]
Kamiloglu, S.; Capanoglu, E. Polyphenol content in figs (Ficus carica L.): Effect of sun-drying. Int. J. Food Prop. 2015, 18, 521–535. [CrossRef]
Viuda-Martos, M.; Barber, X.; Pérez-Álvarez, J.A.; Fernández-López, J. Assessment of chemical, physico-chemical, techno-functional and antioxidant properties of fig (Ficus carica L.) powder co-products. Ind. Crop. Prod. 2015, 69, 472–479. [CrossRef]
Pande, G.; Akoh, C.C. Organic acids, antioxidant capacity, phenolic content and lipid characterisation of Georgia-grown underutilized fruit crops. Food Chem. 2010, 120, 1067–1075. [CrossRef]
Ammar, S.; Del Mar Contreras, M.; Belguith-Hadrich, O.; Segura-Carretero, A.; Bouaziz, M. Assessment of the distribution of phenolic compounds and contribution to the antioxidant activity in Tunisian fig leaves, fruits, skins and pulps using mass spectrometry-based analysis. Food Funct. 2015, 6, 3663–3677. [CrossRef]
Konak, R.; Kösoğlu, İ.; Yemenıcıoğlu, A. Effects of different drying methods on phenolic content, antioxidant capacity and general characteristics of selected dark colored Turkish fig cultivars. V Int. Symp. Fig. 2015, 1173, 335–340. [CrossRef]
Chiva-Blanch, G.; Visioli, F. Polyphenols and health: Moving beyond antioxidants. J. Berry Res. 2012, 2, 63–71. [CrossRef]
Martínez-Valverde, I.; Periago, M.J.; Provan, G.; Chesson, A. Phenolic compounds, lycopene and antioxidant activity in commer-cial varieties of tomato (Lycopersicum esculentum). J. Sci. Food Agric. 2002, 82, 323–330. [CrossRef]
Rothwell, J.A.; Perez-Jimenez, J.; Neveu, V.; Medina-Remon, A.; M’Hiri, N.; García-Lobato, P.; Scalbert, A. Phenol-Explorer 3.0: A major update of the Phenol-Explorer database to incorporate data on the effects of food processing on polyphenol content. Database 2013, 2013, bat070. [CrossRef] [PubMed]
Clark, N.R.; Ma’ayan, A. Introduction to statistical methods to analyze large data sets: Principal components analysis. Sci. Signal 2011, 4, tr3. [CrossRef] [PubMed]
Xie, L.; Bolling, B.W. Characterisation of stilbenes in California almonds (Prunus dulcis) by UHPLC–MS. Food Chem. 2014, 148, 300–306. [CrossRef] [PubMed]
Singleton, V.L.; Orthofer, R.; Lamuela-Raventós, R.M. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. In Methods in Enzymology; Academic Press: Cambridge, MA, USA, 1999; Volume 299, pp. 152–178.
Barreira, J.C.; Ferreira, I.C.; Oliveira, M.B.P.; Pereira, J.A. Antioxidant activities of the extracts from chestnut flower, leaf, skins and fruit. Food Chem. 2008, 107, 1106–1113. [CrossRef]
Cheng, G.W.; Breen, P.J. Activity of phenylalanine ammonia-lyase (PAL) and concentrations of anthocyanins and phenolics in developing strawberry fruit. J. Am. Soc. Hortic. Sci. 1991, 116, 865–869. [CrossRef]
Porter, L.J.; Hrstich, L.N.; Chan, B.G. The conversion of procyanidins and prodelphinidins to cyanidin and delphinidin. Phyto-chemistry 1985, 25, 223–230. [CrossRef]
Brand-Williams, W.; Cuvelier, M.E.; Berset, C.L.W.T. Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci. Technol. 1995, 28, 25–30. [CrossRef]
Re, R.; Pellegrini, N.; Proteggente, A.; Pannala, A.; Yang, M.; Rice-Evans, C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 1999, 26, 1231–1237. [CrossRef]
Freire, A.L.; Ramos, C.L.; Da Costa Souza, P.N.; Cardoso, M.G.B.; Schwan, R.F. Nondairy beverage produced by controlled fermentation with potential probiotic starter cultures of lactic acid bacteria and yeast. Int. J. Food Microbiol. 2017, 248, 39–46. [CrossRef]
Genskowsky, E.; Puente, L.A.; Pérez-Álvarez, J.A.; Fernández-López, J.; Muñoz, L.A.; Viuda-Martos, M. Determination of polyphenolic profile, antioxidant activity and antibacterial properties of maqui [Aristotelia chilensi s (Molina) Stuntz] a Chilean blackberry. J. Sci. Food Agric. 2016, 96, 4235–4242. [CrossRef] [PubMed]
Haarman, B.C.B.; Riemersma-Van der Lek, R.F.; Nolen, W.A.; Mendes, R.; Drexhage, H.A.; Burger, H. Feature-expression heat maps–A new visual method to explore complex associations between two variable sets. J. Biomed. Inform. 2015, 53, 156–161. [CrossRef] [PubMed]
Walkley, A.; Black, I.A. An examination of Degtjareff method for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Sci. 1934, 37, 29–37. [CrossRef]
