[en] Pseudomonas (P.) aeruginosa is the most frequently isolated Gram-negative bacteria in dog otitis. Antimicrobial
resistance is particularly prevalent in P. aeruginosa and phage therapy represents a promising alternative therapeutic strategy. The aim of this study was to assess the efficacy of the PEV2 phage against a clinical P. aeruginosa
isolate from a canine otitis using a Galleria (G.) mellonella larvae model. The genomic DNA of PAV237
P. aeruginosa isolate was sequenced and analysed. In a first main experiment, the efficacy of PEV2 phage against
PAV237 was assessed at different multiplicities of infection (MOI) (50,000, 5000, 500, 50) by analyzing the
larvae survival rate during 4 days. In a second experiment, the bacterial and phage titer evolutions were assessed
depending on two MOIs (50,000, 5000). No significant survival increase was observed with PEV2 therapy in the
infected larvae groups. The generated Kaplan-Meier curves showed that the rate of alive larvae was significantly
higher in the non-infected larvae compared to the infected-treated ones irrespective of phage MOIs. An increase
of the phage titer was observed at 24 and 48 h post-inoculation (HPI) with both MOIs and the P. aeruginosa titers
were lower with MOI 50,000 and 5000 compared to the infectivity control at 24 and 48 HPI. Even if an ineffectiveness of the PEV2 phage was observed on the larvae survival, PEV2 is active against P. aeruginosa in this
model and PEV2 replication is correlated with a lower bacterial proliferation in the phage treated larvae.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Antoine, Céline ; Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Gestion de la qualité dans la chaîne alimentaire
Laforêt, Fanny ; Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Gestion de la qualité dans la chaîne alimentaire
Blasdel, B.
Glonti, T.
Kutter, E.
Pirnay, J.P.
Mainil, Jacques ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Bactériologie et pathologie des maladies bactériennes
Delcenserie, Véronique ; Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Gestion de la qualité dans la chaîne alimentaire
Thiry, Damien ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Bactériologie et pathologie des maladies bactériennes
Language :
English
Title :
Efficacy assessment of PEV2 phage on Galleria mellonella larvae infected with a Pseudomonas aeruginosa dog otitis isolate
Publication date :
2021
Journal title :
Research in Veterinary Science
ISSN :
0034-5288
eISSN :
1532-2661
Publisher :
W. B. Saunders Co., United Kingdom
Volume :
136
Pages :
598-601
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
BioWin - BioWin : pôle santé de Wallonie [BE]
Funding text :
Service public de Wallonie. Projet BIOWIN : Inteliphages
Andrejko, M., Mizerska-Dudka, M., Jakubowicz, T., Antibacterial activity in vivo and in vitro in the hemolymph of Galleria mellonella infected with Pseudomonas aeruginosa. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 152 (2008), 118–123, 10.1016/j.cbpb.2008.10.008.
Arais, L.R., Barbosa, A.v., Carvalho, C.A., Cerqueira, A.M.F., Antimicrobial resistance, integron carriage, and gyrA and gyrB mutations in Pseudomonas aeruginosa isolated from dogs with otitis externa and pyoderma in Brazil. Vet. Dermatol. 27 (2016), 113–e31, 10.1111/vde.12290.
Barnard, N., Foster, A., How to treat Pseudomonas otitis in dogs. Vet. Rec. 182 (2018), 109–110, 10.1136/vr.k413.
Beeton, M.L., Alves, D.R., Enright, M.C., Jenkins, A.T.A., Assessing phage therapy against Pseudomonas aeruginosa using a Galleria mellonella infection model. Int. J. Antimicrob. Agents 46 (2015), 196–200, 10.1016/j.ijantimicag.2015.04.005.
Bernut, A., Belon, C., Soscia, C., Bleves, S., Blanc-Potard, A.B., Intracellular phase for an extracellular bacterial pathogen: MgtC shows the way. Microb. Cell 2 (2015), 353–355, 10.15698/mic2015.09.227.
Bourély, C., Cazeau, G., Jarrige, N., Leblond, A., Madec, J.Y., Haenni, M., Gay, E., Antimicrobial resistance patterns of bacteria isolated from dogs with otitis. Epidemiol. Infect. 147 (2019), 1–10, 10.1017/S0950268818003278.
Ceyssens, P.J., Brabban, A., Rogge, L., Lewis, M.S., Pickard, D., Goulding, D., Dougan, G., Noben, J.P., Kropinski, A., Kutter, E., Lavigne, R., Molecular and physiological analysis of three Pseudomonas aeruginosa phages belonging to the “N4-like viruses.”. Virology. 405 (2010), 26–30, 10.1016/j.virol.2010.06.011.
Fox, J., Using the R Commander: A Point-and-Click Interface for R. 2017, Chapman and Hall/CRC Press, Boca Raton FL.
Hall, A.R., de Vos, D., Friman, V.P., Pirnay, J.P., Buckling, A., Effects of sequential and simultaneous applications of phages on populations of Pseudomonas aeruginosa in vitro and in wax moth larvae. J. Appl. Environ. Microbiol. 78 (2012), 5646–5652, 10.1128/AEM.00757-12.
Hawkins, C., Harper, D., Burch, D., Änggård, E., Soothill, J., Topical treatment of Pseudomonas aeruginosa otitis of dogs with a phage mixture: a before/after clinical trial. Vet. Microbiol. 146 (2010), 309–313, 10.1016/j.vetmic.2010.05.014.
Hill, P.B., Lo, A., Eden, C.A.N., Huntley, S., Morey, V., Ramsey, S., Richardson, C., Smith, D.J., Sutton, C., Taylor, M.D., Thorpe, E., Tidmarsh, R., Williams, V., Survey of the prevalence, diagnosis and treatment of dermatological conditions in small animals in general practice. Vet. Rec., 158, 2006, 763, 10.1136/vr.158.22.763.
Hyun, J.E., Chung, T.H., Hwang, C.Y., Identification of VIM-2 metallo-β-lactamase-producing Pseudomonas aeruginosa isolated from dogs with pyoderma and otitis in Korea. Vet. Dermatol. 29 (2018), 186–e68, 10.1111/vde.12534.
Jeon, J., Dongeun, Y., Two novel phages improve survival in galleria mellonella infection and mouse acute pneumonia models infected with extensively drug-resistant Pseudomonas aeruginosa. J. Appl. Environ. Microbiol. 85 (2019), 1–16.
Ngassam-Tchamba, C., Duprez, J.N., Fergestad, M., de Visscher, A., L'Abee-Lund, T., de Vliegher, S., Wasteson, Y., Touzain, F., Blanchard, Y., Lavigne, R., Chanishvili, N., Cassart, D., Mainil, J., Thiry, D., In vitro and in vivo assessment of phage therapy against Staphylococcus aureus causing bovine mastitis. J. Glob. Antimicrob. Resist. 22 (2020), 762–770, 10.1016/j.jgar.2020.06.020.
Olszak, T., Zarnowiec, P., Kaca, W., Danis-Wlodarczyk, K., Augustyniak, D., Drevinek, P., de Soyza, A., McClean, S., Drulis-Kawa, Z., In vitro and in vivo antibacterial activity of environmental phages against Pseudomonas aeruginosa strains from cystic fibrosis patients. Appl. Microbiol. Biotechnol. 99 (2015), 6021–6033, 10.1007/s00253-015-6492-6.
Pirnay, J.P., Kutter, E., Bacteriophages: it's a medicine, Jim, but not as we know it. Lancet Infect. Dis. 3099 (2020), 1–3, 10.1016/S1473-3099(20)30464-3.
Thiry, D., Passet, V., Danis-Wlodarczyk, K., Lood, C., Wagemans, J., de Sordi, L., van Noort, V., Dufour, N., Debarbieux, L., Mainil, J.G., Brisse, S., Lavigne, R., New phages against emerging lineages ST23 and ST258 of Klebsiella pneumoniae and efficacy assessment in Galleria mellonella larvae. Viruses., 11, 2019, 411, 10.3390/v11050411.
Wang, L., Tkhilaishvili, T., Andres, B.B., Trampuz, A., Moreno, M.G., Bacteriophage-antibiotic combinations against ciprofloxacin/ceftriaxone-resistant Escherichia coli in vitro and in an experimental Galleria mellonella model. Int. J. Antimicrob. Agents, 56, 2020, 106200, 10.1016/j.ijantimicag.2020.106200.
Wright, A., Hawkins, C.H., Änggård, E.E., Harper, D.R., A controlled clinical trial of a therapeutic phage preparation in chronic otitis due to antibiotic-resistant Pseudomonas aeruginosa; a preliminary report of efficacy. Clin. Otolaryngol. 34 (2009), 349–357, 10.1111/j.1749-4486.2009.01973.x.
Zankari, E., Hasman, H., Cosentino, S., Vestergaard, M., Rasmussen, S., Lund, O., Aarestrup, F.M., Larsen, M.V., Identification of acquired antimicrobial resistance genes. J. Antimicrob. Chemother. 67 (2012), 2640–2644, 10.1093/jac/dks261.
