[en] A method of extraction of synthetic LH-RH is studied in human urines, using porous glass (Spherosil) and methanol. In the defined conditions the yield is greater than 80%. It appears that the method is reproducible. The recovery varies essentially with the quantity of Spherosil and the pH of methanol. The use of methanol acidified at pH 3 increases the speed and the importance of labelled LH-RH recovery.
Disciplines :
Endocrinology, metabolism & nutrition
Author, co-author :
Bourguignon, Jean-Pierre ; Université de Liège - ULiège > Département des sciences cliniques > Pédiatrie
Dourcy, C.
Franchimont, Paul
Language :
French
Title :
Mise au point de l'extraction du LH-RH dans les urines humaines : étude de l'extraction de l'hormone synthétique marquée
Alternative titles :
[en] Extraction of synthetic LH RH from human urine
Publication date :
1976
Journal title :
Comptes Rendus des Séances de la Société de Biologie et de ses Filiales
