Poster (Scientific congresses and symposiums)
AFM-based single molecule force spectroscopy on bimolecular hybrid DNA/RNA G-quadruplexes
Hubert, Sandrine; Gabelica, Valérie; Duwez, Anne-Sophie
20178th AFM Biomed Conference
 

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Abstract :
[en] Non-canonical nucleic acid structures, such as G-quadruplexes, have recently attracted significant research efforts for their potential roles in the regulation of numerous biological processes [1]. There is now evidence that these specific nucleic acid structures modulate gene expression. The formation of hybrid DNA/RNA G-quadruplexes (HQs) where each strand contribute to the structure with two series of guanines, was recently found to form in DNA during transcription [2]. It has been evidenced that the HQ formation competes with intramolecular G-quadruplexe motifs (DQ) and plays a major role in regulating transcription [3]. Given their very recent discovery, only a few studies on the formation of these hybrid structures have been conducted so far. However, the knowledge of their mechanism of formation and their detailed structure is important for understanding their biological function, as well as for manipulating gene expression by targeting HQs. We propose here to study these hybrid DNA/RNA G-quadruplexe structures by AFM-based single molecule force spectroscopy to obtain detailed information, at the single molecule level, on their formation mechanism and dynamics, their structure and interactions that govern the complex, their mechanical stability and their lability. References: [1] M. L. Bochman, K. Paeschke, V. A. Zakian, Nat. Rev. Genet., 13 (2012) 770. [2] K. W. Zheng, S. Xiao, J. Q. Liu, J. Y. Zhang, Y. H. Hao, Z. Tan, Nucleic Acids Res., 41 (2013) 5533. [3] R.-Y. Wu, K.-W Zheng, J.-Y. Zhang, Y.-H. Hao, Z. Tan, Angew. Chem. Int. Ed., 54 (2015) 2447.
Disciplines :
Chemistry
Author, co-author :
Hubert, Sandrine ;  Université de Liège - ULiège > Département de chimie (sciences) > Nanochimie et systèmes moléculaires
Gabelica, Valérie;  Institut Européen de Chimie et Biologie de Bordeaux
Duwez, Anne-Sophie  ;  Université de Liège - ULiège > Département de chimie (sciences) > Nanochimie et systèmes moléculaires
Language :
English
Title :
AFM-based single molecule force spectroscopy on bimolecular hybrid DNA/RNA G-quadruplexes
Publication date :
2017
Event name :
8th AFM Biomed Conference
Event place :
Cracovie, Poland
Event date :
du 4 septembre au 9 septembre 2017
Available on ORBi :
since 12 April 2021

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