Article (Scientific journals)
Modulation of IGF2 expression in the murine thymus and thymic epithelial cells following coxsackie-B4 infection
Michaux, Hélène; Halouani, Aymen; Trussart, Charlotte et al.
2021In Microorganisms, 9 (402), p. 1-21
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Keywords :
Thymus; Thymus epithelial cell; Type 1 diabetes; Coxsackievirus B4; Insulin-like growth factor 2; Transcription; Picornavirus
Abstract :
[en] Coxsackievirus B4 (CV-B4) can infect human and murine thymic epithelial cells (TECs). In a murine TEC cell line, CV-B4 can downregulate the transcription of the insulin-like growth factor 2 (Igf2) gene coding for the self-peptide of the insulin family. In this study, we show that CV-B4 infections of a murine TEC cell line decreased Igf2 P3 promoter activity by targeting a region near the transcription start site; however, the stability of Igf2 transcripts remained unchanged, indicating a regulation of Igf2 transcription. Furthermore, CV-B4 infections decreased STAT3 phosphorylation in vitro. We also showed that mice infected with CV-B4 had an altered expression of Igf2 isoforms as detected in TECs, followed by a decrease in the pro-IGF2 precursor in the thymus. Our study sheds new light on the intrathymic regulation of Igf2 transcription during CV-B4 infections and supports the hypothesis that a viral infection can disrupt central self-tolerance to insulin by decreasing Igf2 transcription in the thymic epithelium.
Research center :
GIGA-I3 - Giga-Infection, Immunity and Inflammation - ULiège
Disciplines :
Immunology & infectious disease
Endocrinology, metabolism & nutrition
Author, co-author :
Michaux, Hélène ;  University of Liege > GIGA-I3 > Neuroimmunoendocrinology
Halouani, Aymen ;  University of Liège > GIGA-I3 > Neuroimmunoendocrinology
Trussart, Charlotte ;  Université de Liège - ULiège > GIGA I3 - Immunoendocrinology
Jaïdane, Hela;  Université de Monastir
Martens, Henri ;  University of Liege > GIGA-I3 > Neuroimmunoendocrinology
Geenen, Vincent ;  Université de Liège - ULiège > Centre d'immunologie
Hober, Didier;  Université de Lille 2 > Laboratoire de Virologie
Language :
English
Title :
Modulation of IGF2 expression in the murine thymus and thymic epithelial cells following coxsackie-B4 infection
Publication date :
15 February 2021
Journal title :
Microorganisms
eISSN :
2076-2607
Publisher :
Molecular Diversity Preservation International (MDPI), Basel, Switzerland
Volume :
9
Issue :
402
Pages :
1-21
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
Fonds Léon Fredericq [BE]
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE]
Available on ORBi :
since 06 April 2021

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