Modulation of IGF2 expression in the murine thymus and thymic epithelial cells following coxsackie-B4 infection

[en] Coxsackievirus B4 (CV-B4) can infect human and murine thymic epithelial cells (TECs). In a murine TEC cell line, CV-B4 can downregulate the transcription of the insulin-like growth factor 2 (Igf2) gene coding for the self-peptide of the insulin family. In this study, we show that CV-B4 infections of a murine TEC cell line decreased Igf2 P3 promoter activity by targeting a region near the transcription start site; however, the stability of Igf2 transcripts remained unchanged, indicating a regulation of Igf2 transcription. Furthermore, CV-B4 infections decreased STAT3 phosphorylation in vitro. We also showed that mice infected with CV-B4 had an altered expression of Igf2 isoforms as detected in TECs, followed by a decrease in the pro-IGF2 precursor in the thymus. Our study sheds new light on the intrathymic regulation of Igf2 transcription during CV-B4 infections and supports the hypothesis that a viral infection can disrupt central self-tolerance to insulin by decreasing Igf2 transcription in the thymic epithelium.

Research Center/Unit :

GIGA-I3 - Giga-Infection, Immunity and Inflammation - ULiège

Disciplines :

Immunology & infectious disease
Endocrinology, metabolism & nutrition

Author, co-author :

Michaux, Hélène ; University of Liege > GIGA-I3 > Neuroimmunoendocrinology

Halouani, Aymen ; University of Liège > GIGA-I3 > Neuroimmunoendocrinology

Trussart, Charlotte ; Université de Liège - ULiège > GIGA I3 - Immunoendocrinology

Jaïdane, Hela; Université de Monastir

Martens, Henri ; University of Liege > GIGA-I3 > Neuroimmunoendocrinology

Geenen, Vincent ; Université de Liège - ULiège > Centre d'immunologie

Hober, Didier; Université de Lille 2 > Laboratoire de Virologie

Language :

English

Title :

Modulation of IGF2 expression in the murine thymus and thymic epithelial cells following coxsackie-B4 infection

Publication date :

15 February 2021

Journal title :

Microorganisms

eISSN :

2076-2607

Publisher :

Molecular Diversity Preservation International (MDPI), Basel, Switzerland

Volume :

Issue :

402

Pages :

1-21

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

Fonds Léon Fredericq [BE]
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE]

Available on ORBi :

since 06 April 2021

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