[en] Circular RNAs (circRNAs) are a newly discovered class of endogenous non-coding RNAs that play an important role in growth and development by regulating gene expression and participating in a variety of biological processes. However, the role of circRNAs in porcine follicles remains unclear. Therefore, this study examined middle-sized ovarian follicles obtained from Meishan and Duroc sows at day 4 of the follicular phase. High-throughput RNA sequencing (RNA-seq) was utilized to construct circRNAs, and differential expression was identified. The findings were validated using reverse transcription PCR (RT-PCR) and DNA sequencing, GO and KEGG analyses were performed, and potential miRNA targets were identified. The RNA-seq identified a total of 15,866 circRNAs, with 244 differentially expressed in the Meishan relative to the Duroc (111 up-regulated and 133 down-regulated). The RT-PCR finding confirmed the RNA-seq results, and quantitative real-time PCR (qPCR) analysis examining a subset of the circRNAs showed that they are resistant to RNase R digestion. Bioinformatics analysis (GO and KEGG) showed that the host genes associated with the differentially expressed circRNAs are involved in reproduction and follicular development signaling pathways. Furthermore, many of the circRNAs were found to interact with miRNAs that are associated with follicular development. This study presents a new perspective for studying circRNAs and provides a valuable resource for further examination into the potential roles of circRNAs in porcine follicular development.
Research Center/Unit :
College of Animal Science and Technology, Shihezi University, 221 North Fourth Road, Shihezi, 832000 China Key Laboratory of Animal Breeding and Reproduction of Minstry of Education,College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070 China University of Liège, Gembloux Agro-Bio Tech (ULiège-GxABT), Gembloux, Belgium
Disciplines :
Animal production & animal husbandry
Author, co-author :
Xie, Su; Shihezi University > College of Animal Science and Technology
Huang, Tao; Shihezi University > College of Animal Science and Technology,
Critical editor :
Li, Mengxun; Shihezi University > College of Animal Science and Technology
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