[en] In this study, cattle bone collagen peptides (CPs) were applied to prepare peptides-calcium chelate (CPs-Ca), and the physicochemical, structural properties, and stability of the CPs-Ca were characterized. The optimized preparation conditions were determined by single factor test, and the maximum calcium-chelating capacity was 42.70 ± 1.09 μg/mg. Physicochemical analysis indicated that CPs-Ca was a new substance compared with CPs. Structural analysis demonstrated that calcium ions chelated CPs via the sites of carboxyl oxygen, hydroxyl oxygen and amino nitrogen atom. Morphology analysis showed that CPs-Ca aggregated to large particles due to the crosslinking of calcium ions. According to the stability analysis, CPs-Ca was stable in thermal processing and gastrointestinal digestion. The acid environment was the major factor of CPs-Ca dissociation. The findings put forward a scientific basis for preparing a novel calcium supplement and high-value utilization of cattle bone.
Disciplines :
Chemistry
Author, co-author :
Zhang, Hongru; Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China
Zhao, Laiyu; Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China
Shen, Qingshan; Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China
Qi, Liwei; Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China
Jiang, Shan
Guo, Yujie; Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China
Zhang, Chunhui; Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China
Richel, Aurore ; Université de Liège - ULiège > Département GxABT > SMARTECH
Language :
English
Title :
Preparation of cattle bone collagen peptides-calcium chelate and its structural characterization and stability
Balk, E.M., Adam, G.P., Langberg, V.N., Earley, A., Clark, P., Ebeling, P.R., Global dietary calcium intake among adults: A systematic review. Osteoporosis International 28:12 (2017), 3315–3324, 10.1007/s00198-017-4230-x.
Chen, K., Cheng, W., Li, M., Yang, D., Lin, S., Calcification of senile cataractous lens determined by Fourier transform infrared (FTIR) and Raman microspectroscopies. Journal of Microscopy 219:1 (2005), 36–41, 10.1111/j.1365-2818.2005.01491.x.
Cui, P., Lin, S., Han, W., Jiang, P., Zhu, B., Sun, N., Calcium delivery system Assembled by a nanostructured peptide derived from the sea cucumber ovum. Journal of Agricultural and Food Chemistry 67:44 (2019), 12283–12292, 10.1021/acs.jafc.9b04522.
Cui, P., Sun, N., Jiang, P., Wang, D., Lin, S., Optimised condition for preparing sea cucumber ovum hydrolysate–calcium complex and its structural analysis. International Journal of Food Science and Technology 52:8 (2017), 1914–1922, 10.1111/ijfs.13468.
De Barboza, G.D., Guizzardi, S., De Talamoni, N.T., Molecular aspects of intestinal calcium absorption. World Journal of Gastroenterology 21:23 (2015), 7142–7154, 10.3748/wjg.v21.i23.7142.
Felix, B., Pansu, D., Nutritional aspects of calcium absorption. Journal of Nutrition 129:1 (1999), 9–12, 10.1093/jn/129.1.9.
Hou, T., Liu, Y., Guo, D., Li, B., He, H., Collagen peptides from crucian skin improve calcium bioavailability and structure characterization by HPLC-ESI-MS/MS. Journal of Agricultural and Food Chemistry 65:40 (2017), 8847–8854, 10.1021/acs.jafc.7b03059.
Hou, T., Wang, C., Ma, Z., Shi, W., Weiwei, L., He, H., Desalted duck egg white peptides: Promotion of calcium uptake and structure characterization. Journal of Agricultural and Food Chemistry, 2015, 8170–8176, 10.1021/acs.jafc.5b03097.
Hou, H., Wang, S., Zhu, X., Li, Q., Fan, Y., Cheng, D., et al. A novel calcium-binding peptide from Antarctic krill protein hydrolysates and identification of binding sites of calcium-peptide complex. Food Chemistry 243 (2018), 389–395, 10.1016/j.foodchem.2017.09.152.
Hung, Y., Verbeke, W., Sensory attributes shaping consumers' willingness-to-pay for newly developed processed meat products with natural compounds and a reduced level of nitrite. Food Quality and Preference 70 (2018), 21–31, 10.1016/j.foodqual.2017.02.017.
Liao, W., Chen, H., Jin, W., Yang, Z., Cao, Y., Miao, J., Three newly isolated calcium-chelating peptides from Tilapia bone collagen hydrolysate enhance calcium absorption activity in intestinal caco-2 cells. Journal of Agricultural and Food Chemistry 68:7 (2020), 2091–2098, 10.1021/acs.jafc.9b07602.
Lin, Cai, X., Tang, M., Wang, S., Preparation and evaluation of the chelating nanocomposite fabricated with marine algae schizochytrium sp. protein hydrolysate and calcium. Journal of Agricultural and Food Chemistry 63:44 (2015), 9704–9714, 10.1021/acs.jafc.5b04001.
Lin, Cai, X., Wu, X., Lin, S., Wang, S., Fabrication of snapper fish scales protein hydrolysate-calcium complex and the promotion in calcium cellular uptake. Journal of Functional Foods, 65, 2020, 103717, 10.1016/j.jff.2019.103717.
Osborne, C.G., Mctyre, R.B., Dudek, J., Roche, K.E., Scheuplein, R., Silverstein, B., Evidence for the relationship of calcium to blood pressure. Nutrition Reviews 54:12 (2010), 365–381, 10.1111/j.1753-4887.1996.tb03850.x.
Peacock, M., Calcium metabolism in health and disease. Clinical Journal of the American Society of Nephrology 5:1 (2010), 881–898, 10.2215/CJN.05910809.
Sánchez-Rivera, L., Martínez-Maqueda, D., Cruz-Huerta, E., Miralles, B., Recio, I., Peptidomics for discovery, bioavailability and monitoring of dairy bioactive peptides. Food Research International 63 (2014), 170–181, 10.1016/j.foodres.2014.01.069.
Shen, Q., Zhang, C., Jia, W., Qin, X., Cui, Z., Mo, H., et al. Co-production of chondroitin sulfate and peptide from liquefied chicken sternal cartilage by hot-pressure. Carbohydrate Polymers, 222, 2019, 115015, 10.1016/j.carbpol.2019.115015.
Straub, D.A., Calcium supplementation in clinical practice: A review of forms, doses, and indications. Nutrition in Clinical Practice 22:3 (2007), 286–296, 10.1177/0115426507022003286.
Sun, N., Cui, P., Lin, S., Yu, C., Tang, Y., Wu, H., Characterization of sea cucumber (stichopus japonicus) ovum hydrolysates: Calcium chelation, solubility and absorption into intestinal epithelial cells. Journal of the Science of Food and Agriculture 97:13 (2017), 4604–4611, 10.1002/jsfa.8330.
Vavrusova, M., Skibsted, L.H., Calcium nutrition. Bioavailability and fortification. LWT-Food Science and Technology 59:2 (2014), 1198–1204, 10.1016/j.lwt.2014.04.034.
Wang, L., Ding, Y., Zhang, X., Li, Y., Wang, R., Chen, Z., Isolation of a novel calcium-binding peptide from wheat germ protein hydrolysates and the prediction for its mechanism of combination. Food Chemistry 239 (2018), 416–426, 10.1016/j.foodchem.2017.06.090.
Wang, J., Zhang, B., Lu, W., Liu, J., Guo, Y., Cell proliferation stimulation ability and osteogenic activity of low molecular weight peptides derived from bovine gelatin hydrolysates. Journal of Agricultural and Food Chemistry 68:29 (2020), 7630–7640, 10.1021/acs.jafc.0c02717.
Wang, X., Zhang, Z., Xu, H., Li, X., Hao, X., Preparation of sheep bone collagen peptide–calcium chelate using enzymolysis-fermentation methodology and its structural characterization and stability analysis. RSC Advances 10:20 (2020), 11624–11633, 10.1039/d0ra00425a.
Wu, W., He, L., Liang, Y., Yue, L., Peng, W., Jin, G., et al. Preparation process optimization of pig bone collagen peptide-calcium chelate using response surface methodology and its structural characterization and stability analysis. Food Chemistry 284 (2019), 80–89, 10.1016/j.foodchem.2019.01.103.
Yang, X., Yu, X., Yagoub, A.-G., Chen, L., Wahia, H., Osae, R., et al. Structure and stability of low molecular weight collagen peptide (prepared from white carp skin) -calcium complex. LWT-Food Science and Technology, 136, 2021, 110335, 10.1016/j.lwt.2020.110335.
Ye, M., Jia, W., Zhang, C., Shen, Q., Zhu, L., Wang, L., Preparation, identification and molecular docking study of novel osteoblast proliferation-promoting peptides from yak (Bos grunniens) bones. RSC Advances 9:26 (2019), 14627–14637, 10.1039/c9ra00945k.
Zhang, K., Li, B., Chen, Q., Zhang, Z., Zhao, X., Hou, H., Functional calcium binding peptides from pacific cod (Gadus macrocephalus) bone: Calcium bioavailability enhancing activity and anti-osteoporosis effects in the ovariectomy-induced osteoporosis rat model. Nutrients, 10(9), 2018, 1325, 10.3390/nu10091325.
Zhao, N., Hu, J., Hou, T., Ma, Z., Wang, C., He, H., Effects of desalted duck egg white peptides and their products on calcium absorption in rats. Journal of Functional Foods 8 (2014), 234–242, 10.1016/j.jff.2014.03.022.
