Antimicrobial susceptibility of mycoplasma bovis isolates from veal, dairy and beef herds

Epidemiological cut-off methods; Gamithromycin; Genomic clusters; Iterative statistical method; Normalized resistance interpretation; Visual estimation

Abstract :

[en] Mycoplasma bovis is an important pathogen causing mostly pneumonia in calves and mastitis in dairy cattle. In the absence of an effective vaccine, antimicrobial therapy remains the main control measure. Antimicrobial use in veal calves is substantially higher than in conventional herds, but whether veal calves also harbor more resistant M. bovis strains is currently unknown. Therefore, we compared antimicrobial susceptibility test results of M. bovis isolates from different cattle sectors and genomic clusters. The minimum inhibitory concentration of nine antimicrobials was determined for 141 Belgian M. bovis isolates (29 dairy, 69 beef, 12 mixed, 31 veal farms), and was used to estimate the epidemiological cut-off. Acquired resistance was frequently observed for the macrolides, while no acquired resistance to oxytetracycline and doxycycline, minimal acquired resistance to florfenicol and tiamulin, and a limited acquired resistance to enrofloxacin was seen. M. bovis isolates from beef cattle or genomic cluster III had higher odds of being gamithromycin-resistant than those from dairy cattle or genomic clusters IV and V. In this study, no cattle industry could be identified as source of resistant M. bovis strains. A single guideline for antimicrobial use for M. bovis infections, with a small remark for gamithromycin, is likely sufficient. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Bokma, J.; Department of Large Animal Internal Medicine, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, Merelbeke, 9820, Belgium, Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, Merelbeke, 9820, Belgium

Gille, Linde ; Université de Liège - ULiège > CVU : pôle ruminants-porcs

De Bleecker, K.; Animal Health Service-Flanders, Industrielaan 29, Torhout, 8820, Belgium

Callens, J.; Animal Health Service-Flanders, Industrielaan 29, Torhout, 8820, Belgium

Haesebrouck, F.; Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, Merelbeke, 9820, Belgium

Pardon, B.; Department of Large Animal Internal Medicine, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, Merelbeke, 9820, Belgium

Boyen, F.; Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, Merelbeke, 9820, Belgium

Language :

English

Title :

Antimicrobial susceptibility of mycoplasma bovis isolates from veal, dairy and beef herds

Publication date :

2020

Journal title :

Antibiotics

eISSN :

2079-6382

Publisher :

MDPI AG

Volume :

Issue :

Pages :

1-12

Peer reviewed :

Peer Reviewed verified by ORBi

Name of the research project :

Grant number RF17/6313 (MALDIRESP); DGZ Vlaanderen Veepeiler Project 2018; Hercules project G0H2516N (AUGE/15/05)

Funders :

SPF Santé - Service Public Fédéral Santé publique. Sécurité de la Chaîne alimentaire et Environnement [BE]
FWO - Fonds Wetenschappelijk Onderzoek Vlaanderen [BE]

Available on ORBi :

since 15 February 2021

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