[en] Lawsonia intracellularis (LI) is an obligate intracellular gram-negative rod causing equine proliferative enteropathy (EPE). Occasional cases of EPE have been reported in foals living in Belgium, but the seroprevalence of equine LI in this country is unknown.
The target population included clinically healthy adult horses, whose blood samples were collected and analysed for specific IgG antibodies against LI using a blocking enzyme-linked immunosorbent assay (bELISA) test. Results were expressed as Percentage of Inhibition (PI). Samples that had a PI <20% were judged as negative, those between 20 and 30% as inconclusive and those >30% were considered positive.
A total of 356 blood samples were analysed with 352 horses (98.8%) testing positive, 2 horses (0. 6%) testing negative and 2 horses (0.6%) showing inconclusive results. The large percentage of seropositive samples obtained in this study confirms a widespread exposure of Belgian horses to LI.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Loublier, Clémence ; Université de Liège - ULiège > Dép. clinique des animaux de compagnie et des équidés (DCA) > Dép. clinique des animaux de compagnie et des équidés (DCA)
Cerri, Simona
Gryspeerdt, Annick; Animal Health Care Flanders (DGZ) - site Torhout, Torhout, Belgium.
Amory, Hélène ; Université de Liège - ULiège > Dép. clinique des animaux de compagnie et des équidés (DCA) > Médecine interne des équidés
Bauwens, Caroline; Faculty of Veterinary Medicine, Department of Large Animal Internal Medicine, Ghent University, Merelbeke, Belgium.
Cesarini Latorre, Carlota ; Université de Liège - ULiège > Dép. clinique des animaux de compagnie et des équidés (DCA) > Médecine interne des équidés
Language :
English
Title :
High Seroprevalence Against Lawsonia intracellularis Among Adult Horses in Belgium
Deprez, K., Chiers, C., Gebhart, J., Ducatelle, R., Lefère, L., Vanschandevijl, K., et al. Lawsonia intracellularis infection in a 12-month-old colt in Belgium. Vet Rec 157 (2005), 774–776.
Gebhart, C.J., Proceedings of the 19th International Pig Veterinary Society Congress; 16-19 July 2006, Copenhagen, Denmark Lawsonia intracellularis infections, vol. 1, 2006, 48–53.
Smith, D.G., Lawson, G.H., Lawsonia intracellularis: getting inside the pathogenesis of proliferative enteropathy. Vet Microbiol 82 (2001), 331–345.
Bihr, T.P., Protein-losing enteropathy caused by Lawsonia intracellularis in a weanling foal. Can Vet J 44 (2003), 65–66.
Feary, D.J., Hassel, D.M., Enteritis and colitis in horses. Vet Clin North Am Equine Pract 22 (2006), 437–479.
Lavoie, J.P., Drolet, R., Parsons, D., Leguillette, R., Sauvageau, R., Shapiro, J., et al. Equine proliferative enteropathy: a cause of weight loss, colic, diarrhoea and hypoproteinaemia in foals on three breeding farms in Canada. Equine Vet J 32 (2000), 418–425.
Schumacher, J., Schumacher, J., Rolsma, M., Brock, K.V., Gebhart, C.J., Surgical and medical treatment of an Arabian filly with Proliferative Enteropathy caused by Lawsonia intracellularis. J Vet Intern Med 14 (2000), 630–632.
McGurrin, M.K., Vengust, M., Arroyo, L.G., et al. An outbreak of Lawsonia intracellularis infection in a standardbred herd in Ontario. Can Vet J 48 (2008), 927–930.
Merlo, J.L., Sheats, M.K., Elce, Y., et al. Outbreak of Lawsonia intracellularis on a standardbred farm in North Carolina. Equine Vet Educ 21 (2009), 179–182.
Pusterla, N., Gebhart, C., Lawsonia intracellularis infection and proliferative enteropathy in foals. Vet Microbiol 167 (2013), 34–41.
Allen, K.J., Pearson, G.R., Fews, D., McOrist, S., Brazil, T.J., Lawsonia intracellularis proliferative enteropathy in a weanling foal, with a tentative histological diagnosis of lymphocytic plasmacytic enteritis. Equine Vet Educ 21 (2009), 411–414.
Breuer, J., Schmoll, F., Uhlig, A., Schusser, G.F., A follow up study on antibodies against Lawsonia intracellularis in mares and foals from two breeding farms in Germany. Berl Munch Tierarztl Wochenschr 124 (2011), 337–342.
Van Den Wollenberg, L., Butler, C.M., Houwers, D.J., et al. Lawsonia intracellularis-associated proliferative enteritis in weanling foals in The Netherlands. Tijdschr Diergeneeskd 136 (2011), 565–570.
Bohlin, A.M., Olsen, S.N., Laursen, S.H., Ohman, A., Van Galen, G., Lawsonia intracellularis associated equine proliferative enteropathy in Danish weanling foals. Acta Vet Scand, 61, 2019, 12.
Wuersch, K., Huessy, D., Koch, C., Oevermann, A., Lawsonia intracellularis proliferative enteropathy in a filly. J Vet Med A 53 (2006), 17–21.
Gabardo, M.P., Sato, J.P.H., Resende, T.P., Guedes, R.M.C., Equine proliferative enteropathy on a Brazilian farm. Pesq Vet Bras 35 (2015), 443–447.
McClintock, S.A., Collins, A.M., Lawsonia intracellularis proliferative enteropathy in a weanling foal in Australia. Aust Vet J 82 (2004), 750–752.
Shimizu, C., Shibahara, T., Takai, S., Kasuyax, K., Chikubax, T., Murakoshix, N., et al. Lawsonia intracellularis and virulent rhodococcus equi infection in a thoroughbred. J Comp Pathol 143 (2010), 303–308.
Frazer, M.L., Lawsonia intracellularis infection in horses: 2005–2007. J Vet Intern Med 22 (2008), 1243–1248.
Page, A.E., Stills, H.F., Chander, Y., Gebhart, C.J., Horohov, D.W., Adaptation and validation of a bacteria specific enzyme-linked immunosorbent assay for determination of farm-specific Lawsonia intracellularis seroprevalence in central Kentucky Thoroughbreds. Equine Vet J 43 (2011), 25–31.
Jacobson, M., Aspanb, A., Königsson, M.H., Segerstad, C.H., Wallgren, P., Fellström, C., et al. Routine diagnostics of Lawsonia intracellularis performed by PCR, serological and post mortem examination, with special emphasis on sample preparation methods for PCR. Vet Microbiol 102 (2004), 189–201.
Guedes, R.M., Update on epidemiology and diagnosis of porcine proliferative enteropathy. J Swine Health Prod 12 (2004), 134–138.
Guedes, R.M., Gebhart, C.J., Deen, J., Winkelman, N.L., Validation of an immunoperoxidase monolayer assay as a serologic test for porcine proliferative enteropathy. J Vet Diagn Invest 14 (2002), 528–530.
Boesen, H.T., Jensen, T.K., Moller, K., Nielsen, L.H., Jungersen, G., Evaluation of a novel enzyme-linked immunosorbent assay for serological diagnosis of porcine proliferative enteropathy. Vet Microbiol 109 (2005), 105–112.
Wattanaphansak, S., Asawakarn, T., Gebhart, C.J., Deen, J., Development and validation of an enzyme-linked immunosorbent assay for the diagnosis of porcine proliferative enteropathy. J Vet Diagn Invest 20 (2008), 170–177.
Slovis, N., Management of equine proliferative enteropathy. Practice 38 (2014), 287–292.
Keller, C., Schroeder, H., Ambiguous clinical signs demands diagnosis by a laboratory. Pig Prog, 21, 2005, 10.
Lebret, A., Auvigne, V., Saives, Morel, A Longitudinal evaluation of three commercial diagnostic assays for Lawsonia intracellularis infection in pigs. Vet Rec, 172, 2013, 341.
Hassenin, A.S.H., Sena, S., Gebhart, C.J., Goyal, S.M., Comparison of ELISA and IPMA tests for the detection of Anti-Lawsonia antibodies in horses. Int J Adv Res Technol 6 (2017), 17–24.
Kranenburg, L.C., Van Ree, H.E., Calis, A.N., de Pater, M., Buter, G.J., van Maanen, C., et al. The seroprevalence of Lawsonia intracellularis in horses in The Netherlands. Tijdschr Diergeneeskd 136 (2011), 237–243.
Guimarães-Ladeira, C.V., Palhares, M.S., Oliveira, J.S., Ramirez, M.A., Guedes, R.M., Faecal shedding and serological cross-sectional study of Lawsonia intracellularis in horses in the state of Minas Gerais, Brazil. Equine Vet J 41 (2009), 593–596.
Steimnan, A., Aharonson-Raz, K., Blum S, E., Shnaiderman, A., Klement, E., Lensky, I.M., et al. Demographic and environmental risk factors for exposure to Lawsonia intracellularis in horses in Israel. J Equine Vet Sci 34 (2014), 641–646.
Page, A.E., Stills, H., Horohov, D.W., The effect of passively acquired antibodies on Lawsonia intracellularis infection and immunity in the horse. Equine Vet J 47 (2014), 655–661.
Pusterla, N., Higgins, J.C., Smith, P., Mapes, S., Epidemiological survey on farms with documented occurrence of equine proliferative enteropathy due to Lawsonia intracellularis. Vet Rec 163 (2008), 156–158.
