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Corneal preparation of eye bank eyes for experimental surgery.
Hamaoui, M.; Tahi, H.; Chapon, P. et al.
2001In Cornea, 20 (3), p. 317-20
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Keywords :
Acetates/pharmacology; Aged; Aged, 80 and over; Cornea/drug effects/surgery; Desiccation; Dextrans/pharmacology; Drug Combinations; Eye Banks; Humans; Middle Aged; Minerals/pharmacology; Ophthalmologic Surgical Procedures; Sodium Chloride/pharmacology; Specimen Handling/methods; Tissue Donors
Abstract :
[en] PURPOSE: To evaluate the efficacy of dextran in balanced salt solution (BSS) as a preparation of eye bank corneas for experimental surgeries. METHODS: We used 12 eye bank eyes that were unsuitable for transplant. The corneas were removed from the globe and affixed to a Hanna artificial anterior chamber. Four concentrations of dextran-BSS (10%, 15%, 20%, and 30% [+/-0.56%]) were used to dehydrate the corneas, and corneal thickness was assessed with an ultrasonic pachymeter (+/-5 microm) at varying time-intervals over 3 hours. RESULTS: The corneas were thinned to an average thickness of 477+/-54 microm, 430+/-54 microm, 406+/-61 microm, and 391+/-52 microm at average times of 75+/-0 minutes, 85+/-23 minutes, 60+/-12 minutes, and 45+/-0 minutes for the 10%, 15%, 20%, and 30% concentrations, respectively. Corneal thickness was stabilized in both a 15% and a 20% solution. The data was fit to exponential curves until a minimum value was reached, after which linear regression analysis was used to determine the slopes of the data. The slopes for the 15% and 20% concentrations were not significantly different from 0 (p = 0.5 and p = 0.4, respectively). The slopes for each set of data from the 10% and 30% concentrations were significantly different from each other (p = 0.010 and p = 0.001, respectively). CONCLUSIONS: A solution of 20% dextran in BSS is effective for dehydrating eye bank corneas to a quasi-normal physiologic thickness and for maintaining the thickness for a sufficient amount of time. Both the posterior and anterior sides of the cornea should be exposed to the solution for 60 minutes.
Disciplines :
Ophthalmology
Author, co-author :
Hamaoui, M.
Tahi, H.
Chapon, P.
Duchesne, Bernard ;  Université de Liège - ULiège > Département des sciences cliniques > Département des sciences cliniques
Fantes, F.
Feuer, W.
Parel, J. M.
Language :
English
Title :
Corneal preparation of eye bank eyes for experimental surgery.
Publication date :
2001
Journal title :
Cornea
ISSN :
0277-3740
eISSN :
1536-4798
Publisher :
Lippincott Williams & Wilkins, United States - Maryland
Volume :
20
Issue :
3
Pages :
317-20
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 29 January 2021

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