Article (Scientific journals)
The Trypanosoma Brucei KIFC1 Kinesin Ensures the Fast Antibody Clearance Required for Parasite Infectivity.
Lecordier, Laurence; Uzureau, Sophie; Vanwalleghem, Gilles et al.
2020In iScience, 23 (9), p. 101476
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Keywords :
Cell Biology; Immunology; Microbiology Parasite
Abstract :
[en] Human innate immunity to Trypanosoma brucei involves the trypanosome C-terminal kinesin TbKIFC1, which transports internalized trypanolytic factor apolipoprotein L1 (APOL1) within the parasite. We show that TbKIFC1 preferentially associates with cholesterol-containing membranes and is indispensable for mammalian infectivity. Knockdown of TbKIFC1 did not affect trypanosome growth in vitro but rendered the parasites unable to infect mice unless antibody synthesis was compromised. Surface clearance of Variant Surface Glycoprotein (VSG)-antibody complexes was far slower in these cells, which were more susceptible to capture by macrophages. This phenotype was not due to defects in VSG expression or trafficking but to decreased VSG mobility in a less fluid, stiffer surface membrane. This change can be attributed to increased cholesterol level in the surface membrane in TbKIFC1 knockdown cells. Clearance of surface-bound antibodies by T. brucei is therefore essential for infectivity and depends on high membrane fluidity maintained by the cholesterol-trafficking activity of TbKIFC1.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Lecordier, Laurence
Uzureau, Sophie
Vanwalleghem, Gilles
Deleu, Magali  ;  Université de Liège - ULiège > Département GxABT > Chimie des agro-biosystèmes
Crowet, Jean-Marc 
Barry, Paul
Moran, Barry
Voorheis, Paul
Dumitru, Andra-Cristina
Yamaryo-Botté, Yoshiki
Dieu, Marc
Tebabi, Patricia
Vanhollebeke, Benoit
Lins, Laurence  ;  Université de Liège - ULiège > Département GxABT > Chimie des agro-biosystèmes
Botté, Cyrille Y.
Alsteens, David
Dufrêne, Yves
Pérez-Morga, David
Nolan, Derek P.
Pays, Etienne
More authors (10 more) Less
Language :
English
Title :
The Trypanosoma Brucei KIFC1 Kinesin Ensures the Fast Antibody Clearance Required for Parasite Infectivity.
Publication date :
2020
Journal title :
iScience
eISSN :
2589-0042
Publisher :
Elsevier, Cambridge, United States - Massachusetts
Volume :
23
Issue :
9
Pages :
101476
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.
Available on ORBi :
since 26 January 2021

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