Reference : Radioimmunoassay of Substance P in Lung Perfusate
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
Life sciences : Veterinary medicine & animal health
Radioimmunoassay of Substance P in Lung Perfusate
Nemmar, A. [Université de Liège - ULg > > > > > >]
Gustin, Pascal mailto [Université de Liège - ULg > Département de sciences fonctionnelles > Pharmacologie, pharmacothérapie et toxicologie >]
Delaunois, Annie mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Beckers, Jean-François mailto [Université de Liège - ULg > Département de sciences fonctionnelles > Physiologie de la reproduction >]
Sulon, Joseph [Université de Liège - ULg > Département de sciences fonctionnelles > Physiologie de la reproduction >]
Journal of Pharmacological & Toxicological Methods
Elsevier Science
Yes (verified by ORBi)
[en] Tyrosine/diagnostic use ; Substance P/pharmacokinetics ; Sensitivity and Specificity ; Reproducibility of Results ; Radioimmunoassay/methods ; Rabbits ; Perfusion/methods ; Lung/drug effects/metabolism ; Capsaicin/pharmacology ; Animals
[en] A radioimmunoassay of the undecapeptide substance P (SP) and its application in rabbit lung superfusate has been developed. The assay was based on the use of a radioactive tracer, 125I-SP-Tyr8, which showed, with an excess of antibodies, a specific binding greater than 90% and a nonspecific binding lower than 2%. This tracer was stable for 4 weeks at -20 degrees C. Its specific radioactivity was 384 Ci/mmol. The assay's lower limit of detection was 10 pg/ml (0.7 fmol). The determination of SP in rabbit lung perfusate required extraction and concentration using octadecylsilane cartridges. Under these conditions, the recovery of SP from experiments on lung perfusates (n=4) was 75.0+/-4.5%, and the intraassay and interassay coefficients of variation were 7.3+/-1.9% and 10.7+/-1.3%, respectively. Amounts of SP released in rabbit lung perfusates were determined following stimulation of pulmonary C fibers with capsaicin (10(-4) M). During the stimulation period, the values of SP from lung perfusates (n=6) increased significantly when compared with baseline values.
Researchers ; Professionals

File(s) associated to this reference

Fulltext file(s):

Restricted access
NemmarA-JPharmacolToxicolMethods-1998.pdfPublisher postprint579.29 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.