Article (Scientific journals)
Chronic hyperosmotic stress interferes with immune homeostasis in striped catfish (Pangasianodon hypophthalmus, S.) and leads to excessive inflammatory response during bacterial infection
Schmitz, M.; Douxfils, J.; Mandiki, S. N. M. et al.
2016In Fish and Shellfish Immunology, 55, p. 550-558
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Keywords :
Hyperosmotic stress; Immunity; Osmolality; Striped catfish; Edwardsiella ictaluri; Enterobacteriaceae infection; Animals; Catfishes; Enterobacteriaceae Infections; Fish Diseases; Fish Proteins; HMGB1 Protein; Homeostasis; HSP70 Heat-Shock Proteins; Immunity, Innate; Inflammation; Molecular Chaperones; Osmotic Pressure; Sepsis
Abstract :
[en] Hyperosmotic stress has often been investigated from osmoregulation perspectives while the effects of such stress on the immune capacity remain largely unexplored. In this study, striped catfish were submitted to three salinity profiles (freshwater, low saline water, saline water) during 20 days, followed by infection with a virulent bacteria, Edwardsiella ictaluri, responsible for the enteric septicaemia of catfish. Osmoregulatory (plasma osmolality, gill Na+K+ATPase), immune (blood cells, lysozyme activity, complement activity, respiratory burst) parameters and mortality rate were investigated. In addition, abundances of heat shock protein 70 and high mobility group box 1 were explored. With elevated salinity, plasma osmolality severely increased while gill Na+K+ATPase slightly increased. Salinity alone stimulated the number of granulocytes, lysozyme activity and respiratory burst but depleted the number of thrombocytes. Salinity in combination with infection stimulated the number of monocytes and ACH50. On the contrary, erythrocytes, hematocrit, heat shock protein 70 and high mobility group box 1 did not significantly vary with salinity profiles. Then, salinity induced earlier onset on mortalities after E. ictaluri inoculation whereas cumulative mortality reach 79.2%, 67.0% and 91.7% respectively in freshwater, low saline water and saline water. In conclusion, salinity stimulates several immune functions in striped catfish but prolonged exposure to excessive hyperosmotic condition may lead to excessive inflammatory response and death. © 2016 Elsevier Ltd
Disciplines :
Zoology
Author, co-author :
Schmitz, M.;  University of Namur, Research Unit in Environmental and Evolutionary Biology, Namur, Belgium
Douxfils, J.;  University of Namur, Research Unit in Environmental and Evolutionary Biology, Namur, Belgium
Mandiki, S. N. M.;  University of Namur, Research Unit in Environmental and Evolutionary Biology, Namur, Belgium
Morana, Cédric ;  Université de Liège - ULiège > Département d'astrophys., géophysique et océanographie (AGO) > Chemical Oceanography Unit (COU)
Baekelandt, S.;  University of Namur, Research Unit in Environmental and Evolutionary Biology, Namur, Belgium
Kestemont, P.;  University of Namur, Research Unit in Environmental and Evolutionary Biology, Namur, Belgium
Language :
English
Title :
Chronic hyperosmotic stress interferes with immune homeostasis in striped catfish (Pangasianodon hypophthalmus, S.) and leads to excessive inflammatory response during bacterial infection
Publication date :
2016
Journal title :
Fish and Shellfish Immunology
ISSN :
1050-4648
eISSN :
1095-9947
Publisher :
Academic Press
Volume :
55
Pages :
550-558
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE]
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