Article (Scientific journals)
Visualization of chromatin in the yeast nucleus and nucleolus using hyperosmotic shock
Thelen, Nicolas; Defourny, Jean; Lafontaine, Denis et al.
2021In International Journal of Molecular Sciences
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Keywords :
yeast; nucleus; chromatin; nucleolus; perichromatin granules; electron cytochemistry; immunoelectron microscopy
Abstract :
[en] Unlike in most eukaryotic cells, the genetic information of budding yeast in the exponential growth phase is only present in the form of decondensed chromatin, a configuration that does not allow its visualization in cell nuclei conventionally prepared for transmission electron microscopy. In this work, we have studied the distribution of chromatin and its relationships to the nucleolus using different cytochemical and immunocytological approaches applied to yeast cells subjected to hyperosmotic shock. Our results show that osmotic shock induces the formation of heterochromatin patches in the nucleoplasm and intranucleolar regions of the yeast nucleus. In the nucleolus, we further reveal the presence of osmotic shock-resistant DNA in the fibrillar cords which, in places, take on a pinnate appearance reminiscent of ribosomal genes in active transcription as observed after molecular spreading (‘Christmas trees’). We have also identified chromatin-associated granules whose size, composition, and behaviour after osmotic shock are reminiscent to that of mammalian perichromatin granules. Altogether, these data reveal it is possible to visualize heterochromatin in yeast and suggest the yeast nucleus displays a less-well compartmentalized organization than that of mammals.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Thelen, Nicolas  ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire
Defourny, Jean  ;  Université de Liège - ULiège > Neurosciences-Cellular and tissular biology
Lafontaine, Denis;  Université Libre de Bruxelles - ULB
Thiry, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire
Language :
English
Title :
Visualization of chromatin in the yeast nucleus and nucleolus using hyperosmotic shock
Publication date :
24 January 2021
Journal title :
International Journal of Molecular Sciences
ISSN :
1661-6596
eISSN :
1422-0067
Publisher :
Multidisciplinary Digital Publishing Institute (MDPI), Switzerland
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 19 January 2021

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