[en] Mass spectrometry imaging of complex biological samples often require high spectral resolution and mass accuracy to properly distinguish all isobaric compounds. To achieve such requirement dynamically harmonized FT-ICR analysers offer best results. However, mass accuracy below expectation due to masses shifting between pixels observed when performing image acquisitions. In this work we show a link between the mass shifting phenomenon andd the large variations observed in total ion current during image acquisitions. This work propose solve the mass shift by optimisation in sample preparation and acquisition parameters to stabilise the fluctuation of the total ion current in FT-ICR-MSI.
Research Center/Unit :
Laboratoire de spectrométrie de masse
Disciplines :
Chemistry
Author, co-author :
Tiquet, Mathieu ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique
La Rocca, Raphaël ; Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)
Far, Johann ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique
Quinton, Loïc ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie biologique
De Pauw, Edwin ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique
Other collaborator :
van Kruining, Daan; Universiteit Maastricht - UM > Department of Cognitive Neuroscience
Martinez-Martinez, Pilar; Universiteit Maastricht - UM > MHeNS School for Mental Health and Neuroscience
Language :
English
Title :
Effect of ion counts stability during dynamically harmonized FT-ICR-MSI
Alternative titles :
[fr] Effet de la stabilité du courrant total d'ion en imagerie FT-ICR à harmonisation dynamique
Publication date :
01 April 2019
Number of pages :
A0
Event name :
4th NVMS-BSMS conference
Event organizer :
Belgian Society for Mass Spectrometry Nederlandse Vereniging voor Massaspectrometrie
