Characterization of an immunodominant epitope in the endodomain of the coronavirus membrane protein

Coronavirus; Endodomain; Immunodominant epitope; Membrane protein; M protein, Coronavirus; Article; Western blotting; Bagg albino mouse; Transmissible gastroenteritis virus; Animals; Antibodies, Monoclonal; Antibodies, Viral; Blotting, Western; Cell Line; Epitope Mapping; Fluorescent Antibody Technique; Immunodominant Epitopes; Mice, Inbred BALB C; Swine; Viral Matrix Proteins

Abstract :

[en] The coronavirus membrane (M) protein acts as a dominant immunogen and is a major player in virus assembly. In this study, we prepared two monoclonal antibodies (mAbs; 1C3 and 4C7) directed against the transmissible gastroenteritis virus (TGEV) M protein. The 1C3 and 4C7 mAbs both reacted with the native TGEV M protein in western blotting and immunofluorescence (IFA) assays. Two linear epitopes, 243YSTEART249 (1C3) and 243YSTEARTDNLSEQEKLLHMV262 (4C7), were identified in the endodomain of the TGEV M protein. The 1C3 mAb can be used for the detection of the TGEV M protein in different assays. An IFA method for the detection of TGEV M protein was optimized using mAb 1C3. Furthermore, the ability of the epitope identified in this study to stimulate antibody production was also evaluated. An immunodominant epitope in the TGEV membrane protein endodomain was identified. The results of this study have implications for further research on TGEV replication. © 2016 by the authors; licensee MDPI, Basel, Switzerland.

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Dong, Hui ; Université de Liège - ULiège > Doct. sc. agro. & ingé. biol. (Paysage)

Zhang, X.; State Key Laboratory of Veterinary Biotechnology, Division of Swine Infectious Diseases, Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences, Harbin, China

Shi, H.; State Key Laboratory of Veterinary Biotechnology, Division of Swine Infectious Diseases, Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences, Harbin, China

Chen, J.; State Key Laboratory of Veterinary Biotechnology, Division of Swine Infectious Diseases, Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences, Harbin, China

Da, S.; State Key Laboratory of Veterinary Biotechnology, Division of Swine Infectious Diseases, Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences, Harbin, China

Zhu, Y.; State Key Laboratory of Veterinary Biotechnology, Division of Swine Infectious Diseases, Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences, Harbin, China

Li, F.; State Key Laboratory of Veterinary Biotechnology, Division of Swine Infectious Diseases, Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences, Harbin, China

Language :

English

Title :

Characterization of an immunodominant epitope in the endodomain of the coronavirus membrane protein

Publication date :

2016

Journal title :

Viruses

eISSN :

1999-4915

Publisher :

Multidisciplinary Digital Publishing Institute (MDPI), Switzerland

Volume :

Issue :

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

NSCF - National Natural Science Foundation of China [CN]

Funding text :

2015BAD12B02; 31572541, NSFC, National Natural Science Foundation of China; 31502092, NSFC, National Natural Science Foundation of China

Available on ORBi :

since 26 December 2020

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